Innovative Biosystems and Bioengineering <p>The scientific journal <em>Innovative Biosystems and Bioengineering</em> was founded in 2017. IBB introduces a systems approach to life sciences problems.</p> <p>IBB is a quarterly peer-reviewed Open Access e-journal in which readers, immediately upon online publication, can access articles free of costs and subscription charges.</p> <p>e-ISSN 2616-177X</p> <p>Founder and Publisher: National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”.</p> <p>Frequency: 4 issues a year.</p> <p>We accept papers in following languages: English, Ukrainian.</p> <p>Cite the title as: Innov Biosyst Bioeng.</p> <p>Readership: Biotechnologists, Bioengineers, Biomedical researchers and engineers, Biologists.</p> <p>Indexing: Scopus; DOAJ; ROAD; HINARI; Chemical Abstracts Service; CNKI Scholar; Norwegian Register for Scientific Journals, Series and Publishers; J-Gate; Public Knowledge Project Index; ICMJE; JournalTOCs; WCOSJ; Vifabio; EZB; Federation of Finnish Learned Societies; Zeitschriftendatenbank; Polska Bibliografia Naukowa; Scilit; Bielefeld Academic Search Engine; OpenAir; WorldCat.</p> Igor Sikorsky Kyiv Polytechnic Institute en-US Innovative Biosystems and Bioengineering 2616-177X <p><span>The ownership of copyright remains with the Authors.</span></p><p>Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.</p><p>Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.</p>IBB articles are published under Creative Commons licence:<br /><ol type="a"><li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under <a href="">CC BY 4.0</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.<br /><br /></li><li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.<br /><br /></li><li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.</li></ol> Systems for Genetic Assessment of the Impact of Environmental Factors <p>One of the most important components of environmental protection is the development of hygiene standards aimed at shielding the human population from the adverse effects of environmental pollution. The European and American Chemical Societies have reported approximately 800,000 chemicals, with no available information on potential risks to human genetic health and negative environmental impact. Given the exponential increase in chemical compounds generated by humanity in various industries, the issue of effectivly identifying and accounting for various genetic and carcinogenic hazards is particularle relevant. The assessment of potential genotoxicity of environmental factors is an integral part of genetic safety assessment for both prokaryotic and eukaryotic organisms, including humans. The evaluation of the genetic activity of chemical compounds is a fundamentsl requirement for their comprehensive toxicological assessment. From the perspective of genetic and epigenetic mechanisms of influence, our review considers standard methods for detecting and assessing the potential genetic hazard associated with environmental factors. These methods are part of a standard<strong>, </strong>generally accepted test system battery. Additionally, the review covers some modern experimental methods that are not widely accepted today. A detailed analysis of approaches to the assessment of potential genetic mutagenic activity was carried out, presenting their main advantages and disadvantages. Taking into account the recommendations issued by the Organisation for Economic Co-operation and Deve­lopment on testing hazardous chemical compounds that may affect human health, an attempt was made to find optimal approaches to solving the task of predicting genetic effects and their consequences for humans.</p> Sergey Kislyak Olexii Dugan Olena Yalovenko Copyright (c) 2024 The Author(s) 2024-03-25 2024-03-25 8 2 3 27 10.20535/ibb.2024.8.2.288127 The Role of reactive Oxygen Species in the Implementation of the Anti-Tumor Effect of Nanocomplexes Based on GdEuVO4 Nanoparticles and Cholesterol <p><strong>Background.</strong> An experimental study of the antitumor effect of nanocomplexes (NCs) consisting of GdYEuVO<sub>4</sub> nanoparticles and cholesterol indicates their potential use in oncological practice. The mechanism of the antitumor effect of NCs may be associated with the formation of reactive oxygen species (ROS), leading to subsequent tumor cell death.</p> <p><strong>Objective.</strong> To study the pro-oxidant and antitumor properties of NCs consisting of GdYEuVO<sub>4</sub> nanoparticles and cholesterol in an <em>in vitro</em> system.