Screening of Strains of the Medicinal Mushroom Fomitopsis officinalis (vill.) Bondartsev & Singer Promising for Biotechnological Use

Authors

DOI:

https://doi.org/10.20535/ibb.2022.6.3-4.271383

Keywords:

Fomitopsis officinalis, liquid culture, mycelium mass, nutrient media

Abstract

Background. Macromycete cultivation methods development will contribute to the production of biotechnological products based on fungus. Determination of the main factors affecting medicinal macromycetes’ life processes allows to control biosynthetic activity of a fungal organism and obtain biotechnological products based on it.

Objective. Screening of Fomitopsis officinalis strains promising for biotechnological use, and determining of physico-chemical factors that affect the cultures life processes.

Methods. The objects of the study were three pure cultures of F. officinalis (IBK-2497, IBK-2498, IBK-5004). The influence of the acidity of the environment on the growth of mycelium, the needs of the cultures in the sources of carbon and nitrogen nutrition were determined. The following carbon sources were used: monosaccharides (glucose, xylose), disaccharides (sucrose, lactose) and trisaccharides (raffinose), polysaccharides (starch); nitrogen sources: KNO3, (NH4)2HPO4, asparagine, peptone. Dynamics of the culture growth were determined under the conditions of deep cultivation, on a liquid nutrient medium of glucose-peptone-yeast extract (GPA), g/l: glucose – 30.0; peptone – 3.5; yeast extract – 2.0; KH2PO4 – 1.0; K2HPO4 – 1.0; MgSO4×7H2O – 0.25.

Results. The pH range between 5.5 and 6.0 was the most favorable for active growth of all studied strains of F. officinalis. The best carbon sources for growth were glucose and starch; peptone and asparagine were the best source of nitrogen. Nutrient media with xylose, lactose and nitrate nitrogen were least suitable for growth. Analysis of the strains growth dynamics on the GPA medium showed that the largest mass of mycelium (up to 11.54 ± 0.2 g/l) was produced by culture F. officinalis IBK-5004 on the 10-th day of cultivation. Cultures F. officinalis IBK-2497, IBK-2498 grew slower, and the mycelial mass was 10.33 ± 0.2 and 9.68 ± 0.3 g/l on the 14-th day of cultivation.

Conclusions. Based on the obtained data, the F. officinalis IBK-5004 strain was selected. It can be considered a promising mycelial mass producer based on the set of characteristics.

References

Badalyan SM, Rapior S. Perspectives of biomedical application of macrofungi. Curr Trends Biomedical Eng Biosci. 2020;19(5):556024. DOI: 10.19080/CTBEB.2020.19.556024

Gupta S, Summuna B, Gupta M, Annepu SK. Edible mushrooms: cultivation, bioactive molecules, and health benefits. In: Mérillon JM, Ramawat KG, editors. Bioactive molecules in food. Springer International Publishing; 2018. pp. 1–33. DOI: 10.1007/978-3-319-54528-8_86-1

Badalyan SM, Barkhudaryan A, Rapior S. Recent progress in research on the pharmacological potential of mushrooms and prospects for their clinical application. In: Agrawal D, Dhanasekaran M, editors. Medicinal mushrooms. Singapore: Springer; 2019. DOI: 10.1007/978-981-13-6382-5_1

Deshmukh SK, Sridhar KR, Badalyan SM, editors. Fungal biotechnology: prospects and avenues. 1st edition. CRC Press; 2022. DOI: 10.1201/9781003248316

De Silva DD, Rapior S, Sudarman E, Stadler M, Xu J, Alias AS, et al. Bioactive metabolites from macrofungi: ethnopharmacology, biological activities and chemistry. Fungal Divers. 2013;62(1):1-40. DOI: 10.1007/s13225-013-0265-2

Hyde KD, Xu J, Rapior S, Jeewon R, Lumyong S, Niego AGT, et al. The amazing potential of fungi: 50 ways we can exploit fungi industrially. Fungal Divers. 2019;97(1):1-136. DOI: 10.1007/s13225-019-00430-9

Grienke U, Zöll M, Peintner U, Rollinger JM. European medicinal polypores--a modern view on traditional uses. J Ethnopharmacol. 2014 Jul 3;154(3):564-83. DOI: 10.1016/j.jep.2014.04.030

Girometta C. Antimicrobial properties of Fomitopsis officinalis in the light of its bioactive metabolites: a review. Mycology. 2018 Oct 25;10(1):32-9. DOI: 10.1080/21501203.2018.1536680

Fijałkowska A, Muszyńska B, Sułkowska-Ziaja K, Kała K, Pawlik A, Stefaniuk D, et al. Medicinal potential of mycelium and fruiting bodies of an arboreal mushroom Fomitopsis officinalis in therapy of lifestyle diseases. Sci Rep. 2020 Nov 18;10(1):20081. DOI: 10.1038/s41598-020-76899-1

