Innovative Biosystems and Bioengineering 2021-09-29T15:10:17+03:00 Liudmyla Trotsenko Open Journal Systems <p>The international scientific journal Innovative Biosystems and Bioengineering was founded in 2017. IBB introduces a systems approach to life sciences problems.</p> <p>IBB is a quarterly peer-reviewed Open Access e-journal in which readers, immediately upon online publication, can access articles free of costs and subscription charges.</p> <p>e-ISSN 2616-177X</p> <p>Founder: National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”.</p> <p>Frequency: 4 issues a year.</p> <p>We accept papers in following languages: English, Ukrainian.</p> <p>Cite the title as: Innov Biosyst Bioeng.</p> <p>Readership: Biotechnologists, Biologists, Biophysicists, Bioinformaticists, Biomedical researchers and engineers, Medical, biochemical, and pharmaceutical scientists.</p> <p>Keywords: Applied biology, Biotechnology, Bioengineering, Biophysics, Bioinformatics, Bioorganic chemistry, Biomedicine, Biochemical engineering, Biomaterials, Bioprocess engineering.</p> <p>Indexing: DOAJ; ROAD; HINARI; Chemical Abstracts Service; CNKI Scholar; Norwegian Register for Scientific Journals, Series and Publishers; J-Gate; Public Knowledge Project Index; ICMJE; JournalTOCs; WCOSJ; Vifabio; EZB; Federation of Finnish Learned Societies; Zeitschriftendatenbank; Polska Bibliografia Naukowa; Scilit; Bielefeld Academic Search Engine; OpenAir; WorldCat.</p> An Experimental Study of the Cryopreserved Placenta Extract Effect On the Sodium Diclofenac Anti-Inflammatory Activity 2021-07-19T08:28:50+03:00 Fedir Hladkykh <p><strong>Background.</strong> As a means of correcting the ulcerogenic effect of nonsteroidal anti-inflammatory drugs, our attention was attracted by a cryopreserved extract of the human placenta, which has a multivector spectrum of biological activity. To date, there is no information about its effect on the specific activity of this class of medicines (anti-inflammatory, analgesic, etc.) in published sources.</p> <p><strong>Objective. </strong>We are aimed to characterize the effect of cryopreserved placenta extract on the anti-inflammatory activity of diclofenac sodium when administered separately in a model of acute exudative inflammation.</p> <p><strong>Methods.</strong> Experimental studies <em>in vivo</em> were conducted on 28 nonlinear male laboratory rats. The model of acute exudative inflammation was reproduced by subplantar injection of 0.1 ml of 1.0% aqueous solution of λ-karagenin into the right hind limb of rats. Cryopreserved placenta extract was administered intramuscularly at a dose of 0.16 ml/kg 60 minutes before diclofenac sodium (8 mg/kg).</p> <p><strong>Results.</strong> Preventive administration of diclofenac sodium caused an antiexudative effect as early as 30 minutes after administration of λ-karagenin – its anti-inflammatory activity was 11.0%, which is 4.6 times higher than similar indicators at the same time in rats injected with placental cryoextract. At 60 minutes of observation, diclofenac sodium was comparable in anti-inflammatory activity with cryopreserved placenta extract: 28.6% and 22.2%, respectively, but at 120 and 180 minutes, diclofenac sodium exceeded the studied cryoextract in antiphlogistic effect by 1.6 times in both periods of observation. The anti-inflammatory effect of the combined separate administration of placenta cryoextract and diclofenac sodium before λ-karagenin for 30 and 60 minutes was 12.7% and 32.3%, respectively, which is comparable with analogous indicators against the background of diclofenac sodium monotherapy. However, at 120 minutes of observation, the group of combined use of placenta cryoextract and diclofenac sodium showed the greatest anti-inflammatory effect among rats of all the studied groups – 52.6%, which was 2.2 times higher than the indicators of the placenta cryoextract monotherapy group and 1.4 times lower than the indicators of the rats of the diclofenac sodium monotherapy group.</p> <p><strong>Conclusions.</strong> 4 hours after administration, placental cryoextract had a suppressive effect on kinins like diclofenac sodium, and in the prostaglandin period of caragenin-induced inflammation against the background of combined use of the studied cryoextract and diclofenac sodium, the anti-inflammatory activity was 46.4 %. This suggests a suppressive effect on the production of prostaglandins as a possible mechanism of anti-exudative action of cryopreserved placenta extract.