Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content

Authors

  • Taisa Bohdanovych Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Ukraine
  • Nadiia Matvieieva Institute of Cell Biology and Genetic Engineering, NAS of Ukraine, Ukraine

DOI:

https://doi.org/10.20535/ibb.2022.6.3-4.271259

Keywords:

silver nanoparticles, "hairy" roots, Artemisia tilesii Ledeb., flavonoids, reducing power

Abstract

Background. "Hairy" roots can be used for production of plant-derived secondary metabolites, such as flavonoids, which have antioxidant and reducing properties. It seems promising to use the process of silver nanoparticles formation as a method of determining the level of reducing power. This approach allows simul­taneously to obtain nanoparticles with different biological activity and evaluate the reducing potential of dif­ferent plants.

Objective. The aim of the study was to determine the dependence of optical properties (ultraviolet/visible spectroscopy, UV-Vis spectra) of solutions of silver nanoparticles obtained using wormwood "hairy" root extracts on the content of flavonoids as compounds with reducing activity.

Methods. Four Artemisia tilesii “hairy” root lines from the collection of the Institute of Cell Biology and Genetic Engineering NAS of Ukraine were grown for five weeks in liquid 1/2 MS medium with 20 g/l sucrose. "Hairy" roots were homogenized in 70% ethanol, the extracts were centrifuged, total flavonoid content was determined, and extracts were used for the silver nanoparticles preparation via reduction of Ag+ to Ag0 from AgNO3. UV-Vis spectra at 300–600 nm range were recorded right after colloid solution preparation, in five, and in nine days.

Results. Total flavonoid content varied from 4.01 ± 0.39 to 15.37 ± 1.08 mg RE/g FW. The UV-Vis spectra curves of absorption increased with the course of time, mostly from day 0 to day 5. At this period, absorption at 370–500 nm wavelength increased almost twofold. The peak absorption of all samples was detected at 440 nm, and the maximum values at the wavelength of 440 nm correlated with the content of flavonoids. This correlation did not change over time.

Conclusions. The optical properties of silver nanoparticles colloid solutions obtained using A. tilesii "hairy" root extracts correlated with the total flavonoid content of the samples. Even though the absorbance of the colloid solutions increased with time, those increases correlated with flavonoid content as well. The determination of the optical properties of AgNPs colloid solution can be used as a convenient way of quickly comparing the reducing ability of extracts both right after the formation of a colloidal solution and after some time of its storage.

References

Piatczak E, Dfbska M, Kontek B, Olas B, Wysokinskai H. Antioxidant properties of methanolic extracts from the shoots and roots of pRi-transformed plants of Rehmannia glutinosa Libosch. Acta Pol Pharm. 2016;73(2):433-8.

El-Esawi MA, Elkelish A, Elansary HO, Ali HM, Elshikh M, Witczak J, et al. Genetic transformation and hairy root induction enhance the antioxidant potential of Lactuca serriola L. Oxid Med Cell Longev. 2017;2017:1-8. DOI: 10.1155/2017/5604746

Gai Q-Y, Jiao J, Luo M, Wei Z-F, Zu Y-G, Ma W, et al. Establishment of hairy root cultures by agrobacterium rhizogenes mediated transformation of Isatis tinctoria L. for the efficient production of flavonoids and evaluation of antioxidant activities. PLoS One. 2015;10(3):e0119022. DOI: 10.1371/journal.pone.0119022

Zafari S, Sharifi M, Chashmi NA. A comparative study of biotechnological approaches for producing valuable flavonoids in Prosopis farcta. Cytotechnology. 2018;70(2):603-14. DOI: 10.1007/s10616-017-0143-y

Tavassoli P, Safipour Afshar A. Influence of different agrobacterium rhizogenes strains on hairy root induction and analysis of phenolic and flavonoid compounds in marshmallow (Althaea officinalis L.). 3 Biotech. 2018;8(8):351. DOI: 10.1007/s13205-018-1375-z

