Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes
DOI:
https://doi.org/10.20535/ibb.2023.7.4.284774Keywords:
sperm quality, sperm motility, zinc, boar semen, antioxidantsAbstract
Background. Zinc is essential for male reproductive function as it is necessary for spermatozoa maturation, capacitation, acrosome reaction, and fertilization. It has been established that the use of chelates compounds of metals with amino acids, nucleotides, peptides, and carbohydrates is not only more effective but also economically justified.
Objective. The aim of this study was to evaluate and compare the effect of different concentrations of Zn glutamic amino acid chelate (ZnGlu) on boar spermatozoa motility, viability parameters, and prooxidant-antioxidant homeostasis during in vitro incubation.
Methods. Freshly ejaculated boar semen, after the addition of "Ecosperm" diluent, was divided into groups: a control group and three experimental groups. Zinc glutamate was supplemented to the experimental samples at concentrations of 1.0, 2.0, and 5.0 µg/ml, respectively. Semen samples were stored at 18 °C for 4 days. Every 24 hours, sperm motility and viability and the antioxidant status were assessed by the level of diene conjugates (DC), concentration of malondialdehydere (MDA), and the activities of catalase and superoxide dismutase.
Results. It was observed that boar sperm motility decreased with the extension of storage time, while the addition of 2.0 and 5.0 µg ZnGlu significantly improved sperm total motility and the percentage of vitality spermatozoa during 48–96 hours of incubation. Our studies also demonstrate that ZnGlu possess a protective effect in alleviating oxidative stress in boar sperm in vitro. The addition of ZnGlu significantly reduced the content of MDA and DC in ejaculate samples in all experimental groups during incubation, compared to the control group. Moreover, the activity of superoxide dismutase and catalase increased after adding ZnGlu to the boar semen, especially at concentrations of 2.0 and 5.0 mg/ml (P < 0.05).
Conclusions. These data demonstrate that the supplementation of zinc glutamate enhances the antioxidant defence system of sperm and improves quality of boar semen in vitro.
References
Vickram AS, Samad HA, Latheef SK, Chakraborty S, Dhama K, Sridharan TB, Sundaram T, Gulothungan G Human prostasomes an extracellular vesicle–Biomarkers for male infertility and prostate cancer: The journey from identification to current knowledge. Int J Biol Macromol. 2020;146:946-58. DOI: 10.1016/j.ijbiomac.2019.09.218
Fallah A, Mohammad-Hasani A, Colagar AH. Zinc is an essential element for male fertility: A review of Zn roles in men's health, germination, sperm quality, and fertilization. J Reprod Infertil. 2018 Apr-Jun;19(2):69-81.
Allouche-Fitoussi D, Breitbart H. The role of Zinc in male fertility. Int J Mol Sci. 2020 Oct 21;21(20):7796. DOI: 10.3390/ijms21207796
Vickram S, Rohini K, Srinivasan S, Nancy Veenakumari D, Archana K, Anbarasu K, et al. Role of Zinc (Zn) in human reproduction: a journey from initial spermatogenesis to childbirth. Int J Mol Sci. 2021 Feb 22;22(4):2188. DOI: 10.3390/ijms22042188
Garratt M, Bathgate R, de Graaf SP, Brooks RC. Copper-zinc superoxide dismutase deficiency impairs sperm motility and in vivo fertility. Reproduction. 2013 Aug 12;146(4):297-304. DOI: 10.1530/REP-13-0229
Colagar AH, Marzony ET, Chaichi MJ. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr Res. 2009 Feb;29(2):82-8. DOI: 10.1016/j.nutres.2008.11.007
James ER, Carrell DT, Aston KI, Jenkins TG, Yeste M, Salas-Huetos A. The role of the epididymis and the contribution of epididymosomes to mammalian reproduction. Int J Mol Sci. 2020 Jul 29;21(15):5377. DOI: 10.3390/ijms21155377
Zhu Z, Kawai T, Umehara T, Hoque SAM, Zeng W, Shimada M. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria. Free Radic Biol Med. 2019 Sep;141:159-71. DOI: 10.1016/j.freeradbiomed.2019.06.018
Hara T, Takeda TA, Takagishi T, Fukue K, Kambe T, Fukada T. Physiological roles of zinc transporters: molecular and genetic importance in zinc homeostasis. J Physiol Sci. 2017 Mar;67(2):283-301. DOI: 10.1007/s12576-017-0521-4.
