Antimicrobial Action of Compositions Based on Thiosulfonates and Biosurfactants on Phytopathogens

Authors

DOI:

https://doi.org/10.20535/ibb.2017.1.1.112895

Keywords:

Rhamnolipid biocomplex, Thiosulfonates, Microorganism-phytopathogens, Minimum inhibitory concentration, Minimum bactericidіс concentration, Permeability of cell membrane

Abstract

Background. The development of environmentally safe preparations with antimicrobial activity on microorganism-phytopathogens.

Objective. Determination of antimicrobial activity of compositions alilthiosulfonate (ATS) and metylthiosulfonate (MTS) with surface-active rhamnolipid biocomplex (RBC) on phytopathogens Agrobacterium tumefaciens and Сlavibacter michiganensis.

Methods. Investigation in vitro of antimicrobial activity of compositions based on thiosulfonates and rhamnolipid biocomplex on microorganism-phytopathogens A. tumefaciens and C. michiganensis in accordance with the minimum inhibitory concentration and minimum bactericidic concentrations of developed compositions. Studies of biosurfactant effect on permeability of cell membraines.

Results. It is shown that the composition of methyl- and alilthiosulfanilates with RBC are more activе and have lower values of minimum inhibitory and bactericidic concentrations than ATS and MTS alone – on average by 50 %.

Conclusions. The results showed the prospects of rhamnolipid-biosurfactants use for creation of highly effective compositions with biocides for plant protection.

Author Biographies

Volodumur Shvets, Department of Physical Chemistry of Fossil Fuels InPOCC, National Academy of Sciences of Ukraine

Ph.D student

Olena Karpenko, Department of Physical Chemistry of Fossil Fuels InPOCC, National Academy of Sciences of Ukraine

D.Sci., Head of Division of Chemistry and Biotechnology

Ilona Karpenko, Department of Physical Chemistry of Fossil Fuels InPOCC, National Academy of Sciences of Ukraine

Junior Researcher

Volodymyr Novikov, Lviv Polytechnic National University

Prof., D.Sci., Head of the Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology

Vira Lubenets, Lviv Polytechnic National University

D.Sci., Professor at the Department of Technology of Biologically Active Substances, Pharmacy and Biotechnology

References

Hojgard S, Faruk K. Antibiotic resistance. Why is the problem so difficult to solve? Infect Ecol Epidemiol. 2012 Aug;2. DOI 10.3402/iee.v2i0.18165

Lubenets VІ. Thiosulfonates: synthesis and properties. Ukrayins'kyy Khimichnyy Zhurnal. 2003;69(3):109-17.

Lubenets V, Vasyljuk S, Monka N, Bolibrukh K, Komarovska-Porokhnyavets O, Baranovych D, et al. Synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid S-esters. Saudi Pharmaceut J. 2017;25(2):266-74. DOI 10.1016/j.jsps.2016.06.007

Reddy B, Kawamori T, Lubet R, Steele V, Kelloff G, Rao CV. Chemopreventive effect of S-methylmethane thiosulfonate and sulindac administered together during the promotion/progression stages of colon carcinogenesis. Carcinogenesis. 1999 Aug;20(8):1645-48. DOI 10.1093/carcin/20.8.1645

Galabova D, Tuleva B, Spasova D. Permeabilization of Yarrowia lipolytica cells by triton X-100. Enzyme Microbial Tech-nol. 1996 Jun;18:18-22. DOI 10.1016/0141-0229(96)00063-4

Pacwa-Płociniczak M, Płaza GA , Piotrowska-Seget Z, Cameotra SS. Environmental applications of biosurfactants: recent advances, Int J Mol Sci. 2011 Jan 18;12(1):633-54. DOI 10.3390/ijms12010633

Lubenets V, Karpenko O, Ponomarenko M, Zahoriy G, Krychkovska A, Novikov V. Development of new antimicrobial compositions of thiosulfonate structure. Chem Chem Technol. 2013;7(2):119-24.

Sotirova A, Avramova T, Stoitsova S, Lazarkevich I, Lubenets V, Karpenko E, et al. The importance of rhamnolipid-biosurfactant induced changes in bacterial membrane lipids of Bacillus subtilis for the antimicrobial activity of thiosul-fonates. Curr Microbiol. 2012 Nov;65(5):534-41. DOI 10.1007/s00284-012-0191-7

Lubenets V, Vasylyuk S, Baranovych D, Novikov V. The plant protection remedies of thiosulfonate type. Chem Agricult Environ. 2007;8:163-7.

Karpenko EV, Pokynbroda TYa, Makytra RH, Palchykova EYa. Optimal methods for isolating biogenic surface-active rhamnolipids. Zhurnal Obshhej Himii, 2009;12:2011-14.

Segy J, Methods of soil microbiology. Moscow: Kolos; 1983. 296 p.

Sachdev DP, Cameotra SS. Biosurfactants in agriculture. Appl Microbiol Biotechnol. 2013 Feb;97(3):1005-16. DOI 10.1007/s00253-012-4641-8

Sotirova AV, Spasova DI, Galabova DN, Karpenko E, Shulga A. Rhamnolipid-biosurfactant permeabilizing effects on Gram-positive and Gram-negative bacterial strains. Curr Microbiol. 2008 Jun;56(6):639-44. DOI 10.1007/s00284-008-9139-3

Sotirova A, Avramova T, Lazarkevich I, Galabova D. Antimicrobial potential of selected thiosulfonates-based biocides and biosurfactants against bacteria and fungi. Reports BAS. Biologie Microbiologie. 2010;63(9):1307-14.

Published

2017-12-19

How to Cite

1.
Shvets V, Karpenko O, Karpenko I, Novikov V, Lubenets V. Antimicrobial Action of Compositions Based on Thiosulfonates and Biosurfactants on Phytopathogens. Innov Biosyst Bioeng [Internet]. 2017Dec.19 [cited 2024Nov.24];1(1):43-8. Available from: https://ibb.kpi.ua/article/view/112895

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