</p> 2021-09-15T00:00:00+03:00 Copyright (c) 2021 The Author(s) Comparative Analysis of Classification Algorithms in the Analysis of Medical Images From Speckle Tracking Echocardiography Video Data 2021-06-21T15:36:02+03:00 Olena Petrunina Diana Shevaga Vitalii Babenko Volodymyr Pavlov Sergiy Rysin Ievgen Nastenko <p><strong>Background.</strong> Machine learning allows applying various intelligent algorithms to produce diagnostic and/or prognostic models. Such models can be used to determine the functional state of the heart, which is diagnosed by speckle-tracking echocardiography. To determine the patient's heart condition in detail, a classification approach is used in machine learning. Each of the classification algorithms has a different performance when applied to certain situations. Therefore, the actual task is to determine the most efficient algorithm for solving a specific task of classifying the patient's heart condition when applying the same speckle-tracking echocardiography data set.</p> <p><strong>Objective</strong><strong>.</strong> We are aimed to evaluate the effectiveness of the application of prognostic models of logistic regression, the group method of data handling (GMDH), random forest, and adaptive boosting (AdaBoost) in the construction of algorithms to support medical decision-making on the diagnosis of coronary heart disease.</p> <p><strong>Methods</strong><strong>.</strong> Video data from speckle-tracking echocardiography of 40 patients with coronary heart disease and 16 patients without cardiac pathology were used for the study. Echocardiography was recorded in B-mode in three positions: long axis, 4-chamber, and 2-chamber. Echocardiography frames that reflect the systole and diastole of the heart (308 samples in total) were taken as objects for classification. To obtain informative features of the selected objects, the genetic GMDH approach was applied to identify the best structure of harmonic textural features. We compared the efficiency of the following classification algorithms: logistic regression method, GMDH classifier, random forest method, and AdaBoost method.</p> <p><strong>Results</strong><strong>.</strong> Four classification models were constructed for each of the three B-mode echocardiography positions. For this purpose, the data samples were divided into 3: training sample (60%), validation sample (20%), and test sample (20%). Objective evaluation of the models on the test sample showed that the best classification method was random forest (90.3% accuracy on the 4-chamber echocardiography position, 74.2% on the 2-chamber, and 77.4% on the long axis). This was also confirmed by ROC analysis, wherein in all cases, the random forest was the most effective in classifying cardiac conditions.</p> <p><strong>Conclusions.</strong> The best classification algorithm for cardiac diagnostics by speckle-tracking echocardiography was determined. It turned out to be a random forest, which can be explained by the ensemble approach of begging, which is inherent in this classification method. It will be the mainstay of further research, which is planned to be performed to develop a full-fledged decision support system for cardiac diagnostics.</p> 2021-09-10T00:00:00+03:00 Copyright (c) 2021 The Author(s) The Role of Cord Blood in the Regulation of the Cellular and Humoral Link of Immunity in Experimental Atopic Dermatitis 2021-08-16T17:08:29+03:00 Hanna Koval Olena Lutsenko Mykola Bondarovych Maksym Ostankov Anatoliy Goltsev <p><strong>Background.</strong> Atopic dermatitis (AD) as one of the most common diseases of autoimmune genesis in the structure of dermatological practice, is characterized by itching, dryness, thickening of the skin, characteristic rashes. The drugs of choice in the treatment of AD are steroidal anti-inflammatory drugs. However, the development of unwanted side effects is a serious problem attributed to using hormone therapy. The search for effective methods of treating AD is an urgent task of medicine and in particular dermatology. At the same time, there is an obvious need for the participation in the solution of this problem also of specialists-immunologists working in the field of application of cell therapy drugs, acting on various pathogenetic links of the disease. The development of new or optimization of existing methods of treating AD is the urgent task facing them.</p> <p><strong>Objective.