Activated Sludge Biomass as Magnetic Biosorbent
DOI:
https://doi.org/10.20535/ibb.2018.2.4.147292Keywords:
Activated sludge, Magnetic nanoparticles, Biomineralization, Μagnetospirillum gryphiswaldense MSR-1, Mam-proteins, Biosorption, Magnetic sorbent, High gradient magnetic separationAbstract
Background. Heavy metal pollution is a typical issue for a number of industries, and their removal is crucial for the environmental ecology. Biomass can be used as cheap and eco-friendly sorbent, for example excess activated sludge, because its utilization is a major expense in water treatment. Sorbents with magnetic properties can be extracted using fast and efficient magnetic separation method. Mechanism of biomineralization biogenic magnetic nanoparticles (BMN) is proved to be general for all three domains, so it is important to study what amount of activated sludge microorganisms are potential BMN producents.
Objectives. The objective of this study is to determine potential BMN producents among microorganisms of activated sludge using methods of comparative genomics, to obtain magnetic biosorbent and investigate its efficiency in Fe2+ removal from FeSO4 solution (500 mg/L).
Methods. For estimating homologies between biomineralization proteins of Μagnetospirillum gryphiswaldense MSR-1 and microorganisms of activated sludge were used methods of pairwise and multiple genomic sequence alignment using free access tool BLAST (National Centre for Biotechnological Information, USA). For obtaining magnetic sorbent from activated sludge biomass high gradient magnetic separation was used.
Results. Bioinformatic analysis showed, that among studied microorganisms of activated sludge, whose genomes are sequenced per 25% and more, all appeared to be potential BMN producers (Stentor coeruleus, Bodo saltans, Sphaerotilus natans, Beggiatoa alba B18LD, Oscillatoria acuminata PCC 6304, Oscillatoria sp. PCC 10802, Oscillatoria nigro-viridis PCC 7112, Rivularia sp. PCC 7116, Anabaena sp. CRKS33, Anabaena sp. WA113, Anabaena sp. AL93, Anabaena sp. PCC7108, Anabaena MDT 14b, Thiothrix nivea DSM 5205, Duganella zoogloeoides ATCC 25935), 12 of them – potential producents of intracellular crystalline BMN. It was shown, that Fe2+ removal efficiency isequal for non-separated activated sludge and both magnetic and non-magnetic fractions.
Conclusions. Results show that excess sludge biomass can be used to obtain magnetic sorbent, which can be made without the use of extra magnetization with artificial magnetic nanoparticles. This allows extracting sorbent using methods of high gradient magnetic separation. Sorption results of Fe2+ for magnetically labelled biosorbent on the base of activated sludge microorganisms experimentally proves equal efficiency of magnetically labelled, non-labelled fractions and biomass which was not exposed to HGMS and is higher than 97%.References
Aslan S, Yildiz S, Ozturk M. Biosorption of Cu2+ and Ni2+ ions from aqueous solutions using waste dried activated sludge biomass. Pol J Chem Tech. 2018;20(3):20-28. DOI: 10.2478/pjct-2018-0034
Yayintas OT, Yilmaz S, Turkoglu M, Dilgin Y. Determination of heavy metal pollution with environmental physicochemical parameters in waste water of Kocabas Stream (Biga, Canakkale, Turkey) by ICP-AES. Environ Monit Assess. 2007 Apr;127(1-3):389-397. DOI: 10.1007/s10661-006-9288-4
Pagnanelli F, Mainelli S, Bornoroni L, Dionisi D, Toro L. Mechanisms of heavy-metal removal by activated sludge. Chmosphere. 2009 May;75(8):1028-1034. DOI: 10.1016/j.chemosphere.2009.01.043
Gorobets SV, Kasatkina TP, Gorobets ОYu, Ukrainetz AI, Goyko IYu. Intensification of copper and chrome ions sorption by yeast of Saccharomyces cerevisiae in the magnetic field. Kharchova Promyslovist. 2004;3:107-9.
