The Role of reactive Oxygen Species in the Implementation of the Anti-Tumor Effect of Nanocomplexes Based on GdEuVO4 Nanoparticles and Cholesterol
DOI:
https://doi.org/10.20535/ibb.2024.8.2.295581Keywords:
nanocomplexes, Ehrlich adenocarcinoma cells, reactive oxygen species, apoptosis, necrosis, vanadium compoundsAbstract
Background. An experimental study of the antitumor effect of nanocomplexes (NCs) consisting of GdYEuVO4 nanoparticles and cholesterol indicates their potential use in oncological practice. The mechanism of the antitumor effect of NCs may be associated with the formation of reactive oxygen species (ROS), leading to subsequent tumor cell death.
Objective. To study the pro-oxidant and antitumor properties of NCs consisting of GdYEuVO4 nanoparticles and cholesterol in an in vitro system.
Methods. Experiments were performed on Ehrlich ascites carcinoma (EAC) cells, which were introduced intraperitoneally into BALB/c mice. On the 7th day of EAC development, cells were isolated and treated with NCs for 3 hours in vitro. Untreated cells served as the control. The formation of intracellular ROS was quantified by flow cytometry using the Fluorometric Intracellular ROS Kit. The metabolic activity of EAC cells was assessed using the colorimetric MTT test. The number of cells undergoing apoptosis or necrosis was evaluated using flow cytometry and the FITC Annexin V Apoptosis Detection Kit I.
Results. Incubation of EAC cells with NCs resulted in more than a 3-fold increase in ROS formation compared to the control. NCs also caused almost a two-fold inhibition of the metabolic activity of EAC cells, accompanied by a 25% decrease in the number of viable EAC cells. It was shown that NCs are unique compounds capable of simultaneously inducing several types of cell death, with necrosis being the predominant mode of tumor cell death after NC treatment.
Conclusions. The cytotoxic effect of NCs on tumor cells is mediated through their pro-oxidant properties. These results can be considered when developing new cancer therapy strategies.
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