Muszyńska B, Fijałkowska A, Sułkowska-Ziaja K, Włodarczyk A, Kaczmarczyk P, Nogaj E, et al. Fomitopsis officinalis: a species of arboreal mushroom with promising biological and medicinal properties. Chem Biodivers. 2020 Jun;17(6):e2000213. DOI: 10.1002/cbdv.202000213

Wu X, Yang J, Zhou L, Dong Y. New lanostane-type triterpenes from Fomes officinalis. Chem Pharm Bull (Tokyo). 2004 Nov;52(11):1375-7. DOI: 10.1248/cpb.52.1375

Wu X, Yang JS, Yan M. Four new triterpenes from fungus of Fomes officinalis. Chem Pharm Bull (Tokyo). 2009 Feb;57(2):195-7. DOI: 10.1248/cpb.57.195

Hwang CH, Jaki BU, Klein LL, Lankin DC, McAlpine JB, Napolitano JG, et al. Chlorinated coumarins from the polypore mushroom Fomitopsis officinalis and their activity against Mycobacterium tuberculosis. J Nat Prod. 2013 Oct 25;76(10):1916-22. DOI: 10.1021/np400497f

Feng W, Yang JS. A new drimane sesquiterpenoid and a new triterpene lactone from fungus of Fomes officinalis. J Asian Nat Prod Res. 2015;17(11):1065-72. DOI: 10.1080/10286020.2015.1054378

Sha AL. Effects of the Fomes officinalis flavonoids on anti-senile action in the aging model mice. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2016 Feb 8;32(2):121-3. DOI: 10.13459/j.cnki.cjap.2016.02.007

Han JX, Yuan T. Two new triterpenoid acids from Uighur medicine Fomes officinalis. Zhongguo Zhong Yao Za Zhi. 2017 Apr;42(7):1225-8. DOI: 10.19540/j.cnki.cjcmm.20170224.021

Naranmandakh S, Murata T, Odonbayar B, Suganuma K, Batkhuu J, Sasaki K. Lanostane triterpenoids from Fomitopsis officinalis and their trypanocidal activity. J Nat Med. 2018 Mar;72(2):523-9. DOI: 10.1007/s11418-018-1182-1

Golovchenko VV, Khramova DS, Shinen N, Jamsranjav G, Chizhov AO, Shashkov AS. Structure characterization of the mannofucogalactan isolated from fruit bodies of Quinine conk Fomitopsis officinalis. Carbohydr Polym. 2018 Nov 1;199:161-9. DOI: 10.1016/j.carbpol.2018.06.103

Vedenicheva NP, Al Maali GA, Mykhaylova OB, Lomberg ML, Bisko NA, Shcherbatiuk MM, et al. Endogenous cytokikins dynamics in mycelial biomass of basidiomycetes at different stages of cultivation. Int J Biochem Physiol, 2018;3(2):000122. DOI: 10.23880/ijbp-16000122

Vedenicheva NP, Al-Maali GA, Bisko NA, Shcherbatiuk MM, Lomberg ML, Mytropolska NY, et al. Comparative analysis of cytokinins in mycelial biomass of medicinal mushrooms. Int J Med Mushrooms. 2018;20(9):837-47. DOI: 10.1615/IntJMedMushrooms.2018027797

Ren L, Perera C, Hemar Y. Antitumor activity of mushroom polysaccharides: a review. Food Funct. 2012 Nov;3(11):1118-30. DOI: 10.1039/c2fo10279j

Vazirian M, Faridfar S, Eftekhari M. "Gharikon"/"Agharikon" a valuable medicinal mushroom in Iranian traditional medicine. Iran J Med Sci. 2016; May;41(3 Suppl):S34.

Vitak T, Yurkiv B, Wasser S, Nevo E, Sybirna N. Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus. World J Diabetes. 2017 May 15;8(5):187-201. DOI: 10.4239/wjd.v8.i5.187

Golembiovska OI, Galkin AY, Besarab AB. Development and validation of a dissolution test for ursodeoxycholic acid and taurine from combined formulation. Sci Study Res Chem Chem Eng Biotechnol Food Ind. 2019;20(3):377-94.

Elkhateeba WA, Dabaa GM, Elnahasa MO, Thomasb PW. Fomitopsis officinalis mushroom: ancient gold mine of functional components and biological activities for modern medicine. Egypt Pharm J. 2019;18(4):285-9. DOI: 10.4103/epj.epj_46_19

Mykchaylova O, Poyedіnok N. Antimicrobial activity of Fomitopsis officinalis (Vill.) Bondartsev & Singer in pure culture. Innov Biosyst Bioeng. 2021;5(4):220-7. DOI: 10.20535/ibb.2021.5.4.246668

Panter F, Bader CD, Müller R. Synergizing the potential of bacterial genomics and metabolomics to find novel antibiotics. Chem Sci. 2021;12(17):5994-6010. DOI: 10.1039/d0sc06919a

Bisko N, Lomberg M, Mykchaylova O, Mytropolska N. IBK Mushroom Culture Collection. Version 1.2. The IBK Mu-shroom Culture Collection of the M.G. Kholodny Institute of Botany [Data file]. M.G. Kholodny Institute of Botany; 2020 [cited 2021 Apr 15]. DOI: 10.15468/dzdsqu

Bilay VI, editor. Methods of experimental mycology. Kyiv: Naukova Dumka; 1982. 550 p.