Mohaddab M, El Goumi Y, Gallo M, Montesano D, Zengin G, Bouyahya A, et al. Biotechnology and in vitro culture as an alternative system for secondary metabolite production. Molecules. 2022;27(22):8093. DOI: 10.3390/molecules27228093

Kowalczyk T, Wieczfinska J, Skała E, Śliwiński T, Sitarek P. Transgenesis as a tool for the efficient production of selected secondary metabolites from plant in vitro cultures. Plants. 2020;9(2):132. DOI: 10.3390/plants9020132

Sathasivam R, Choi M, Radhakrishnan R, Kwon H, Yoon J, Yang SH, et al. Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum. Front Plant Sci. 2022;13:983776. DOI: 10.3389/fpls.2022.983776

Morey KJ, Peebles CAM. Hairy roots: An untapped potential for production of plant products. Front Plant Sci. 2022;13:937095. DOI: 10.3389/fpls.2022.937095

Sharma S, Vig AP. Evaluation of in vitro antioxidant properties of methanol and aqueous extracts of Parkinsonia aculeata L. leaves. ScientificWorldJournal. 2013;2013:604865. DOI: 10.1155/2013/604865

Wong FC, Yong AL, Ting EP, Khoo SC, Ong HC, Chai TT. Antioxidant, metal chelating, anti-glucosidase activities and phytochemical analysis of selected tropical medicinal plants. Iran J Pharm Res. 2014;13(4):1409-15.

Shahid M, Law D, Azfaralariff A, Mackeen MM, Chong TF, Fazry S. Phytochemicals and biological activities of Garcinia atroviridis: A critical review. Toxics. 2022;10(11):656. DOI: 10.3390/toxics10110656

Mbida H, Tsala DE, Aboubakar S, Habtemariam S, Edmond JJ, Bakwo EF, et al. Antioxidant activity of aqueous extract of leaves and seeds of Datura metel (Solanaceae) in frog's heart failure model. Evid Based Complement Alternat Med. 2022;2022:5318117. DOI: 10.1155/2022/5318117

Klongdee S, Klinkesorn U. Optimization of accelerated aqueous ethanol extraction to obtain a polyphenol-rich crude extract from rambutan (Nephelium lappaceum L.) peel as natural antioxidant. Sci Rep. 2022;12(1):21153. DOI: 10.1038/s41598-022-25818-7

Szabo K, Ranga F, Elemer S, Varvara RA, Diaconeasa Z, Dulf FV, et al. Evaluation of the Astragalus exscapus L. subsp. transsilvanicus roots' chemical profile, phenolic composition and biological activities. Int J Mol Sci. 2022;23(23):15161. DOI: 10.3390/ijms232315161

Kebert M, Kostić S, Vuksanović V, Gavranović Markić A, Kiprovski B, Zorić M, et al. Metal- and organ-specific response to heavy metal-induced stress mediated by antioxidant enzymes' activities, polyamines, and plant hormones levels in Populus deltoides. Plants (Basel). 2022;11(23):3246. DOI: 10.3390/plants11233246

Mandal D, Sarkar T, Chakraborty R. Critical review on nutritional, bioactive, and medicinal potential of spices and herbs and their application in food fortification and nanotechnology. Appl Biochem Biotechnol. 2023;195(2):1319-513. DOI: 10.1007/s12010-022-04132-y

Abdelhameed RFA, Nafie MS, Hal DM, Nasr AM, Swidan SA, Abdel-Kader MS, et al. Comparative cytotoxic evaluation of Zygophyllum album root and aerial parts of different extracts and their biosynthesized silver nanoparticles on lung A549 and prostate PC-3 cancer cell lines. Pharmaceuticals (Basel). 2022;15(11):1334. DOI: 10.3390/ph15111334

Wei Z, Xu S, Jia H, Zhang H. Green synthesis of silver nanoparticles from Mahonia fortunei extracts and characterization of its inhibitory effect on Chinese cabbage soft rot pathogen. Front Microbiol. 2022;13:1030261. DOI: 10.3389/fmicb.2022.1030261

Yousaf A, Waseem M, Javed A, Baig S, Ismail B, Baig A, et al. Augmented anticancer effect and antibacterial activity of silver nanoparticles synthesized by using Taxus wallichiana leaf extract. PeerJ. 2022;10:e14391. DOI: 10.7717/peerj.14391

Mostafa Abdalhamed A, Zeedan GSG, Ahmed Arafa A, Shafeek Ibrahim E, Sedky D, Abdel Nabey Hafez A. Detection of methicillin-resistant Staphylococcus aureus in clinical and subclinical mastitis in ruminants and studying the effect of novel green synthetized nanoparticles as one of the alternative treatments. Vet Med Int. 2022;2022:6309984. DOI: 10.1155/2022/6309984

Balciunaitiene A, Puzeryte V, Radenkovs V, Krasnova I, Memvanga PB, Viskelis P, et al. Sustainable-green synthesis of silver nanoparticles using aqueous Hyssopus officinalis and Calendula officinalis extracts and their antioxidant and antibacterial activities. Molecules. 2022;27(22):7700. DOI: 10.3390/molecules27227700

Fierascu IC, Fierascu I, Baroi AM, Ungureanu C, Ortan A, Avramescu SM, et al. Phytosynthesis of biological active silver nanoparticles using Echinacea purpurea L. extracts. Materials (Basel). 2022;15(20):7327. DOI: 10.3390/ma15207327

Alsareii SA, Manaa Alamri A, AlAsmari MY, Bawahab MA, Mahnashi MH, Shaikh IA, et al. Synthesis and characterization of silver nanoparticles from Rhizophora apiculata and studies on their wound healing, antioxidant, anti-inflammatory, and cytotoxic activity. Molecules. 2022;27(19):6306. DOI: 10.3390/molecules27196306

Ahmed SS, Alqahtani AM, Alqahtani T, Alamri AH, Menaa F, Mani RK, et al. Green synthesis, characterizations of zinc oxide nanoparticles from aqueous leaf extract of Tridax procumbens Linn. and Assessment of their anti-hyperglycemic activity in streptozoticin-induced diabetic rats. Materials (Basel). 2022;15(22):8202. DOI: 10.3390/ma15228202

Adu OT, Mohamed F, Naidoo Y, Adu TS, Chenia H, Dewir YH, et al. Green synthesis of silver nanoparticles from Diospyros villosa extracts and evaluation of antioxidant, antimicrobial and anti-quorum sensing potential. Plants (Basel). 2022;11(19):2514. DOI: 10.3390/plants11192514

Naiel B, Fawzy M, Halmy MWA, Mahmoud AED. Green synthesis of zinc oxide nanoparticles using Sea Lavender (Limonium pruinosum L. Chaz.) extract: characterization, evaluation of anti-skin cancer, antimicrobial and antioxidant potentials. Sci Rep. 2022;12(1):20370. DOI: 10.1038/s41598-022-24805-2

Dehghani F, Mosleh-Shirazi S, Shafiee M, Kasaee SR, Amani AM. Antiviral and antioxidant properties of green synthesized gold nanoparticles using Glaucium flavum leaf extract. Appl Nanosci. 2022;1-11. DOI: 10.1007/s13204-022-02705-1

Sharma S, Kumari P, Thakur P, Brar GS, Bouqellah NA, Hesham AE. Synthesis and characterization of Ni0.5Al0.5Fe2O4 nanoparticles for potent antifungal activity against dry rot of ginger (Fusarium oxysporum). Sci Rep. 2022;12(1):20092. DOI: 10.1038/s41598-022-22620-3

Mikhailova EO. Green synthesis of platinum nanoparticles for biomedical applications. J Funct Biomater. 2022;13(4):260. DOI: 10.3390/jfb13040260

Tymoszuk A, Sławkowska N, Szałaj U, Kulus D, Antkowiak M, Wojnarowicz J. Synthesis, characteristics, and effect of zinc oxide and silver nanoparticles on the in vitro regeneration and biochemical profile of Chrysanthemum adventitious shoots. Materials (Basel). 2022;15(22):8192. DOI: 10.3390/ma15228192

Sampath G, Chen YY, Rameshkumar N, Krishnan M, Nagarajan K, Shyu DJH. Biologically synthesized silver nanoparticles and their diverse applications. Nanomaterials (Basel). 2022;12(18):3126. DOI: 10.3390/nano12183126

Alim MA, Bayazid G, Rahman R, Bosu R, Shamma S. Silver nanoparticle synthesis, UV-Vis spectroscopy to find particle size and measure resistance of colloidal solution. J Phys Conf Ser. 2020;1706(1):012020. DOI: 10.1088/1742-6596/1706/1/012020

Matvieieva NA, Shakhovsky AM, Belokurova VB, Drobot KO. Artemisia tilesii Ledeb hairy roots establishment using Agrobacterium rhizogenes-mediated transformation. Prep Biochem Biotechnol. 2016;46(4):342-5. DOI: 10.1080/10826068.2015.1031393

Pekal A, Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal Methods. 2014;7(9):1776-82. DOI: 10.1007/s12161-014-9814-x

Kobylinska N, Shakhovsky A, Khainakova O, Klymchuk D, Avdeeva L, Ratushnyak Y, et al. "Hairy" root extracts as source for "green" synthesis of silver nanoparticles and medical applications. RSC Adv. 2020;10(65):39434-46. DOI: 10.1039/d0ra07784d

Zhu C, Sanahuja G, Yuan D, Farré G, Arjó G, Berman J, et al. Biofortification of plants with altered antioxidant content and composition: genetic engineering strategies. Plant Biotechnol J. 2013;11(2):129-41. DOI: 10.1111/j.1467-7652.2012.00740.x

Srivastava S, Srivastava AK. Hairy root culture for mass-production of high-value secondary metabolites. Crit Rev Biotechnol. 2007;27(1):29-43. DOI: 10.1080/07388550601173918

Ono NN, Tian L. The multiplicity of hairy root cultures: Prolific possibilities. Plant Sci. 2011;180(3):439-46. DOI: 10.1016/j.plantsci.2010.11.012

Kobylinska N, Bohdanovych T, Duplij V, Pashchenko I, Matvieieva N. Simultaneous identification, quantification, and analysis of main components of "hairy" root extracts of Artemisia annua and Artemisia tilesii Plants. Biotechnol Acta. 2021;14(6):60-70. DOI: 10.15407/biotech14.06.060

He L, He T, Farrar S, Ji L, Liu T, Ma X. Antioxidants maintain cellular redox homeostasis by elimination of reactive oxygen species. Cell Physiol Biochem. 2017;44(2):532-53. DOI: 10.1159/000485089

Olszowy M. What is responsible for antioxidant properties of polyphenolic compounds from plants? Plant Physiol Biochem. 2019;144:135-43. DOI: 10.1016/j.plaphy.2019.09.039

Albaqami JJ, Benny TP, Hamdi H, Altemimi AB, Kuttithodi AM, Job JT, et al. Phytochemical composition and in vitro antioxidant, anti-inflammatory, anticancer, and enzyme-inhibitory activities of Artemisia nilagirica (C.B. Clarke) Pamp. Molecules. 2022;27(20):7119. DOI: 10.3390/molecules27207119

Mishra S, Pandey A, Manvati S. Coumarin: An emerging antiviral agent. Heliyon. 2020;6(1):e03217. DOI: 10.1016/j.heliyon.2020.e03217

Enogieru AB, Haylett W, Hiss DC, Bardien S, Ekpo OE. Rutin as a potent antioxidant: implications for neurodegenerative disorders. Oxid Med Cell Longev. 2018;2018:6241017. DOI: 10.1155/2018/6241017

Al-Dhabi NA, Arasu MV, Park CH, Park SU. An up-to-date review of rutin and its biological and pharmacological activities. EXCLI J. 2015;14:59-63. DOI: 10.17179/excli2014-663

Rashmi HB, Negi PS. Phenolic acids from vegetables: A review on processing stability and health benefits. Food Res Int. 2020;136:109298. DOI: 10.1016/j.foodres.2020.109298

Kshirsagar SG, Rao RV. Antiviral and immunomodulation effects of Artemisia. Medicina. 2021;57(3):217. DOI: 10.3390/medicina57030217

Malik S, de Mesquita L, Silva C, de Mesquita J, de Sá Rocha E, Bose J, et al. Chemical profile and biological activities of essential oil from Artemisia vulgaris L. cultivated in Brazil. Pharmaceuticals. 2019;12(2):49. DOI: 10.3390/ph12020049

Trendafilova A, Moujir LM, Sousa PM, Seca AM. Research advances on health effects of edible Artemisia species and some sesquiterpene lactones constituents. Foods. 2020;10(1):65. DOI: 10.3390/foods10010065

Lianza M, Mandrone M, Chiocchio I, Tomasi P, Marincich L, Poli F. Screening of ninety herbal products of commercial interest as potential ingredients for phytocosmetics. J Enzyme Inhib Med Chem. 2020;35(1):1287-91. DOI: 10.1080/14756366.2020.1774571

Mazumder K, Biswas B, Raja IM, Fukase K. A review of cytotoxic plants of the indian subcontinent and a broad-spectrum analysis of their bioactive compounds. Molecules. 2020;25(8):1904. DOI: 10.3390/molecules25081904

Rolnik A, Olas B. The plants of the Asteraceae family as agents in the protection of human health. Int J Mol Sci. 2021;22(6):3009. DOI: 10.3390/ijms22063009

Saarela JM, Sokoloff PC, Gillespie LJ, Bull RD, Bennett BA, Ponomarenko S. Vascular plants of Victoria Island (Northwest Territories and Nunavut, Canada): a specimen-based study of an Arctic flora. PhytoKeys. 2020;141:1-330. DOI: 10.3897/phytokeys.141.48810

Native American Ethnobotany Database [Internet]. Naeb.brit.org. 2022 [Cited 2022 Nov 4]. Available: http://naeb.brit.org/uses/search/?string=artemisia+tilesii/

Viršilė A, Samuolienė G, Laužikė K, Šipailaitė E, Balion Z, Jekabsone A. Species-specific plant-derived nanoparticle characteristics. Plants (Basel). 2022;11(22):3139. DOI: 10.3390/plants11223139

Bordoni V, Sanna L, Lyu W, Avitabile E, Zoroddu S, Medici S, et al. Silver nanoparticles derived by Artemisia arborescens reveal anticancer and apoptosis-inducing effects. Int J Mol Sci. 2021;22(16):8621. DOI: 10.3390/ijms22168621

Rasheed T, Bilal M, Iqbal HMN, Li C. Green biosynthesis of silver nanoparticles using leaves extract of Artemisia vulgaris and their potential biomedical applications. Colloids Surf B Biointerfaces. 2017;158:408-15. DOI: 10.1016/j.colsurfb.2017.07.020

Mousavi B, Tafvizi F, Zaker Bostanabad S. Green synthesis of silver nanoparticles using Artemisia turcomanica leaf extract and the study of anti-cancer effect and apoptosis induction on gastric cancer cell line (AGS). Artif Cells Nanomed Biotechnol. 2018;46(sup1):499-510. DOI: 10.1080/21691401.2018.1430697