</strong> Evaluation of the immunocorrective effect of lyophilized (lHCBL) and cryopreserved human cord blood leucoconcetrate (cHCBL) on a AD model.</p> <p><strong>Methods.</strong> The experiments were carried out on 6-month-old Wistar rats. Upon induction of AD, the inflammation focus was formed on the rat's back (9–10 cm<sup>2</sup>) by daily rubbing in a 5% alcohol-acetone solution of dinitrochlorobenzene (DNCB) for 21 days. cHCBL and lHCBL were injected intraperitoneally, 0.5 ml at a dose of 5´10<sup>6</sup> cells in one day after the final DNCB treatment. The adhesive and phagocytic activity of the cells of the peritoneal cavity, the level of circulating immune complexes, the population and subpopulation of lymphocytes (CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup>, CD16<sup>+</sup>, CD4<sup>+</sup>CD25<sup>+</sup>), the immunoregulatory index of lymphocytes, the concentration of immunoglobulins in the blood serum were determined.</p> <p><strong>Results.</strong> For AD induced by DNCB, systemic changes in the immune status are characteristic, which is expressed by changes in the parameters of cellular and humoral immunity. The most fundamental changes in cell subpopulations in spleen of rats with AD were revealed: a decrease in the number of total T-lymphocytes and their two main subpopulations (CD4<sup>+ </sup>and CD8<sup>+ </sup>cells). Against this background, changes were noted in the monocytic-phagocytic and humoral systems of immunity. The paper shows the effectiveness of the use of cHCBL and lHCBL in the correction of pathological manifestations of experimental AD. On the background of treatment, the features of the immunocorrective effect of each of the drugs were noted. Thus, when assessing intergroup values, a more pronounced increase in T-reg was revealed in rats of the 5th group – 3.9 [3.8; 4.0] versus 3.2 [3.0; 3.3] in the 4th group (<em>P</em> &lt; 0.01); IgA level – 1.6 [1.5; 1.7] versus 1.3 [1.2; 1.4] (<em>P</em> &lt; 0.01).</p> <p><strong>Conclusions.</strong> Thus, lHCBL exhibits immunocorrective activity in the treatment of experimental AD, surpassing in some parameters the activity of сHCBL, which is promising for its use in clinical practice.</p> 2021-09-22T00:00:00+03:00 Copyright (c) 2021 The Author(s) The Effect of Magnetite Nanoparticles on the Growth and Development of Nicotiana Tabacum Plants in Vivo and in Vitro Culture 2021-06-02T11:50:01+03:00 Svitlana Gorobets Nina Ilchuk Iryna Demianenko Maria Bannikova <p><strong>Background.</strong> Nanomaterials are easily modified and have unique characteristics associated with a large reactive surface Due to these properties, nanomaterials are used in various branches of sciences and technology, such as pharmaceuticals, biotechnology, chemical technology, etc. Recently, the effect of magnetite nanoparticles on the morphological properties of plants has been actively studied for their further use as nanoadditives to increase yields and improve the properties of agricultural plants. Tobacco (Nicotiana tabacum) is a model object of plant biotechnology, it is used to study the effect of various factors on dicotyledonous plants, so it was chosen to study the effect of magnetite on the growth, development, and mass accumulation by plants.</p> <p><strong>Objective.</strong> We are aimed to study the effect of magnetite nanoparticles on the growth and development of <em>Nicotiana tabacum</em> <em>in vivo</em> and <em>in vitro</em>.</p> <p><strong>Methods.</strong> The ability of tobacco to produce biogenic magnetic nanoparticles by searching for mammal proteins homologues in the<br /><em>Nicotiana tabacum </em>proteome using the Blast NCBI program was studied using comparative genomics methods. The plants were divided into groups (control, magnetite nanoparticle concentration 0.1 mg/cm<sup>3</sup>, magnetite nanoparticle concentration 1 mg/cm<sup>3</sup>) for both <em>in vivo</em> and <em>in vitro</em> experiments. Analysis of plant parameters was performed every 14 days to study the dynamics of the effects of magnetite nanoparticles.</p> <p><strong>Results.