The Effect of Lactic Acid Bacteria and Bifidobacteria on the Number of Natural Killer Cells in Normal Conditions and in Cases of Intravaginal Staphylococcosis in Mice




Lactic acid bacteria, Bifidobacteria, Natural killer cells, Spleen, Intravaginal staphylococcosis, Mice


Background. Development of new immunobiotics based on commensal nonpathogenic probiotic bacteria such as lactic acid bacteria and bifidobacteria with antibacterial and immunomodulatory effects is an important area of modern biotechnology.

Оbjective. The aim of this study was to determine the effect of Lactobacillus acidophilus IMV B-7279, L. casei IMV B-7280L. delbrueckii subsp. bulgaricus ІМV В-7281, Bifidobacterium animalis VKL and B. animalis VKB (individually) or their different compositions on the number of natural killer cells (NKC) in the spleen of BALB/c mice at normal conditions and in the case of the experimental intravaginal staphylococcosis.

Methods. The number of NKC in the spleen was studied using monoclonal phycoerythrin-conjugated antibodies against NKC antigens (MACS, Miltenyi Biotec, Germany). Calculations of NKC as well as analysis of the results were performed using flow cytometry method on a FACStar Plus cytofluorometer.

Results. It is shown that the number of NKC in the spleen of intact mice did not change under the influence of L. acidophilus ІМV В-7279, L. casei ІМV В-7280, B. animalis VKL or B. animalis VKB (individually). But, using L. acido­philus ІМV В-7279, L. casei ІМV В-7280, L. delbrueckii subsp. bulgaricus ІМV В-7281, B. animalis VKL and B. ani­malis VKB (individually) or their different compositions for colonization of the vagina in the case of intravaginal staphylococcosis associated with increasing of the number of NKC in spleen in different periods of observation. The number of NKC in the spleen of staphylococcus-infected mice completely normalized after treatment with some probiotic compositions. The probiotic bacteria (individually) only partially normalized the number of NKC in the spleen of staphylococcus-infected mice.

Conclusions. Thus, L. acidophilus ІМV В-7279, L. casei ІМV В-7280, L. delbrueckii subsp. bulgaricus ІМV В-7281 or B. animalis VKL (individually) or their various compositions are promising to create highly effective immunobiotics, that are able to increase the innate immunity in cases of infections.


Lodoen MB, Lanier LL. Natural killer cells as an initial defense against pathogens. Curr Opin Immunol. 2006 Aug;18(4):391-8. DOI 10.1016/j.coi.2006.05.002

Schmidt S, Ullrich E, Bochennek K, Zimmermann SY, Lehrnbecher T. Role of natural killer cells in antibacterial immunity. Expert Rev Hematol. 2016 Dec;9(12):1119-27. DOI 10.1080/17474086.2016.1254546

Cooper MA, Fehniger TA, Fuchs A, Colonna M, Caligiuri MA. NK cell and DC interactions. Trends Immunol. 2004 Jan;25(1):47-52. DOI 10.1016/

Reinhardt R, Pohlmann S, Kleinertz H, Hepner-Schefczyk M, Paul A, Flohé SB. Invasive surgery impairs the regulatory function of human CD56 bright natural killer cells in response to Staphylococcus aureus. Suppression of Interferon-γ syn-thesis. PLoS One. 2015 Jun 19;10(6):e0130155. DOI 10.1371/journal.pone.0130155

Sha WH, Zeng XH, Min L. The correlation between NK cell and liver function in patients with primary hepatocellular carcinoma. Gut Liver. 2014 May;8(3):298-305. DOI 10.5009/gnl.2014.8.3.298

Bruunsgaard H, Pedersen AN, Schroll M, Skinhøj P, Pedersen BK. Decreased natural killer cell activity is associated with atherosclerosis in elderly humans. Exp Gerontol. 2001 Dec;37(1):127-36. DOI 10.1016/S0531-5565(01)00162-0

Ojo-Amaize EA, Conley EJ, Peter JB. Decreased natural killer cell activity is associated with severity of chronic fatigue immune dysfunction syndrome. Clin Infect Dis. 1994 Jan;18 Suppl 1:S157-9.

Furue H, Matsuo K, Kumimoto H, Hiraki A, Suzuki T, Yatabe Y, et al. Decreased risk of colorectal cancer with the high natural killer cell activity NKG2D genotype in Japanese. Carcinogenesis. 2008 Feb;29(2):316-20. DOI 10.1093/carcin/bgm260

Small CL, McCormick S, Gill N, Kugathasan K, Santosuosso M, Donaldson N, et al. NKC play a critical protective role in host defense against acute extracellular Staphylococcus aureus bacterial infection in the lung. J Immunol. 2008 Apr 15;180(8):5558-68. DOI 10.4049/jimmunol.180.8.5558

Shirtliff ME, Mader JT. Acute septic arthritis. Clin Microbiol Rev. 2002 Oct;15(4):527-44. DOI 10.1128/CMR.15.4.527-544.2002

Hiramatsu K, Cui L, Kuroda M, Ito T. The emergence and evolution of methicillin-resistant Staphylococcus aureus. Trends Microbiol. 2001 Oct;9(10):486-93.