Ali EM, Abdallah BM. Effective inhibition of invasive pulmonary aspergillosis by silver nanoparticles biosynthesized with Artemisia sieberi leaf extract. Nanomaterials (Basel). 2021;12(1):51. DOI: 10.3390/nano12010051

Elemike EE, Onwudiwe DC, Ekennia AC, Jordaan A. Synthesis and characterisation of silver nanoparticles using leaf extract of Artemisia afra and their in vitro antimicrobial and antioxidant activities. IET Nanobiotechnol. 2018;12(6):722-6. DOI: 10.1049/iet-nbt.2017.0297

Park Y, Noh HJ, Han L, Kim HS, Kim YJ, Choi JS, et al. Artemisia capillaris extracts as a green factory for the synthesis of silver nanoparticles with antibacterial activities. J Nanosci Nanotechnol. 2012;12(9):7087-95. DOI: 10.1166/jnn.2012.6575

Moulavi P, Noorbazargan H, Dolatabadi A, Foroohimanjili F, Tavakoli Z, Mirzazadeh S, Hashemi M, Ashrafi F. Antibiofilm effect of green engineered silver nanoparticles fabricated from Artemisia scoporia extract on the expression of icaA and icaR genes against multidrug-resistant Staphylococcus aureus. J Basic Microbiol. 2019;59(7):701-12. DOI: 10.1002/jobm.201900096

Salehi S, Shandiz SA, Ghanbar F, Darvish MR, Ardestani MS, Mirzaie A, et al. Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties. Int J Nanomedicine. 2016;11:1835-46. DOI: 10.2147/IJN.S99882

Alavi M, Karimi N. Characterization, antibacterial, total antioxidant, scavenging, reducing power and ion chelating activities of green synthesized silver, copper and titanium dioxide nanoparticles using Artemisia haussknechtii leaf extract. Artif Cells Nanomed Biotechnol. 2018;46(8):2066-81. DOI: 10.1080/21691401.2017.1408121

Aghajanyan A, Gabrielyan L, Schubert R, Trchounian A. Silver ion bioreduction in nanoparticles using Artemisia annua L. extract: characterization and application as antibacterial agents. AMB Express. 2020;10(1):66. DOI: 10.1186/s13568-020-01002-w

Bohdanovych TA, Shakhovsky AM, Duplij VP, Ratushnyak Y, Kuchuk M, Poyedinok N, et al. Effects of genetic transformation on the antioxidant activity of “hairy” roots of Althaea officinalis L., Artemisia vulgaris L., and Artemisia tilesii Ledeb. Cytol Genet. 2021;55(6):531-9. DOI: 10.3103/S0095452721060037

Dutta MS, Mahapatra P, Ghosh A, Basu S. Estimation of the reducing power and electrochemical behavior of few flavonoids and polyhydroxybenzophenones substantiated by bond dissociation energy: a comparative analysis. Mol Divers. 2022;26(2):1101-13. DOI: 10.1007/s11030-021-10232-4

Wu H, Wang Y, Huang J, Li Y, Lin Z, Zhang B. Rutin ameliorates gout via reducing XOD activity, inhibiting ROS production and NLRP3 inflammasome activation in quail. Biomed Pharmacother. 2022;158:114175. DOI: 10.1016/j.biopha.2022.114175

Zheng YZ, Chen DF, Deng G, Guo R. the substituent effect on the radical scavenging activity of apigenin. Molecules. 2018;23(8):1989. DOI: 10.3390/molecules23081989

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2023-03-19

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Bohdanovych T, Matvieieva N. Optical Characteristics of Silver Nanoparticles Obtained Using Artemisia tilesii Ledeb. "Hairy" Root Extracts With High Flavonoid Content. Innov Biosyst Bioeng [Internet]. 2023Mar.19 [cited 2024Apr.21];6(3-4):169-77. Available from: http://ibb.kpi.ua/article/view/271259

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