Jianlong W, Yi Q, Horan N, Stentiford E. Bioadsorption of pentachlorophenol (PCP) from aqueous solution by activated sludge biomass. Bioresour Technol. 2000 May;75(2):157-161. DOI: 10.1016/S0960-8524(00)00041-9
Liu D, Tao Y, Li K, Yu J. Influence of the presence of three typical surfactants on the adsorption of nickel (II) to aerobic activated sludge. Bioresour Technol. 2012 Dec;126:56-63. DOI: 10.1016/j.biortech.2012.09.025
Pamukoglu MY, Kargi F. Removal of copper(II) ions from aqueous medium by biosorption onto powdered waste sludge. Process Biochem. 2006 May;41(5):1047-1054. DOI: 10.1016/j.procbio.2005.11.010
Wei D, Zhang K, Wang S, Sun B, Wu N, Xu W, et al. Characterization of dissolved organic matter released from activated sludge and aerobic granular sludge biosorption processes for heavy metal treatment via a fluorescence approach. Int Biodeterior Biodegrad. 2017 March;124:326-333. DOI: 10.1016/j.ibiod.2017.03.018
Yuncu B, Sanin FD, Yetis U. An investigation of heavy metal biosorption in relation to C/N ratio of activated sludge. J Hazard Mater. 2006 Sep 21;137(2): 990-997. DOI: 10.1016/j.jhazmat.2006.03.020
Zhou Y, Zhang Z, Zhang J, Xia S. New insight into adsorption characteristics and mechanisms of the biosorbent from waste activated sludge for heavy metals. J Environ Sci. 2016 Jul;45:248-256. DOI: 10.1016/j.jes.2016.03.007
Zhang Q, Hu J, Lee DJ, Chang Y, Lee YJ. Sludge treatment: Current research trends. Bioresour Technol. 2017 Nov;243:1159-1172. DOI: 10.1016/j.biortech.2017.07.070
Perez-Elvira SI, Nieto Diez P, Fdz-Polanco F. Sludge minimisation technologies. Rev Environ Sci Biotechnol. 2006 Nov;5:375-398. DOI: 10.1007/s11157-005-5728-9
Nuhoglu Y, Oguz E. Removal of copper(II) from aqueous solutions by biosorption on the cone biomass of Thuja orientalis. Prosess Biochem. 2003 June 30;38:1627-1631. DOI: 10.1016/S0032-9592(03)00055-4
Hammaini A, González F, Ballester A, Blázquez ML, Muñoz JA. Biosorption of heavy metals by activated sludge and their desorption characteristics. J Environ Manage. 2007 Sep;84(4):419-426. DOI: 10.1016/j.jenvman.2006.06.015
Kargi F, Cikla S. Biosorption of zinc(II) ions onto powdered waste sludge (PWS): Kinetics and isotherms. Enzyme Microb. Technol. 2006 March;38(5):705-710. DOI: 10.1016/j.enzmictec.2005.11.005
Laurent J, Casellas M, Pons MN, Dagot C. Cadmium biosorption by ozonized activated sludge: The role of bacterial flocs surface properties and mixed liquor composition. J Hazard Mater. 2010 Nov 15;183(1-3):256-263. DOI: 10.1016/j.jhazmat.2010.07.019
Ong SA, Toorisaka E, Hirata M, Hano T. Comparative study on kinetic adsorption of Cu(II), Cd(II) and Ni(II) ions from aqueous solutions using activated sludge and dried sludge. Appl Water Sci. 2013 March;3:321-325. DOI: 10.1007/s13201-013-0084-3
Rao PR, Bhargavi C. Studies on biosorption of heavy metals using pretreated biomass of fungal species. Int J Chem Chem Eng. 2013;3(3):171-180.
Yang C, Wang J, Lei M, Xie G, Zeng G, Luo S. Biosorption of zinc (II) from aqueous solution by dried activated sludge. J Environ Sci. 2010;22(5):675-680. DOI: 10.1016/S1001-0742(09)60162-5
Zhmur NA. Technological and biochemical processes of wastewater treatment at buildings with aeration tanks. Moscow: Aquaros; 2003. 512 p.
