Biosynthetic Potential of Actinomycetes from Helianthemum stevenii Rupr. Ex Juz. & Pozd. Rhizosphere
DOI:
https://doi.org/10.20535/ibb.2019.3.2.170129Keywords:
Actinomycetes, Antagonistic properties, Phytostimulation, Crimean peninsula, Biosynthetic potentialAbstract
Background. Today the actual problems of humanity are the high frequency of the emergence of new multi-resistant pathogenic microorganisms, lack of food and pollution, especially agro-ecosystems. Screening of new natural products and introducing them in medicine, veterinary medicine, agriculture, etc. is one of the approaches to solving these problems. Objects of particular interest are actinomycetes – record holders in the production of biologically active compounds.
Objective. The purpose of the paper is evaluation of the biosynthetic properties of actinomycetes from the rhizosphere of the endemic Helianthemum stevenii Rupr. Ex Juz & Pozd. from Crimean peninsula.
Methods. Samples of the H. stevenii Rupr. Ex Juz & Pozd. rhizosphere were taken on the southern slopes of the Nikita Range (Crimean Peninsula). Actinomycetes were isolated by direct sowing of the washed rhizosphere, aqua solution of phenol treatment of the roots, or by heating them at 100 °C within 60 minutes and sowing on the nutrient medium. Antibacterial and anti-candidiasis activities were studied by culturing the actinomycetes strains by a prick on the oat medium and pouring the 0.7% agar with a certain test culture. Antifungal properties were studied by putting the agar block with a 5-day fungal culture on cups with 3-day cultures of actinomycetes and subsequent incubation for 4 days. An activity index (AI) was determined by the ratio of the diameter zone of the inhibit growth test-cultures to the diameter of the actinomycetes colonies. Phytostimulants and enzymatic properties were studied by commonly accepted methods.
Results. 107 actinomycetes strains from H. stevenii Rupr. Ex Juz. & Pozd. rhizosphere were isolated. 23.2% of isolates inhibited growth of one or more typical microbial test cultures which are capable of causing a disease in humans. 8.4% of the actinomycetes strains were antagonists of S. аureus, the less actinomycetes strains inhibited gram-negative test cultures. AI of these strains was no more than 3. 80.1% of isolates were antagonists of at least one strain of the phytopathogenic bacteria. AI of these strains was from 1.2 to 13. Antifungal activity was in the 15.9% of isolates and their AI was no more than 3. 17.8 of actinomycetes strains were capable of producing indole-3-acetic acid, 10.3% – siderophores and 14.2% – phosphate solubilizers. A significant proportion of actinomycetes strains was capable of producing hydrolytic enzymes: amylases, lipases, pectinases and proteases. 29.9% of isolates can discolour Azure B and 8.4% are potentially laccase producers.
Conclusions. The ability of actinomycetes strains from H. stevenii Rupr. Ex Juz. & Pozd. rhizosphere to produce antimicrobial compounds, enzymes and plants growth promoting (PGP) compounds was evaluated. Actinomycetes strains with wide range and specific inhibit certain pathogenic strain were selected as well as strains that specifically inhibit the growth of a particular strain of pathogenic microorganisms. A number of phytopathogenic bacteria antagonists tend to phytostimulation. The producers of hydrolases and oxidoreductase were isolated.References
Higgins PG, Dammhayn C, Hackel M, Seifert H. Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother. 2010;65(2):233-38. DOI: 10.1093/jac/dkp428
Bilyk O, Luzhetskyy A. Metabolic engineering of natural product biosynthesis in actinobacteria. Curr Opin Biotechnol. 2016;42:98-107. DOI: 10.1016/j.copbio.2016.03.008
Hoffman T, Krug D, Bozkurt N, Duddela S, Jansen R, Garcia R, et al. Correlating chemical diversity with taxonomic distance for discovery of natural products in myxobacteria. Nat Commun. 2018;9(1):803. DOI: 10.1038/s41467-018-03184-1
Iqbal H, Low-Beinart L, Obiajulu J, Brady S. Natural product discovery through improved functional metagenomics in Streptomyces. J Am Chem Soc. 2016;138(30):9341-44. DOI: 10.1021/jacs.6b02921
Polanski J, Bogocz J, Tkocz A. Top 100 bestselling drugs represent an arena struggling for new FDA approvals: drug age as an efficiency indicator. Drug Discov Today. 2015;20(11):1300-4. DOI: 10.1016/j.drudis.2015.06.015
Behie SW, Bonet B, Zacharia VM, McClung DJ, Traxler MF. Molecules to ecosystems: Actinomycete natural products in situ. Front Microbiol. 2016;7:2149. DOI: 10.3389/fmicb.2016.02149
Kealey C, Creaven CA, Murphy CD, Brady CB. New approaches to antibiotic discovery. Biotechnol Lett. 2017;39(6):805-17. DOI: 10.1007/s10529-017-2311-8
Manivasagan P, Venkatesan J, Sivakumar K, Kim S-K. Pharmaceutically active secondary metabolites of marine actinobacteria. Microbiol Res. 2014;169:262-78. DOI: 10.1016/j.micres.2013.07.014
Matsumoto A, Takahashi Y. Endophytic actinomycetes: promising source of novel bioactive compounds. J Antibiot (Tokyo). 2017;70(5):514-9. DOI: 10.1038/ja.2017.20
Bhatti AA, Haq S, Bhat RA. Actinomycetes benefaction role in soil and plant health. Microb Pathog. 2017;111:458-67. DOI: 10.1016/j.micpath.2017.09.036
Lewis K. New approaches to antimicrobial discovery. Biochem Pharmacol. 2017;15(134):87-98. DOI: 10.1016/j.bcp.2016.11.002
Didukh YaP, Ed. Biotopes of the Crimean Mountains. Кyiv: NVP Interservis; 2016. 292 p.