</p> <p><strong>Methods.</strong> Experiments were performed on Ehrlich ascites carcinoma (EAC) cells, which were introduced intraperitoneally into BALB/c mice. On the 7th day of EAC development, cells were isolated and treated with NCs for 3 hours <em>in vitro</em>. Untreated cells served as the control. The formation of intracellular ROS was quantified by flow cytometry using the Fluorometric Intracellular ROS Kit. The metabolic activity of EAC cells was assessed using the colorimetric MTT test. The number of cells undergoing apoptosis or necrosis was evaluated using flow cytometry and the FITC Annexin V Apoptosis Detection Kit I.</p> <p><strong>Results.</strong> Incubation of EAC cells with NCs resulted in more than a 3-fold increase in ROS formation compared to the control. NCs also caused almost a two-fold inhibition of the metabolic activity of EAC cells, accompanied by a 25% decrease in the number of viable EAC cells. It was shown that NCs are unique compounds capable of simultaneously inducing several types of cell death, with necrosis being the predominant mode of tumor cell death after NC treatment.</p> <p><strong>Conclusions. </strong>The cytotoxic effect of NCs on tumor cells is mediated through their pro-oxidant properties. These results can be considered when developing new cancer therapy strategies.</p> Anatoliy Goltsev Mykola Bondarovych Yuliia Gaevska Nataly Babenko Tetyna Dubrava Maksym Ostankov Copyright (c) 2024 The Author(s) 2024-05-31 2024-05-31 8 2 28 37 10.20535/ibb.2024.8.2.295581 In vitro Modeling of the Effect of Lactobacillus Metabolites on the Systemic Response of the Body in Intestinal Viral Infection <p><strong>Background.</strong> Viral infectious diseases remain a significant cause of morbidity and mortality worldwide. The lack of effective antiviral drugs for the treatment of viral gastroenteritis emphasizes the relevance of various forms of combination therapy, including a balanced diet and the use of probiotics.</p> <p><strong>Objective.</strong> To verify <em>in vitro</em> the hypothesis about the effect of metabolites from probiotic strains of lactobacilli on the systemic response of the body in intestinal viral infection.</p> <p><strong>Methods.</strong> The objects of study were filtrates of culture fluids from probiotic lactobacillus strains <em>L. delbrueckii</em> <em>subsp. lactis</em> LE and <em>L. rhamnosus</em> LB3. HEp-2 and Namalwa cell cultures and vesicular stomatitis test virus were used as biological test objects. The study employed spectrophotometric and cytofluorometric analysis.</p> <p><strong>Results.</strong> The absence of a direct anti-inflammatory effect in the samples of lactobacillus culture fluid filtrates was revealed. It was found that the culture fluid filtrate of the lactobacillus strain <em>L. delbrueckii subsp. lactis</em> LE exhibited the most pronounced properties of an interferon coinducer. The proliferative index (PI) of HEp-2 cells was analyzed under different effects. Cells incubated with lactobacillus culture fluid filtrate (LE) showed an increase in PI for young cells, a decrease for mid-mature cells, and an increase for late-mature cells. The combined effect of TNF-a and lactobacillus LE culture filtrate was characterized by stimulation of PI for medium-mature cells and significant inhibition of PI of late-mature cells.</p> <p><strong>Conclusions.</strong> The synergistic effect of lactobacillus culture filtrates and inflammatory factors will contribute to the accelerated elimination of late-mature and affected cells from the intestine and stimulate the proliferation of young and medium-mature cells to replace them, thereby promoting the regeneration of the intestinal epithelium and accelerate recovery. At the same time, probiotic strains of lactobacilli can enhance the interferonogenic properties of gastroenteritis viruses, and ultimately stimulating the formation of specific immunity in these diseases.</p> Serhii Soloviov Olena Trokhimenko Valentyna Polishchuk Vadym Pits Volodymyr Vasylenko Yevheniia Vasylenko Illia Hol Artem Symchuk Olena Kostiuk Copyright (c) 2024 The Author(s) 2024-06-21 2024-06-21 8 2 38 52 10.20535/ibb.2024.8.2.306587 Recognition and Categorization of Blood Groups by Machine Learning and Image Processing Method <p><strong>Background. </strong>Red blood cells are one of the components of blood. Blood is an important fluid in the human body. Knowing the blood groups is essential in blood transfusion operations, which depend on fixed conditions to avoid fatal errors. The method that is used to determine the blood groups is a traditional method that relies on medical laboratory technicians, as it is subject to human errors.