Sakaguchi T, Burgess JG, Matsunaga T. Magnetite formation by a sulphate-reducing bacterium. Nature. 1993;365:47-49. DOI: 10.1038/365047a0
Blakemore R. Magnetotactic bacteria. Science. 1975 Oct 24;190(4212):377-379. DOI: 10.1126/science.170679
Frankel RB, Blakemore RP, Wolfe RS. Magnetite in freshwater magnetotactic bacteria. Science. 1979 Mar;203(4387):1355-1356. DOI: 10.1126/science.203.4387.1355
Bazylinski DA, Frankel RB. Magnetosome formation in prokaryotes. Nat Rev Microbiol. 2004 Mar;2(3):217-230. DOI: 10.1038/nrmicro842
Vainshtein M, Suzina N, Kudryashova E, Ariskina E. New magnet-sensitive structures in bacterial and archaeal cells. Biol Cell. 2002 Feb;94(1):29-35. DOI: 10.1016/S0248-4900(02)01179-6
Gorobets Ο, Gorobets S, Sorokina L. Biomineralization and synthesis of biogenic magnetic nanoparticles and magnetosensitive inclusions in microorganisms and fungi. Funct Mater. 2014;21(4):427-436. DOI: 10.15407/fm21.04.427
Gorobets SV, Mikhailenko NA. High-gradient ferromagnetic matrices for purification of wastewaters by the method of magnitoelectrolysis. J Water Chem Technol. 2014;36(4):153-159. DOI: 10.3103/S1063455X14040018
Gorobets SV, Mykhailenko NA, Makarchuk OV, Dontsova TA, Astrelin IM. Purification of aqeous media by magnetically operated saponite sorbents. Eastern-European Journal of Enterprise Technologies. 2015;4/10(67):13-20. DOI: 10.15587/1729-4061.2015.46573
Gorobets OYu, Gorobets SV, Gorobets YuI. Biogenic magnetic nanoparticles: biomineralization in prokaryotes and eukaryotes. In: Dekker Encyclopedia of Nanoscience and Nanotechnology. 3rd ed. New York: CRC Press; 2014. p. 300-8.
Gorobets OYu, Gorobets SV, Gorobets YuI. Biomineralization of intracellular biogenic magnetic nanoparticles and their expected functions. Naukovi Visti NTUU KPI. 2013;3:28-33.
Gorobets O, Gorobets S, Koralewski M. Physiological origin of biogenic magnetic nanoparticles in health and disease: from bacteria to humans. Int J Nanomed. 2017 Jun 12;12:4371-4395. DOI: 10.2147/IJN.S130565
National Center for Biotechnology Information [Internet]. Bethesda, Maryland, U.S.: NCBI; 1988. Available from: https://www.ncbi.nlm.nih.gov
Ullrich S, Kube M, Schübbe S, Reinhardt R, Schüler D. A Hypervariable 130-kilobase genomic region of Magnetospirillum gryphiswaldense comprises a magnetosome island which undergoes frequent rearrangements during stationary growth. J Bacteriol. 2005 Nov; 187(21):7176-7184. DOI: 10.1128/JB.187.21.7176-7184.2005
Schübbe S, Würdemann C, Peplies J, Heyen U, Wawer C, Glockner F, et al. Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense. Appl Environ Microbiol. 2006 Sep;72(9):5757-5765. DOI: 10.1128/AEM.00201-06
National Center for Biotechnology Information. BLAST Assembled Genomes [Internet]. Bethesda, Maryland, U.S.: NCBI; 1988. Available from: http://blast.ncbi.nlm.nih.gov
Schüler D, Baeuerlein E. Iron-limited growth and kinetics of iron uptake in Magnetospirillum gryphiswaldense. Arch Microbiol. 1996 Nov;166(5):301-307. DOI: 10.1007/s002030050387
Li W, Pio F, Pawłowski K, Godzik A. Saturated BLAST: an automated multiple intermediate sequence search used to detect distant homology. Bioinformatics. 2000 Dec;16(12):1105-1110. DOI: 10.1093/bioinformatics/16.12.1105
Ukrainian Scientific Centre for the Protection of Waters. Guidance document 11.1.4.023-95. Method of determination of biochemical oxygen consumption after n days (BSC) in natural and sewage waters. Kyiv: Ukrainian Scientific Centre for the Protection of Waters; 1995.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 The Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.
IBB articles are published under Creative Commons licence:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