Gromyko O. Antifungal and antibacterial properties of actinomyces strains isolated from the rhizosphere of Juniperus excelsa Bieb. Visnyk of Lviv Univ Biol Ser. 2010;53:156-60.
Gromyko O. Antagonistic properties of actinomycetes from the ryzosphere of Olea europaea L. Visnyk of Lviv Univ Biol Ser. 2012;59:209-15.
Raju R, Gromyko O, Butsiak A, Fedorenko V, Luzhetskyy A, Müller R. Oleamycins A and B: new antibacterial cyclic hexadepsipeptides isolated from a terrestrial Streptomyces sp. J Antibiotics. 2014;67:339-43. DOI: 10.1038/ja.2014.1
Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Müller R. Oleaceran: A novel Spiro[isobenzofuran-1,2'-naptho[1,8-bc]furan] isolated from a terrestrial Streptomyces sp. Organic Lett. 2013;15(14):3487-9. DOI: 10.1021/ol401490u
Raju R, Gromyko O, Fedorenko V, Luzhetskyy A, Plaza A, Müller R. Juniperolide A: A new polyketide isolated from a terrestrial actinomycete, Streptomyces sp. Organic Lett. 2012;14(23):5860-3. DOI: 10.1021/ol302766z
Raju R, Gromyko O, Fedorenko V, Herrmann J, Luzhetskyy A, Müller R. Rubimycinone A, a new anthraquinone from a terrestrial Streptomyces sp. Tetrahedron let. 2013;54(8):900-2. DOI: 10.1016/j.tetlet.2012.11.130
Gauze GF, Preobrazhenskaya TP, Sveshnikova MA, Terehova LP, Maksimova TS. Key to actinomycetes. Genus Streptomyces. Moscow: Nauka; 1983. 245 p.
Zenova GM. Soil actinomycetes. Moscow; 1992. 79 p.
Zhang J. Improvement of an isolation medium for actinomycetes. Modern App Sci. 2011;5(2):124-7. DOI: 10.5539/mas.v5n2p124
Kieser T, Bibb M, Buttner M, Chater K, Hopwood D. Practical Streptromyces genetics. Norwich: John Innes Foundation; 2000. 634 p.
Sarwar M, Kremer RJ. Determination of bacterially derived auxins by a microplate method. Lett Appl Microbiol. 1995;20:282-5. DOI: 10.1111/j.1472-765X.1995.tb00446.x
Verma V, Joshi K, Mazumdar B. Study of siderophore formation in nodule-forming bacterial species. Res J Chem Sci. 2012;2(11):26-9. DOI: 10.1007/s12088-016-0591-7
Muromtsev GS. Some methods for studying the dissolution of calcium phosphates by microorganisms. Microbiologiya. 1957;26:172-8.
Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH, Sackin MJ. Numerical classification of Streptomyces and related genera. J Gen Microbiol. 1983;129(6):1743-813. DOI: 10.1099/00221287-129-6-1743
Fernandes TAR, da Silveira WB, Passos FML, Zucchi TD. Characterization of a thermotolerant laccase produced by Streptomyces sp. SB086 Ann Microbiol. 2014;64:1363-9. DOI: 10.1007/s13213-013-0781-z
Li H, Zhang R, Tang L, Zhang J, Mao Z. Evaluation of Bacillus sp. MZS10 for decolorizing Azure B dye and its decolorization mechanism. J Environ Sci (China). 2014;26(5):1125-34. DOI: 10.1016/S1001-0742(13)60540-9
Donate-Correa J, León-Barrios M, Pérez-Galdona R. Screening for plant growth-promoting rhizobacteria in Chamaecytisus proliferus (tagasaste), a forage tree-shrub legume endemic to the Canary Islands. Plant Soil. 2005;266:261-71. DOI: 10.1007/s11104-005-0754-5
Qin S, Li J, Chen HH, Zhao GZ, Zhu WY, Jiang CL, et al. Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. App Environ Microbiol. 2009;75(19):6176-86. DOI: 10.1128/AEM.01034-09
Sathya A, Vijayabharathi R, Gopalakrishnan S. Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes. 3 Biotech. 2017;7(2):102. DOI: 10.1007/s13205-017-0736-3
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2019 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.
IBB articles are published under Creative Commons licence:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
