Specific Activity of Streptomyces albus Preparations for Bioreguilation of Cucumber Plant

Authors

DOI:

https://doi.org/10.20535/ibb.2018.2.2.129116

Keywords:

S. albus UN44, Biopreparation, Cucumber plants of the variety Konkurent, Biological activity, Growth stimulation, Metabolic activity, Stress resistance

Abstract

Background. Scientific bases for the development of microbial-derived polycomponent preparations for plant growing, which show a wide specificity of biological action.

Objective. The aim of the paper is an establishment of the biological activity of S. albus UN44 experimental preparations for cucumber plant bioregulation processes for further substantiation of optimization directions of ready-made forms and application methods.

 Methods. The experimental preparations were produced by cultivating the S. albus UN44 producer, in subsequently separating biomass and (in one variant) heat sterilization of supernatant. The cucumber seeds of the variety Konkurent were treated with solutions of the preparations and the growth stimulation, metabolic activity of plants, and the state of stress were determined.

Results. It is determined that Biopreparat-1 stimulates the growth of the root and stem of the cucumber after seed treatment with a solution at a concentration of 15–22 units/ml (or 1–1.5 %). It is shown, based on the data obtained, that the biological activity of culture preparations can be due to the presence of hormones of the auxin class that were not previously identified among its metabolites. For the first time, the ability of S. albus UN44 biopreparations to influence the proline content in cucumber plants, and therefore their stress resistance, is shown.

Conclusions. The results of the research are the basis for studying new biologically active products of S. albus UN44 and optimization of the ready-made forms of preparations, namely sterile liquid form and dry complex preparation, as well as preparations of certain metabolites with target highly specific activity. The ability of experimental preparations to influence the plant stress resistance requires additional study and makes it possible to create means for correcting the effects on plants of various negative factors.

References

Kyrychenko OV. Market analysis and microbial biopreparations creation for crop production in Ukraine. Biotechnologia Acta. 2015;8(4):40-52.DOI: 10.15407/biotech8.04.040

Tanaka Y, Omura S. Agroactive compounds of microbial origin. Annu Rev Microbiol. 1993;47:57-87. DOI: 10.1146/annurev.mi.47.100193.000421

Gao F, Dai C, Liu X. Mechanisms of fungal endophytes in plant protection against pathogens. Afr J Microbiol Res. 2010 Jul;4(13):1346-51.

Glick RB. Plant growth-promoting bacteria: Mechanisms and applications. Scientifica (Cairo) [Internet]. 2012 Sep [cited 2012 Sep 19];15:1-15. Available from: https://www.hindawi.com/journals/scientifica/2012/963401/cta DOI: 10.6064/2012/963401

De Jesus Sousa JA, Olivares FL. Plant growth promotion by Streptomycetes: ecophysiology, mechanisms and applications. Chem Biol Technol Agric [Internet]. 2016 Aug [cited 2016 Aug 3];3(1):1-12. Available from: https://chembioagro.springeropen.com/articles/10.1186/s40538-016-0073-5 DOI: 10.1186/s40538-016-0073-5

Biliavska LO. Actinobacteria of the genus Streptomyces and its metabolites in bioregulation of plants [Doctoral thesis]. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine; 2018. 47 p.

Zhernosekova IV, Tymchuk OA, Tkachenko VP, Vinnikov AI. Effect of metabolic products of Streptomyces recifensis var. lyticus on the vegetables sprouts growth. Mikrobiologiya i Biotehnologiya. 2014 Mar;1(25):79-90. DOI: 10.18524/2307-4663.2014.1(25).48252

Sokolova IE, Kylochek TP, Vinnikov AI. A biosynthesis activity of Streptomyces recifensis var. lyticus. Mikrobiolohichnyi Zhurnal. 2004 Nov-Dec;66(6):10-7.

Todosiichuk TT, Sichel L. A novel producer of the antibiotic substances Streptomyces albus UN 44. 14th International Conference on the Chemistry of Antibiotics and Other Bioactive Compounds; 2015; Galveston. p. 22.

Levchyk NJ, Liubinska AV, Todosiichuk TT, Rakhmetov JB, Diakova MA. The development of biological product for plant growing on the basis of Streptomyces albus. Eureka: Life Sciences. 2016;5:32-9. DOI: 10.21303/2504-5695.2016.00235

Todosiichuk TT, Sichel L, inventors. The strain of Streptomyces albus – producer of lytic enzymes complex. Ukrainian patent 09478. 2015.

Voitcehivska OV, Kapustjan AV, Kosyk OI, Musijenko MM, Olhovych OP, Panjuta OO, et al. Physiology of Plants. Lutsk: Teren; 2010. 420 p.

Wellburn AR. The spectral determination of chlorophylls a and b as well, as the total carotenoids using various solvents with spectrophotometers of different resolution. J Plant Physiol. 1994;144(3):307-13. DOI: 10.1016/S0176-1617(11)81192-2

Shihalyeyeva GN, Budnyak AK, Shihalyeyev II, Ivaschenko OL. A modified method for determination of proline in plants. The Journal of V.N. Karazin Kharkiv National University. Series: Biology. 2014 Apr;1112(21):168-72.

Kolupaev YE, Vayner AA, Yastreb TO. Proline: Physiological functions and regulation of its content in plants under stress conditions. The Bulletin of Kharkiv National Agrarian University. Series Biology. 2014 May;2(32):6-22.

Farghali KA, El-Aidarous AA. Thermostability of chlorophylls in some native species of xerophytes. IOSR-JAVS. 2014 Jan;6(6):52-65. DOI: 10.9790/2380-0665265

Kaur G, Asthir B. Proline: A key player in plant abiotic stress tolerance. Biologia Plantarum. 2015 Sep;59(4):609-19. DOI: 10.1007/s10535-015-0549-3.

Chornogor NP, Zhernosekova IV, Timchuk OA, Kilochok TP, Vinnikov AI. Phytoregulation and adaptation properties of biopreparations in conditions of technogenic pollution of the environment. Visnyk of Dnipropetrovsk University. Biology. Ecology. 2005 Oct;13(2):209-13 DOI: 10.15421/010594

Published

2018-06-25

How to Cite

1.
Levchyk N, Liubinska A, Herasymenko Y, Todosiichuk T. Specific Activity of Streptomyces albus Preparations for Bioreguilation of Cucumber Plant. Innov Biosyst Bioeng [Internet]. 2018Jun.25 [cited 2024Dec.10];2(2):98-104. Available from: https://ibb.kpi.ua/article/view/129116

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