Peña ST Jr, Gummow B, Parker AJ, Paris DBBP. Antioxidant supplementation mitigates DNA damage in boar (Sus scrofa domesticus) spermatozoa induced by tropical summer. PLoS One. 2019 Apr 30;14(4):e0216143. DOI: 10.1371/journal.pone.0216143
Guthrie HD, Welch GR. Effects of reactive oxygen species on sperm function. Theriogenology. 2012 Nov;78(8):1700-8. DOI: 10.1016/j.theriogenology.2012.05.002
Brouwers JF, Silva PF, Gadella BM. New assays for detection and localization of endogenous lipid peroxidation products in living boar sperm after BTS dilution or after freeze-thawing. Theriogenology. 2005 Jan 15;63(2):458-69. DOI: 10.1016/j.theriogenology.2004.09.046
Feng C, Zhu Z, Bai W, Li R, Zheng Y, Tian X, et al. Proline protects boar sperm against oxidative stress through proline dehydrogenase-mediated metabolism and the amine structure of pyrrolidine. Animals (Basel). 2020 Sep 1;10(9):1549. DOI: 10.3390/ani10091549
Pintus E, Kadlec M, Jovičić M, Sedmíková M, Ros-Santaella JL. Aminoguanidine protects boar spermatozoa against the deleterious effects of oxidative stress. Pharmaceutics. 2018 Nov 1;10(4):212. DOI: 10.3390/pharmaceutics10040212
Huang YL, Lu L, Li SF, Luo XG, Liu B. Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet. J Anim Sci. 2009 Jun;87(6):2038-46. DOI: 10.2527/jas.2008-1212
Zafar MH, Fatima M. Efficiency comparison of organic and inorganic minerals in poultry nutrition: A review. PSM Veterinary. Res. 2018;3(2):53-9
Maares M, Haase H. A guide to human zinc absorption: General overview and recent advances of in vitro intestinal models. Nutrients. 2020 Mar 13;12(3):762. DOI: 10.3390/nu12030762
Min YN, Liu FX, Qi X, Ji S, Cui L, Wang ZP, et al. Effects of organic zinc on tibia quality, mineral deposit, and metallothionein expression level of aged hens. Poult Sci. 2019 Jan 1;98(1):366-72. DOI: 10.3382/ps/pey386
Byrne L, Murphy RA. Relative bioavailability of trace minerals in production animal nutrition: A review. Animals. 2022;12(15):1981. DOI: 10.3390/ani12151981
Moskovtsev SI, Librach CL. Methods of sperm vitality assessment. In: Spermatogenesis: Methods and Protocols. Humana Press, Totowa, NJ. 2013;927:13-9. DOI: 10.1007/978-1-62703-038-0_2
Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of investigation in biology, stockbreeding and veterinary, 2nd ed. Lviv: Spolom, 2012; p. 764.
Kasperczyk S, Słowińska-Łożyńska L, Kasperczyk A, Wielkoszyński T, Birkner E. The effect of occupational lead exposure on lipid peroxidation, protein carbonylation, and plasma viscosity. Toxicol Ind Health. 2015 Dec;31(12):1165-71. DOI: 10.1177/0748233713491804
Polishchuk S, Tsekhmistrenko S, Polishchuk V, Tsekhmistrenko O, Zdorovtseva L, Kotula-Balak M, et al. Status of prooxidant and antioxidant systems in the sperm and seminal plasma of breeding boars of large white breed and SS23 synthetic line. J Physiol Pharmacol. 2022 Feb;73(1). DOI: 10.26402/jpp.2022.1.07
Kerns K, Sharif M, Zigo M, Xu W, Hamilton LE, Sutovsky M, et al. Sperm cohort-specific zinc signature acquisition and capacitation-induced zinc flux regulate sperm-oviduct and sperm-zona pellucida interactions. Int J Mol Sci. 2020 Mar 19;21(6):2121. DOI: 10.3390/ijms21062121
Milostić-Srb A, Včev A, Tandara M, Marić S, Kuić-Vadlja V, Srb N, et al. Importance of zinc concentration in seminal fluid of men diagnosed with infertility. Acta Clin Croat. 2020 Mar;59(1):154-60. DOI: 10.20471/acc.2020.59.01.19
Türk S, Mändar R, Mahlapuu R, Viitak A, Punab M, Kullisaar T. Male infertility: decreased levels of selenium, zinc and antioxidants. J Trace Elem Med Biol. 2014 Apr;28(2):179-85. DOI: 10.1016/j.jtemb.2013.12.005
Marini P, Fernández Beato L, Cane F, Teijeiro JM. Effect of zinc on boar sperm liquid storage. Front Vet Sci. 2023 Feb 2;10:1107929. DOI: 10.3389/fvets.2023.1107929
Turgut G, Abban G, Turgut S, Take G. Effect of overdose zinc on mouse testis and its relation with sperm count and motility. Biol Trace Elem Res. 2003;96(1-3):271-9. DOI: 10.1385/BTER:96:1-3:271
Zhao J, Dong X, Hu X, Long Z, Wang L, Lui Q, et al. Zinc levels in seminal plasma and their correlation with male infertility: A systematic review and meta-analysis. Sci Rep. 2016 March 2;6:22386. DOI: 10.1038/srep22386
Kotdawala AP, Kumar S, Salian SR, Thankachan P, Govindraj K, Kumar P, et al. Addition of zinc to human ejaculate prior to cryopreservation prevents freeze-thaw-induced DNA damage and preserves sperm function. J Assist Reprod Genet. 2012 Dec;29(12):1447-53. DOI: 10.1007/s10815-012-9894-8