</p> <p><strong>Objective.</strong> This paper aims to design and implement a prototype to detect and classify blood groups to avoid human error in blood group detection. The proposed system employs image processing and machine learning algorithms for blood group detection and classification.</p> <p><strong>Methods.</strong> The system consists of three stages. First, samples were collected from volunteers. Second, images of the samples were captured using a camera. Third, the images were analyzed using two methods: image proces­sing via MATLAB and machine learning algorithms via Orange, for blood group detection and classification.</p> <p><strong>Results. </strong>The accuracy in processing images using the MATLAB program reached 100%, with processing time ranged from 1.5 to 1.6 seconds. Additionally, using machine learning with neural networks in the Orange program, the accuracy was 99.7%, with training times of 13.7 seconds and testing times of 1.2 seconds. Neural networks outperformed other models, as shown in the experimental results. The study concluded that automated blood type detection using image processing and machine learning methods is effective and feasible compared to manual methods. The proposed system outperformed previous studies in terms of accuracy, processing time, training time, and testing time using both methods.</p> <p><strong>Conclusions. </strong>This study underscores the urgent need for precise blood type determination before emergency blood transfusions, which currently relies on manual inspection and is susceptible to human errors. These errors have the potential to endanger lives during blood transfusions. The main goal of the research was to develop an approach that combines image processing and machine learning to accurately classify blood groups.</p> Mustafa F. Mahmood Copyright (c) 2024 The Author(s) 2024-06-17 2024-06-17 8 2 53 68 10.20535/ibb.2024.8.2.298201 Evaluating the Interaction Between Silicon Surface and Microorganisms in Various Solvents Under the Influence of a Static Magnetic Field Using Fractal Analysis <p><strong>Background.</strong> Peculiarities of the interaction of microorganisms with the surface are important from the point of view of the functionality of this surface (implants, chips, electrodes with biofilm for producing electric current). The orderliness of organic particles and cells on different surfaces can be assessed by determining the fractal dimension and lacunarity and indicate the structural state or efficiency of the system.</p> <p><strong>Objective.</strong> Investigation how different solvents and the application of a magnetic field affect the texture of suspensions containing microorganisms when dried on various types of silicon surfaces and quantitatively assess the dimensions of the structures formed using fractal analysis.</p> <p><strong>Methodology.</strong> After mixing, the cell suspension was applied to the polished, degreased surface of silicon wafers arranged horizontally and left to dry completely. In a static magnetic field (MF) with an induction of 0.17 T, the induction lines were perpendicular to the surface of the sample. Micrographs of dried cells were pro­cessed in software package ImageJ and fractal analysis was performed using the FracLac software application and "Box counting" technique.</p> <p><strong>Results.</strong> Significant differences in the self-organization of various types of microorganism cells during drying on silicon surfaces under the influence of a MF and in different solvents have been found. The tendency for various types of microorganisms was the formation of pseudofractal shapes and an increase in the average fractal dimension <em>D</em> under the action of a MF. As <em>D</em> increased, lacunarity <em>L</em> decreased. However, in the case of yeast suspended in a physiological solution, pseudofractal shapes were observed even in the absence of a MF.</p> <p><strong>Conclusions.</strong> Using fractal analysis of pseudofractal figures consisting of cells of microorganisms on the surface of a silicon plate under the influence of MF, it is possible to evaluate the functionality of cells interacting with the surface, as well as the quality of this surface.</p> Lolita Marynchenko Olena Nizhelska Aram Shirinyan Nadezhda Gorchakova Copyright (c) 2024 The Author(s) 2024-06-21 2024-06-21 8 2 69 84 10.20535/ibb.2024.8.2.297364