Formation and Properties Polymer Nanolayers to Enhance Cell Growth in vitro
DOI:
https://doi.org/10.20535/ibb.2018.2.2.132515Keywords:
Cell culture, Proliferative activity, Surface, NanolayersAbstract
Background. Cultivation of cell cultures on synthetic coating makes it possible to obtain a complex spatially organized cellular system that enhances cell attachment and determines all further processes of differentiation, proliferation, and formation of extracellular matrix. It is necessary to examine properties of coatings, particularly, such as biocompatible polymers with cells, as they can be applied for various biological and medical applications.
Objective. We investigated the effects of glass surfaces modified with dextran, APTES, albumin and they compositions on the proliferation and metabolic activity of B16F10 cells.
Methods. Cellular line B16F10 were cultured in DMEM medium supplemented with 10 % fetal calf serum, 1 % penicillin-streptomycin in 5 % CO2 at 37 °C for 72 h. Cells were seeded at the glass plates, which modified nanolayers in various combinations: control group – glass, glass/APTES, glass/APTES/dextran, glass/APTES/albumin, glass/albumin, glass/APTES/dextran/albumin. The influence of the surface properties on the proliferation of B16F10culture and its viability was analyzed after every 24 h of incubation. The cultural medium was collected after 24, 48, and 72 h of cultivation for investigation lactate dehydrogenase activity.
Results. The high viability and proliferation growth of cells on APTES, albumin, and APTES/dextran/albumin coating were higher if compared with growth of cells on a glass surface. Improved the proliferation of the B16F10 cells was observed onto albumin (P < 0.001) and APTES/dextran/albumin (P < 0.001) nanolayers on 48 and 72 h, in contrast to to control and other experimental groups. Whereas, the difference between the number of cells grown on glass and APTES coating increases only on 72 h of cultivation.
Conclusions. Obtained results have shown that the glass surface modified albumin and APTES/dextran/albumin resulted in improving the viability and cell proliferation of B16F10cell line and can be used as a 3D system for cultivation of cells of different types.References
Edmondson R, Broglie JJ, Adcock AF, Yang L. Three-dimensional cell culture systems and their applications in drug discovery and cell-based biosensors. Assay Drug Dev Technol. 2014;12(4):207-18. DOI: 10.1089/adt.2014.573
Madich A, Sheremeta V, Hevkan I, Shtapenko O, Fedorova S, Slyvchuk Yu. Cell culture and its possible use in embryonic biotechnology. Kyiv: ArtEkom, 2012. 144 p.
Ishizaki T, Saito N, Takai O. Correlation of cell adhesive behaviors on superhydrophobic, superhydrophilic, and micropatterned superhydrophobic/superhydrophilic surfaces totheir surface chemistry. Langmuir. 2010 Feb;26(11):8147-54. DOI: 10.1021/la904447c
Miksa D, Irish ER, Chen D, Composto RJ, Eckmann DM. Dextran functionalized surfaces via reductive amination: morphology, wetting, and adhesion. Biomacromolecules. 2005 Dec 27;7(2):557-64. DOI: 10.1021/bm050601o
Rabe M, Verdes D, Seeger S. Surface-induced spreading phenomenon of protein clusters. Soft Matter. 2009 Aug;5:1039-47. DOI: 10.1039/B814053G
Noorisafa F, Razmjou A, Emams N, Low Z-X, Korayem H, Kajani AA. Surface modification of polyurethane via creating a biocompatible superhydrophilic nanostructured layer: role of surface chemistry and structure. J Experimental Nanosci. 2016;11(14):1087-109. DOI: 10.1080/17458080.2016.1188223
Brynda E, Houska M, Jirouskova M. Albumin and heparin multilayer coatings for blood-contacting medical devices. J Biomed Mater Res. 2000 May;51(2):249-57. DOI: 10.1002/(SICI)1097-4636(200008)51:2<249::AID-JBM14>3.0.CO;2-X
Luzinov I, Minko S, Tsukruk VV. Responsive brush layers: from tailored gradients to reversibly assembled nanoparticles. Soft Matter. 2008 Feb,4:714. DOI: 10.1039/B718999K
Bittrich E, Burkert S, Müller M, Eichhorn KJ, Stamm M, Uhlman P. Temperature-sensitive swelling of poly(N-isopropylacrylamide) brushes with low molecular weight and grafting density. Langmuir. 2012 Feb;28(7):3439-48. DOI: 10.1021/la204230a
Kaur gurbinder clinical applications of biomaterials: state-of-the-art progress, trends, and novel approaches [Internet]. Springer; 2017 [cited 22 May 2018]. 467 p. Available from: https://www.springer.com/gb/book/9783319560588
Wendy K, Scholz W. Cell adhesion and growth on coated or modified glass or plastic surfaces [Internet]. Tools.thermofisher.com. 2018 [cited 22 May 2018]. Available from: https://tools.thermofisher.com/content/sfs/brochures/D00253.pdf
Maurer E, Hussain S, Mukhopadhyay SM. Cell growth in a porous microcellular structure: Influence of surface modification and nanostructures. Nanosci Nanotechnol Lett 2011;3(1):110-3. DOI: 10.1166/nnl.2011.1128
Alves LB, de Souza SLS, Taba JM, Novaes ABJr, Oliveira PT, Palioto DB. Bioactive glass particles in two-dimensional and three-dimensional osteogenic cell cultures. Braz Dent J. 2017 May/June;28(3). DOI: 10.1590/0103-6440201600953
Legrand C, Bour JM, Jacob C, Capiaumont J, Martial A, Marc A, et al. Lactate dehydrogenase (LDH) activity of the cultured eukaryotic cells as marker of the number of dead cells in the medium. J Biotechnol. 1992;25(3):231-43. DOI: 10.1016/0168-1656(92)90158-6
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2018 The Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.
IBB articles are published under Creative Commons licence:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
