https://ibb.kpi.ua/ <p>The scientific journal <em>Innovative Biosystems and Bioengineering</em> was founded in 2017. IBB introduces a systems approach to life sciences problems.</p> <p>IBB is a quarterly peer-reviewed Open Access e-journal in which readers, immediately upon online publication, can access articles free of costs and subscription charges.</p> <p>e-ISSN 2616-177X</p> <p>Founder and Publisher: National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”.</p> <p>Frequency: 4 issues a year.</p> <p>We accept papers in the following language: English.</p> <p>Cite the title as: Innov Biosyst Bioeng.</p> <p>Readership: Biotechnologists, Bioengineers, Biomedical researchers and engineers, Biologists.</p> <p>Indexing: Scopus; DOAJ; ROAD; HINARI; Chemical Abstracts Service; CNKI Scholar; Norwegian Register for Scientific Journals, Series and Publishers; J-Gate; Public Knowledge Project Index; ICMJE; JournalTOCs; WCOSJ; Vifabio; EZB; Federation of Finnish Learned Societies; Zeitschriftendatenbank; Polska Bibliografia Naukowa; Scilit; Bielefeld Academic Search Engine; OpenAir; WorldCat.</p> Igor Sikorsky Kyiv Polytechnic Institute en-US Innovative Biosystems and Bioengineering 2616-177X <p><span>The ownership of copyright remains with the Authors.</span></p><p>Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.</p><p>Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.</p>IBB articles are published under Creative Commons licence:<br /><ol type="a"><li>Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under <a href="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0</a> that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.<br /><br /></li><li>Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.<br /><br /></li><li>Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.</li></ol> https://ibb.kpi.ua/article/view/322167 <p><strong>Background. </strong>The stages of wound healing following surgery are generally consistent, but immune responses and increased inflammation can delay the normal healing process. As a result, additional support is crucial. Perfusion plays an important role in accelerating skin wound healing, and the saliva of medicinal leeches has been shown to enhance this process.</p> <p><strong>Objective. </strong>To evaluate the effect of the medicinal leech <em>Hirudo verbana</em> on the morphological changes in an excisional wound.</p> <p><strong>Methods. </strong>An excisional wound was created in the interscapular region of animals in both the control and experimental groups. In the experimental groups, one medicinal leech was applied on days 1, 3, 7, and 14. Tissue samples from the wound edge were collected immediately after leech application, and then at days 3, 7, 14, and 30 during the healing process. These samples were processed using standard histological techniques.</p> <p><strong>Results. </strong>In the experimental group, intensive formation of granulation tissue was observed as early as day 3, compared to the control group. An increase in the thickness of the papillary layer, which was vascularized and supported epidermal nutrition, was noted in the experimental group at almost all time points. This may have contributed to enhanced proliferation processes, an increase in the number of hair follicles and sebaceous glands, and a reduction in scar size. On day 3, the number of leukocytes decreased, signaling a reduction in inflammation compared to the control group. By day 7, a significant reduction in subcutaneous tissue and the areas of hair follicles and sebaceous glands was observed, suggesting an increase in basal metabolic activity.</p> <p><strong>Conclusions.</strong> The complex of biologically active substances from the medicinal leech <em>Hirudo verbana</em> positively affects the processes of reparative regeneration in excisional wounds, accelerating all stages of wound healing. It significantly increases the number of newly formed hair follicles and blood vessels, directly indicating the regenerative properties of these substances.</p> Ruslan Aminov Alina Aminova Copyright (c) 2025 The Author(s) http://creativecommons.org/licenses/by/4.0 2025-06-06 2025-06-06 9 3 3 13 10.20535/ibb.2025.9.3.322167 https://ibb.kpi.ua/article/view/306265 <p><strong>Background.</strong> Modifying the metabolism of microalgae through chemical and physical environmental factors to obtain useful substances.</p> <p><strong>Objective.</strong> To summarize literature data on the effects of elevated concentrations of heavy metal ions on the biosynthesis of carotenoids, chlorophylls, and unsaturated fatty acids in microalgae, and to analyze the influence of lighting regimes as well as ultrasonic, ultraviolet, and gamma irradiation on the performance of various microalgae species.</p> <p><strong>Methods.</strong> A review and synthesis of literature data on the impact of increased heavy metal ion concentrations, lighting conditions, and exposure to ultrasound, UV, and gamma radiation on both the performance of different microalgae species and the biosynthesis of carotenoids, chlorophylls, and unsaturated fatty acids.</p> <p><strong>Results.</strong> The influence of physical and chemical environmental factors on nutrient biosynthesis in microalgae is species-specific. Elevated metal ion concentrations may either stimulate or inhibit the biosynthesis of various metabolites, including lipids, carotenoids, chlorophylls, proteins, and carbohydrates. Variations in light spectrum and intensity, as well as the frequency and duration of exposure to ultrasound, UV, and gamma radiation, can alter the metabolic pathways of microalgae in a species-dependent manner.</p> <p><strong>Conclusions.</strong> The metabolism of microalgae is influenced by cultivation parameters, species type, and the composition of the growth environment. Optimizing microalgae cultivation by adjusting physical and chemical abiotic factors for enhanced nutrient production requires a species-specific approach. The presented analysis forms a foundation for further research and the development of technological solutions aimed at boosting the biosynthesis of valuable compounds in microalgae.</p> Nataliia Golub Svitlana Kovalova Copyright (c) 2025 The Author(s) http://creativecommons.org/licenses/by/4.0 2025-06-13 2025-06-13 9 3 14 32 10.20535/ibb.2025.9.3.306265