</strong> It was determined that magnetite nanoparticles at a concentration of 0.1 mg/cm<sup>3</sup> in culture <em>in vitro</em> and <em>in vivo</em> significantly affect the growth of the root system and sprouts of <em>Nicotiana tabacum</em>. On the 56th day of plant cultivation <em>in vitro</em> on a salivary medium supplemented with magnetite nanoparticles at a concentration of 0.1 mg/cm<sup>3</sup>, an increase in the shoot length by 13.3%, root length by 31.7%, and the mass of absolutely dry substances by 18.75% was observed compared to the control. Treatment of magnetite nanoparticles with a suspension at a concentration of 0.1 mg/cm<sup>3</sup> led to more pronounced results when growing tobacco <em>in vivo</em>. So, on the<br />56th day, the root length increased by 23.3%, the length of the shoot – by 19.2%, and the mass of absolutely dry substances – by<br />2 times, the first leaves appeared 2 days earlier compared to the control. The addition of magnetite nanoparticles to the substrate on which the plants were grown <em>in vivo </em>at a concentration of 1 mg/cm<sup>3</sup> inhibits the growth of tobacco.</p> <p><strong>Conclusions.</strong> Studies have shown the expediency of using magnetic nanoparticles at a concentration of 0.1 mg/cm<sup>3</sup> as nanofertilizers in tobacco cultivation.</p> 2021-09-26T00:00:00+03:00 Copyright (c) 2021 The autror(s) Efficiency of Combined Use of Fullerene C60 and Bovine Serum Albumin for Rehabilitation of Vitrified Fragments of Rat Immature Seminiferous Tubules 2021-09-29T15:10:17+03:00 Nataliia Volkova Maria Yuhta Larisa Sokil Lyudmyla Chernyshenko Ludmila Stepanuk Anatoliy Goltsev <p><strong>Background.</strong> Today, cryopreserved reproductive tissues are used to treat some forms of male infertility. However, after long-term preservation of fragments of seminiferous tubules of testes (FSTT) in a low-temperature bank (-196 °С) their morphological and functional characteristics decrease reversibly. To solve this problem after freezing-thawing, the use of rehabilitation media with special additives is promising. Due to the fact that serum albumin and fullerene C60 have powerful protective and antioxidant properties, their use allows to stabilize the plasma membrane, osmotic pressure, and reduce free radicals that make them promising candidates to use in the development of rehabilitation media for biological objects after cryopreservation.</p> <p><strong>Objective. </strong>The efficacy of fullerene C60, bovine serum albumin (BSA), and their combination as components of rehabilitation medium of vitrified FSTT of immature rats was studied.</p> <p><strong>Methods.</strong> Vitrified-thawed samples of FSTT were incubated (22 °C) for 30 minutes in Leibovitz's medium with addition of 15 mg/mL C60, 5 g/L BSA or their combination. Control samples were incubated in the medium without C60 or BSA addition. Metabolic activity (MTT test), histomorphological data, total antioxidant status (TAS), reactive oxygen species (ROS) production, activity of g-glutamyltransferase (gGGT), and glucose-6-phosphate dehydrogenase were determined in the samples after rehabilitation in the investigated media.</p> <p><strong>Results. </strong>The use of C60 led to the increase of metabolic (by 1.26 times) and TAS (by 1.74 times) activities, to the decrease in the number of ROS+ cells (by 1.35 times) and to the improvement of the spermatogenic epithelium binding to the basement membrane versus control sample. Application of BSA did not significantly affect the studied biochemical indices but decreased the number of tubules with desquamation of spermatogenic epithelium in histological sections. The combined use of BSA and C60 had the best effect among investigated rehabilitation media that led to the increase of metabolic activity (by 1.51 times), TAS activity (by 1.78 times), gGGT activity (by 1.59 times), histostructure restoration and the decrease in the number of ROS+ cells (by 1.45 times) compared to the control samples.</p> <p><strong>Conclusions.</strong> The use of C60 and BSA combination increases the metabolic and antioxidant activity of vitrified FSTT and also has a positive effect on their histostructural characteristics compared to control samples. It should be noted that the effect of С60 and BSA addition to rehabilitation medium exceeds the results of using the investigated additives separately (by the metabolic and gGGT activity as well as architectonics of vitrified FSTT). These data relate to reproductive medicine and can be used to develop an effective rehabilitation protocol for vitrified FSTT.</p> 2021-09-29T00:00:00+03:00 Copyright (c) 2021 The autror(s) Prospects for the Development of Biomedical Engineering as an Educational and Scientific Field in Ukraine 2021-08-17T16:44:46+03:00 Alexander Galkin 2021-08-17T00:00:00+03:00 Copyright (c) 2021 The autror(s)