Khoi HX, Shimizu K, Yoneda Y, Minagawa I, Abe Y, Kuwabara Y, et al. Monitoring the reactive oxygen species in spermatozoa during liquid storage of boar semen and its correlation with sperm motility, free thiol content and seasonality. Andrologia. 2021 Sep 06;53(11):e14237. DOI: 10.1111/and.14237
Cui N, Hu M, Khalil RA. Biochemical and biological attributes of matrix metalloproteinases. In: Matrix Metalloproteinases and Tissue Remodeling in Health and Disease: Cardiovascular Remodeling. 2017;147:1-73. DOI: 10.1016/bs.pmbts.2017.02.005
Kerns K, Zigo M, Sutovsky P. Zinc: A necessary ion for mammalian sperm fertilization competency. Int J Mol Sci. 2018 Dec 18;19(12):4097. DOI: 10.3390/ijms19124097
Zigo M, Manaskova-Postlerova P, Jonakova V, Kerns K, Sutovsky P. Compartmentalization of the proteasome-interacting proteins during sperm capacitation. Sci Rep. 2019 Aug 29;9(1):12583. DOI: 10.1038/s41598-019-49024-0
Ferreira GM, Annandale CH, Smuts MP, Holm DE. The potential effects and interactions of oxidative stress and trace minerals on fresh and frozen semen in bulls - a review. J S Afr Vet Assoc. 2022 Nov;93(2):70-5. DOI: 10.36303/jsava.02
Venkata Krishnaiah M, Arangasamy A, Selvaraju S, Guvvala PR, Ramesh K. Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus). Theriogenology. May 2019;130:130-9. DOI: 10.1016/j.theriogenology.2019.02.026
Schlegel P, Sauvant D, Jondreville C. Bioavailability of zinc sources and their interaction with phytates in broilers and piglets. Animal. 2013 Jan;7(1):47-59. DOI: 10.1017/S1751731112001000
Chen X, He C, Zhang K, Wang J, Ding X, Zeng Q, et al. Comparison of zinc bioavailability in zinc-glycine and zinc-methionine chelates for broilers fed with a corn-soybean meal diet. Front Physiol. 2022 Nov 17;13:983954. DOI: 10.3389/fphys.2022.983954
Hemalatha K, Arangasamy A, Selvaraju S, Venkata Krishnaiah M, Rani GP, Mishra A, et al. Effect of dietary supple-mentation of organic zinc and copper on in vitro semen fertility in goat. Small Ruminant Res. 2018 April; 161:68-72. DOI: 10.1016/j.smallrumres.2018.02.003
Kumar N, Verma RP, Singh LP, Varshney VP, Dass RS. Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus x Bos taurus) bulls. Reprod Nutr Dev. 2006 Nov-Dec;46(6):663-75. DOI: 10.1051/rnd:2006041
Mousavi Esfiokhi SH, Norouzian MA and Najafi A. Effect of different sources of dietary zinc on sperm quality and oxidative parameters. Front Vet Sci. 2023 June;10. DOI: 10.3389/fvets.2023.1134244
Vollmer T, Ljungberg B, Jankowski V, Jankowski J, Glorieux G, Stegmayr BG. An in-vitro assay using human spermatozoa to detect toxicity of biologically active substances. Sci Rep. 2019 Oct 10;9(1):14525. DOI: 10.1038/s41598-019-50929-z
Moretti E, Signorini C, Corsaro R, Giamalidi M, Collodel G. Human sperm as an in vitro model to assess the efficacy of antioxidant supplements during sperm handling: A narrative review. Antioxidants (Basel). 2023 May 15;12(5):1098. DOI: 10.3390/antiox12051098
Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. Etiologies of sperm oxidative stress. International Journal of Reproductive BioMedicine. 2016 Apr;14(4):231-40. DOI: 10.29252/ijrm.14.4.231
Zhang R, Liang C, Guo X, Bao P, Pei J, Wu F, et al. Quantitative phosphoproteomics analyses reveal the regulatory mechanisms related to frozen-thawed sperm capacitation and acrosome reaction in yak (Bos grunniens). Front. Physiol. 2022 October 06;13:1013082. DOI: 10.3389/fphys.2022.1013082
Mannucci A, Argento FR, Fini E, Coccia ME, Taddei N, Becatti M, et al. The impact of oxidative stress in male infertility. Front Mol Biosci. 2022 Jan 5;8:799294. DOI: 10.3389/fmolb.2021.799294
Коrnyat S, Sharan М, Оstapiv D, Коrbeckij А, Jaremchuk І, Аndrushko О. Quality of deconserved bull sperm for the action of nanosuccinates Zn, Cu and Mn in the diluents. Anim Biol. 2021;23(1):23-9. DOI: 10.15407/animbiol23.01.023
Hu Q. Effects of zinc chloride on boar sperm quality during liquid storage at 17C. Vet Med Sci. 2023 May;9(3):1217-25. DOI: 10.1002/vms3.1094
Neamah HJ, AL-Omairi SJF, Al-Sarray MAR. The effect of zinc oxide on the glutathione peroxidase and superoxide dismutase enzymes activities of ram sperm at cooling. Iran J Ichthyol. 2022;1:462-6.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Оксана Сливчук, Оксана Штапенко, Ірина Яремчук, Сергій Корнят, Євген Дзень
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.
IBB articles are published under Creative Commons licence:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