Bisko NA, Babitskaya VG, Bukhalo AS, Krupoderova TA, Lomberg ML, Mykhaylova OB, et al. Biological features of medicinal macromycetes in culture. Vol. 2. Kyiv: Altpres; 2012. 459 p.

Buchalo АS. Higher edible Basidiomycetes in pure culture. Kyiv: Naukova Dumka; 1988. 144 p.

Mykchaylova O, Lomberg M, Krasinko V. Biotechnological basis of intensive cultivation of medicinal mushroom Fomitopsis betulina (Fomitopsidaceae, Polyporales). Sci Works NUFT. 2021;27(1):32-41. DOI: 10.24263/2225-2924-2021-27-1-5

Poyedinok NL, Tugay TI, Tugay AV, Mykchaylova OB, Sergiichuk NN, Negriyko AM. Influence of nitrogen concentration on photoinduced growth, enzymatic activity and melanine synthesis by Inonotus obliquus (Ach.: Pers.) Pilát. Biotechnol Acta. 2019;12(4):34-41. DOI: 10.15407/biotech12.04.034

Bisko N, Mustafin K, Al-Maali GA, Suleimenova Z, Lomberg M, Narmuratova Z, et al. Effects of cultivation parameters on intracellular polysaccharide production in submerged culture of the edible medicinal mushroom Lentinula edodes. Czech Mycol. 2020;72(1):1-17. DOI: 10.33585/cmy.72101

Piętka J. The development of Fomitopsis officinalis mycelium grown on organic media and larch wood under laboratory conditions. Sylwan. 2004;9:34-42.

Motronenko V, Lutsenko T, Galkin A, Gorshunov Y, Solovjova V. Optimization of the culture medium composition to increase the biosynthesis of recombinant human interleukin-7 in Escherichia coli. J Microbiol, Biotechnol Food Sci. 2020;9(4):761-8. DOI: 10.15414/jmbfs.2020.9.4.761-768

Fijałkowska A, Krakowska A, Lazur J, Włodarczyk A, Zięba P, Suchanek M, et al. Fortified mycelium of Fomitopsis officinalis (Agaricomycetes) as a source of biologically active substances effective in the prevention of civilization diseases. Int J Med Mushrooms. 2021;23(9):29-44. DOI: 10.1615/IntJMedMushrooms.2021039778

Gromovykh TI, Kovalyeva GK. Biological features and productivity of a new strain of Fomitopsis officinalis. Bull Krasnoyarsk State Agrar University. 2009;1:68-75.

Sidorenko ML, Buzoleva LS. Search for new types of raw materials for antibacterial drugs. Antibiot Khimioter. 2012;57(5-6):7-10.

Gromovykh TI, Gavryushina IA, Sadykova VS, Feldman NB, Dmitrenok AS, Ayrapetova AYu, et al. Obtaining immobilized mycelium of basidiomycete Fomitopsis officinalis (Vill.:Fr.) bond. et Sing., producer of agaricic acid. Antibiot Khimioter. 2018;63(9-10):3-10.

Mykhaylova OB. Morphological and cultural properties of a medicinal mushroom, Piptoporus betulinus (Basidiomycetes), on nutrient agar media. Ukr Botan J. 2014;71(5):603-9. DOI: 10.15407/ukrbotj71.05.603

Liu X, Zhao M, Wang Q. Biological characteristics of five wood-rotting fungi and wood-decaying ability to Betula platyphylla. Front For China. 2009;4(4):508. DOI: 10.1007/s11461-009-0073-8

Choi DB, Maeng JM, Ding JL, Cha WS. Exopolysaccharide production and mycelial growth in an air-lift bioreactor using Fomitopsis pinicola. J Microbiol Biotechnol. 2007;17(8):1369-78.

Mykchaylova ОB, Bisko NА, Sukhomlyn ММ, Lomberg МL, Pasaylyuk МV, Petrichuk YV, et al. Biological peculiarities of a rare medicinal mushroom Fomitopsis officinalis (Fomitopsidaceae, Polyporales) on agar media and plant substrates. Regulat Mech Biosyst. 2017;4(8):469-75. DOI: 10.15421/021772

Published

2023-02-17

How to Cite

1.
Mykchaylova O, Poyedinok N, Shchetinin V. Screening of Strains of the Medicinal Mushroom Fomitopsis officinalis (vill.) Bondartsev & Singer Promising for Biotechnological Use. Innov Biosyst Bioeng [Internet]. 2023Feb.17 [cited 2024Mar.29];6(3-4):110-8. Available from: http://ibb.kpi.ua/article/view/271383

Issue

Section

Articles