https://ibb.kpi.ua/ <p>The scientific journal <em>Innovative Biosystems and Bioengineering</em> was founded in 2017. IBB introduces a systems approach to life sciences problems.</p> <p>IBB is a quarterly peer-reviewed Open Access e-journal in which readers, immediately upon online publication, can access articles free of costs and subscription charges.</p> <p>e-ISSN 2616-177X</p> <p>Founder and Publisher: National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”.</p> <p>Frequency: 4 issues a year.</p> <p>We accept papers in the following language: English.</p> <p>Cite the title as: Innov Biosyst Bioeng.</p> <p>Readership: Biotechnologists, Bioengineers, Biomedical researchers and engineers, Biologists.</p> <p>Indexing: Scopus; DOAJ; ROAD; HINARI; Chemical Abstracts Service; CNKI Scholar; Norwegian Register for Scientific Journals, Series and Publishers; J-Gate; Public Knowledge Project Index; ICMJE; JournalTOCs; WCOSJ; Vifabio; EZB; Federation of Finnish Learned Societies; Zeitschriftendatenbank; Polska Bibliografia Naukowa; Scilit; Bielefeld Academic Search Engine; OpenAir; WorldCat.</p> Igor Sikorsky Kyiv Polytechnic Institute en-US Innovative Biosystems and Bioengineering 2616-177X <p><span>The ownership of copyright remains with the Authors.</span></p><p>Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.</p><p>Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.</p>IBB articles are published under Creative Commons licence:<br /><ol type="a"><li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under <a href="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.<br /><br /></li><li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.<br /><br /></li><li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.</li></ol> https://ibb.kpi.ua/article/view/343389 <p><strong>Rationale. </strong>Long-term storage of erythrocytes, a vital component of modern transfusion medicine, is accompanied by structural, metabolic, and biophysical alterations that reduce their functional activity. Differentiating erythrocytes by surface charge represents a promising approach for assessing viability, predicting post-transfusion behavior, and optimizing storage and biobanking strategies. However, the practical application of zeta potential analysis in clinical transfusion medicine remains insufficiently explored.</p> <p><strong>Aim.</strong> To summarize current knowledge on erythrocyte heterogeneity by surface charge, their biophysical properties, diagnostic value, and application prospects in transfusion medicine and biobanking.</p> <p><strong>Methods.</strong> A systematic literature search was conducted using PubMed, Scopus, Web of Science, Cochrane Library, Google Scholar, and Clinical Key, focusing on studies addressing erythrocyte biophysics, surface charge parameters, population heterogeneity, zeta potential measurement methods, and clinical applications.</p> <p><strong>Results.</strong> Erythrocyte heterogeneity by surface charge is a fundamental population property shaped by physiological aging, oxidative stress, and membrane–cytoskeleton modifications. Reduced zeta potential is associated with enhanced aggregation, impaired deformability, and decreased microvascular passage. Analysis of zeta potential enables identification of subpopulations with varying levels of damage and prediction of their functional performance after transfusion. In transfusion medicine, this approach may improve transfusion efficiency and safety, while in biobanking it offers opportunities for better selection of cells for long-term storage. Surface charge differentiation also holds promise for predicting erythrocyte shelf life and advancing personalized transfusion strategies.</p> <p><strong>Conclusions.</strong> Integration of zeta potential analysis into laboratory practice could enhance the quality of blood components and support the development of personalized transfusion medicine.</p> Daniil Liadov Volodymyr Berest Tetiana Liadova Fedir Hladkykh Copyright (c) 2025 The Author(s) http://creativecommons.org/licenses/by/4.0 2025-11-24 2025-11-24 9 4 3 15 10.20535/ibb.2025.9.4.343389