Zhao H, Li W, Gao Y, Li J, Wang H. Exposure to particular matter increases susceptibility to respiratory Staphylococcus aureus infection in rats via reducing pulmonary natural killer cells. Toxicology. 2014 Nov 5;325:180-8. DOI 10.1016/j.tox.2014.09.006

Nilsson N, Bremell T, Tarkowski A, Carlsten H. Protective role of NK1.1+ cells in experimental Staphylococcus aureus arthritis. Clin Exp Immunol. 1999 Jul;117(1):63-9. DOI 10.1046/j.1365-2249.1999.00922.x

Amdekar S, Singh V, Singh DD. Probiotic therapy: immunomodulating approach toward urinary tract infection. Curr Microbiol. 2011 Nov;63(5):484-90. DOI 10.1007/s00284-011-0006-2

Frei R, Akdis M, O'Mahony L. Prebiotics, probiotics, synbiotics, and the immune system: experimental data and clinical evidence. Curr Opin Gastroenterol. 2015 Mar;31(2):153-8. DOI 10.1097/MOG.0000000000000151

Fink LN, Zeuthen LH, Christensen HR, Morandi B, Frøkiaer H, Ferlazzo G. Distinct gut-derived lactic acid bacteria elicit divergent dendritic cell-mediated NK cell responses. Int Immunol. 2007 Dec;19(12):1319-27. DOI 10.1093/intimm/dxm103

Rizzello V, Bonaccorsi I, Dongarrà ML, Fink LN, Ferlazzo G. Role of natural killer and dendritic cell crosstalk in immunomodulation by commensal bacteria probiotics. J Biomed Biotechnol. 2011;2011:473097. DOI 10.1155/2011/473097

Kawahara T, Takahashi T, Oishi K, Tanaka H, Masuda M, Takahashi S, et al. Consecutive oral administration of Bifidobacterium longum MM-2 improves the defense system against influenza virus infection by enhancing natural killer cell activity in a murine model. Microbiol Immunol. 2015 Jan;59(1):1-12. DOI 10.1111/1348-0421.12210

Fan J, Hou Y, Zhou S, Cai X. Effect of Bifidobacterium on the immunity in BALB/c mice. Wei Sheng Wu Xue Bao. 2015 Apr 4;55(4):484-91.

Lazarenko L, Babenko L, Sichel LS, Pidgorskyi V, Mokrozub V, Voronkova O, et al. Antagonistic action of Lactobacilli and Bifidobacteria in relation to Staphylococcus aureus and their influence on the immune response in cases of intravaginal staphylococcosis in mice. Probiotics Antimicrob Proteins. 2012 Jun;4(2):78-89. DOI 10.1007/s12602-012-9093-z

Spivak MYa, Pidgorsky VS, Lazarenko LM, Shynkarenko LM, Rachkova LT, Olevinska ZM. Lactobacillus and Bifidobacterium influence the indices of immune response of the organism showed on experimental model. Microbiol Biotechnol. 2009;1(5):39-46.

Мokrozub VV, Lazarenko LM, Sichel LM, Babenko LP, Lytvyn PM, Demchenko OM, et al. The role of beneficial bacteria wall elasticity in regulating innate immune response. EPMA J. 2015 Jun 19;6(1):13. DOI 10.1186/s13167-015-0035-1

Koizumi S, Wakita D, Sato T, Mitamura R, Izumo T, Shibata H, et al. Essential role of Toll-like receptors for dendritic cell and NK1.1(+) cell-dependent activation of type 1 immunity by Lactobacillus pentosus strain S-PT84. Immunol Lett. 2008 Oct 30;120(1-2):14-9. DOI 10.1016/j.imlet. 2008.06.003

Fukui Y, Sasaki E, Fuke N, Nakai Y, Ishijima T, Abe K, et al. Effect of Lactobacillus brevis KB290 on the cell-mediated cytotoxic activity of mouse splenocytes: a DNA microarray analysis. Br J Nutr. 2013 Nov 14;110(9):1617-29. DOI 10.1017/S0007114513000767

Kou X, Chen Q, Ju X, Liu H, Chen W, Xue Z. A tolerant lactic acid bacteria, Lactobacillus paracasei, and its immuno-regulatory function. Can J Microbiol. 2014 Nov;60(11):729-36. DOI 10.1139/cjm-2014-0383

Lee YD, Hong YF, Jeon B, Jung BJ, Chung DK, Kim H. Differential cytokine regulatory effect of three Lactobacillus strains isolated from fermented foods. J Microbiol Biotechnol. 2016 Sep 28;26(9):1517-26. DOI 10.4014/jmb.1601.01044

Takagi A, Matsuzaki T, Sato M, Nomoto K, Morotomi M, Yokokura T. Enhancement of natural killer cytotoxicity delayed murine carcinogenesis by a probiotic microorganism. Carcinogenesis. 2001 Apr;22(4):599-605. DOI 10.1093/carcin/22.4.599

Lee JW, Shin JG, Kim EH, Kang HE, Yim IB, Kim JY, et al. Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum. J Vet Sci. 2004 Mar;5(1):41-8.

Soltan Dallal MM, Yazdi MH, Holakuyee M, Hassan ZM, Abolhassani M, Mahdavi M. Lactobacillus casei ssp. casei induced Th1 cytokine profile and natural killer cells activity in invasive ductal carcinoma bearing mice. Iran J Allergy Asthma Immunol. 2012 Jun;11(2):183-9. DOI 011.02/ijaai.183189




How to Cite

Lazarenko LM, Babenko L, Mokrozub V, Voronkevich M, Loseva D, Sichel L, Spivak M. The Effect of Lactic Acid Bacteria and Bifidobacteria on the Number of Natural Killer Cells in Normal Conditions and in Cases of Intravaginal Staphylococcosis in Mice. Innov Biosyst Bioeng [Internet]. 2017Dec.19 [cited 2024May28];1(1):18-26. Available from: