Indicators of Oxidative Stress in Rat Brain Tissue Under Conditions of Prolonged Arterial Hypertension and Its Correction With Different Generations of Beta-blockers
DOI:
https://doi.org/10.20535/ibb.2026.10.1.359682Keywords:
arterial hypertension, beta-blockers, oxidative stress, hippocampusAbstract
Background. The significant increase in the number of patients with arterial hypertension both worldwide and in our country necessitates a more detailed study of the pathogenetic features of changes that occur in target organs, including the brain, in conditions of prolonged elevated blood pressure and against the background of its correction with beta-blockers of different generations.
Objective. The aim of the study was to examine oxidative stress indicators in brain homogenates of animals with arterial hypertension and to determine the effect of long-term correction with different generations of beta-blockers on the state of pro- and antioxidant components of OS.
Methods. One intact and four groups of experimental animals were studied. Experimental animals with spontaneous arterial hypertension were administered propranolol, carvedilol, and hypertril in therapeutic doses. The oxidative stress indicators (oxidative modification of proteins markers (aldehyde dinitrophenylhydrazones and carboxyphenylhydrazones) and content of reduced glutathione, and the activity of Superoxide dismutase, Glutathione peroxidase) in animal brain homogenates were biochemically studied.
Results. It was established that prolonged arterial hypertension is accompanied by a deterioration in the state of antioxidant systems. Correction of high blood pressure with propranolol does not significantly affect the state of oxidative stress indicators. Carvedilol has a moderate antioxidant effect, while hypertril has a powerful antioxidant effect, which is manifested in a decrease in oxidative modification of proteins markers, and increased of antioxidant system markers content.
Conclusions. Long-term administration of Propranolol stabilizes arterial pressure but does not alleviate oxidative stress in the brain, evidenced by sustained high levels of OMP markers (ADPH and CPH) and suppressed antioxidant defense (SOD, GSH, GPx). Carvedilol exerts a moderate antioxidant effect by lowering CPH levels but fails to significantly restore the activity of key antioxidant enzymes (SOD, GPx) or reduced glutathione levels. Hypertril exhibits a potent dual effect, providing both antihypertensive control and robust antioxidant neuroprotection. Its mechanism involves the significant reduction of OMP markers (ADPH and CPH) and the restoration of the antioxidant defense system (SOD, GSH, GPx).
References
Lysenko V. Oxidative stress as a non-specific factor of organ damage pathogenesis (review of literature and own data). Emergency Medicine. 2020;16(1):24–35. DOI: 10.22141/2224-0586.16.1.2020.196926
Egea G, Jiménez-Altayó F, Campuzano V. Reactive oxygen species and oxidative stress in the pathogenesis and progression of genetic diseases of the connective tissue. Antioxidants (Basel). 2020;9(10):1013. DOI: 10.3390/antiox9101013
Reddy VP. Oxidative stress in health and disease. Biomedicines. 2023;11(11):2925. DOI: 10.3390/biomedicines11112925
Jurcau MC, Jurcau A, Diaconu RG. Oxidative stress in the pathogenesis of neurodegenerative diseases. Stresses. 2024;4(4):827–849. DOI: 10.3390/stresses4040055
Dash UC, Bhol NK, Swain SK, Samal RR, Nayak PK, Raina V, et al. Oxidative stress and inflammation in the pathogenesis of neurological disorders: mechanisms and implications. Acta Pharm Sin B. 2025;15(1):15–34. DOI: 10.1016/j.apsb.2024.10.004
Kreutz R, et al. 2024 European Society of Hypertension clinical practice guidelines for the management of arterial hypertension. Eur J Intern Med. 2024. DOI: 10.1016/j.ejim.2024.05.033
Toyoda S, Haruyama A, Inami S, Arikawa T, Saito F, Watanabe R, et al. Effects of carvedilol vs bisoprolol on inflammation and oxidative stress in patients with chronic heart failure. J Cardiol. 2020;75(2):140–147. DOI: 10.1016/j.jjcc.2019.07.011
Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. Oxford: Clarendon Press; 1999. 320 p.
Hemmadi V. Estimation of superoxide dismutase. 2016. DOI: 10.13140/RG.2.1.2204.8407
Sattar AA, Matin AA, Hadwan MH, et al. Rapid and effective protocol to measure glutathione peroxidase activity. Bull Natl Res Cent. 2024;48:100. DOI: 10.1186/s42269-024-01250-x
Gok G, Neselioglu S, Ko HL, López-Martínez MJ, Manteca X, Rubio CP. Evaluation of glutathione (GSH) system in porcine saliva: validation and application of colorimetric method. Antioxidants. 2024;13(10):1231. DOI: 10.3390/antiox13101231
Mesa SM, Megerssa YC. Comparison of biuret and refractometry method for serum total protein measurements in cattle and goat. BMC Res Notes. 2024;17:234. DOI: 10.1186/s13104-024-06906-5
Svitlytskyi AO, Yehorov AA, Melnikova OV, Hrekova TA. Disorders of the central nervous system cognitive and mnestic functions in genetic arterial hypertension and with the use of beta-blockers of different generations. Modern Medical Technology. 2024;16(3):220–227. DOI: 10.14739/mmt.2024.3.310996
Erel Ö, Erdoğan S. Thiol-disulfide homeostasis: an integrated approach with biochemical and clinical aspects. Turk J Med Sci. 2020;50(SI-2):1728–1738. DOI: 10.3906/sag-2003-64
Belenichev I, Popazova O, Bukhtiyarova N, Ryzhenko V, Pavlov S, Suprun E, et al. Targeting mitochondrial dysfunction in cerebral ischemia: advances in pharmacological interventions. Antioxidants. 2025;14(1):108. DOI: 10.3390/antiox14010108
Russell TM, Richardson DR. Glutathione S-transferases as potential targets for modulation of nitric oxide-mediated vasodilation. Biomolecules. 2022;12(9):1292. DOI: 10.3390/biom12091292
Chai YC, Mieyal JJ. Glutathione and glutaredoxin: key players in cellular redox homeostasis and signaling. Antioxidants. 2023;12(8):1553. DOI: 10.3390/antiox12081553
Belenichev I, Abramov A, Puzyrenko A, Bukhtiyarova N, Gorchakova N, Bak P. Molecular mechanisms of myocardial damage in hypertensive rats and hypertensive rats with metabolic disorders. Res Results Pharmacol. 2022;8(4):25–33. DOI: 10.3897/rrpharmacology.8.78534
Serreau R, Amirouche A, Benyamina A, Berteina-Raboin S. Propranolol hydrochloride psychiatric effectiveness and oxidative stress: an update. Oxygen. 2024;4(2):139–149. DOI: 10.3390/oxygen4020009
Ranasinghe HN, Fernando N, Handunnetti S, Weeratunga PN, Katulanda P, Rajapakse S, et al. The impact of propranolol on nitric oxide and total antioxidant capacity in patients with resistant hypertension: evidence from the APPROPRIATE trial. BMC Res Notes. 2020;13(1):228. DOI: 10.1186/s13104-020-05067-5
Dandona P, Ghanim H, Brooks DP. Antioxidant activity of carvedilol in cardiovascular disease. J Hypertens. 2007;25(4):731–741. DOI: 10.1097/HJH.0b013e3280127948
Ayashi S, Assareh AR, Jalali MT, Olapour S, Yaghooti H. Role of antioxidant property of carvedilol in mild to moderate hypertensive patients: a prospective open-label study. Indian J Pharmacol. 2016;48(4):372–376. DOI: 10.4103/0253-7613.186206
Turco L, Reiberger T, Vitale G, La Mura V. Carvedilol as the new non-selective beta-blocker of choice in patients with cirrhosis and portal hypertension. Liver Int. 2023;43:1183–1194. DOI: 10.1111/liv.15559
Rewish NM, Elmorsy MR, Fadda AA, Badawy SA. Design, synthesis, and evaluation of novel triazole-based carbohydrazide derivatives with notable antioxidant activity. BMC Chem. 2026;20(1):48. DOI: 10.1186/s13065-026-01736-x
Li Q, Qi S, Liang J, Tian Y, He S, Liao Q, et al. Review of triazole scaffolds for treatment and diagnosis of Alzheimer’s disease. Chem Biol Interact. 2023;382:110623. DOI: 10.1016/j.cbi.2023.110623
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