Viability of Nodule Bacteria Bradyrhizobium japonicummon Soybean Seeds by Treatment With Fungicides During Extended Storage
DOI:
https://doi.org/10.20535/ibb.2023.7.4.278905Keywords:
Bradyrhizobium japonicum, viability of rhizobia, seed treatment fungicide, fungicide Fever, fungicide Maxim X, soybean seedsAbstract
Background. With the increase in soybean cultivation areas, inoculants are becoming increasingly sought after. They are not only compatible with the original preparations for seed treatment of soybean but also offer the possibility of applying them for several days or even months before sowing into the soil.
Objective. The viability of new strains of nodule bacteria Bradyrhizobium japonicum (strain PC07 and strain B78) was investigated on the surface of soybean seeds treated with fungicides Fever and Maxim XL during the extended storage of inoculated seeds. Additionally, their ability for nitrogen fixation under symbiotic conditions was evaluated.
Methods. Сultivation, serial dilution method, determination of bacterial titer of inoculants, quantification of colony forming units (CFU), gas chromatography.
Results. In laboratory conditions, on the soybean seeds of Almaz and Vasylkivska varieties, the viability of the nodule bacteria B. japonicum PC07 and B78, which are more resistant to the active substances of these fungicides in pure culture, decreased. This reduction was dependent on the storage period, the fungicide used, and the strain of inoculant. One day after inoculation on the seeds without the use of fungicide, 68.6–75.4% CFU of the initial number was retained. Seeds treated with the fungicide Fever contained 36.5–38.3% CFU, and those treated with Maxim XL contained 26.2–27.0% CFU. The use of inoculants based on fungicide-resistant strains of B. japonicum PC07 and B78 also provided high cell viability – 2.41–2.8×106 (on the seeds without treatment), 1.40–1.70×106 (with Fever) and 0.8–1.17×106 (with Maxim XL) CFU/seed after 5 days of storage. The nitrogen-fixing activity of root nodules in soybean plants of Almaz and Vasylkivska varieties, whose seeds were treated with Fever, decreased on both varieties by 18.4–22.4% and 32.1–39.5%, respectively and for treatment with Maxim XL, the reduction was 24.5–33.7% and 47.7–75.2%, respectively, during the storage of seeds for 5 and 7 days before sowing, compared to control variants (seeds without fungicide treatment).
Conclusions. The utilization of fungicide-resistant strains of B. japonicum PC07 and B78 for bacterization of soybean seeds treated with fungicides Fever and Maxim XL provides a high inoculation titer during 5 days of storage. Viability of microbial cells on seeds treated with fungicides significantly diminishes during storage for more than 7–14 days. Insufficient titer of CFU of B. japonicum strains PC07 and B78 on the seed surface after 14 days of storage can considerably impede the efficacy of biopreparations. Therefore, it is crucial to seek substances that can enhance the resistance of rhizobia on seeds to the adverse effects of fungicide seed treatment, ensuring a longer period of their viability.
References
Munkvold GP, Watrin C, Scheller M, Zeun R, Olaya G. Benefits of chemical seed treatments on crop yield and quality. In: Gullino M, Munkvold G, editors. Global perspectives on the health of seeds and plant propagation material. Pant pathology in the 21st century. 6. Dordrecht: Springer; 2014. p. 89-103. DOI: 10.1007/978-94-017-9389-6_7
Gaban G, Rodrigues JAS, da Silva GZ, Machado CG, Carneiro LC, Cruz SCS. Treatment of soybean seeds to control pathogenic fungi and maintain physiological quality. Científica. 2020 Sept;48(3):243-9. DOI: 10.15361/1984-5529.2020v48n3p243-249
Sammauria R, Kumawat S, Kumawat P, Singh J, Jatwa TK. Microbial inoculants: potential tool for sustainability of agricul-tural production systems. Arch Microbiol. 2020 Jan;202(4):677-93. DOI: 10.1007/s00203-019-01795-w
Kobak SY. High-quality inoculation is the first step to a high yield. Ahronomiia Sohodni. 2016 Feb;1-2:53-5.
Hartley EJ, Gemell LG, Deaker R. Some factors that contribute to poor survival of rhizobia on preinoculated legume seed. Crop Pasture Sci. 2012 Dec;63(9):858-65. DOI: 10.1071/CP12132
Imfelda G, Vuilleumierb S. Measuring the effects of pesticides on bacterial communities in soil: a critical review. Eur J Soil Biol. 2012 Mar-Apr;49:22-30. DOI: 10.1016/j.ejsobi.2011.11.010
Deshwal VK, Singh SB, Kumar P, Chubey A. Rhizobia unique plant growth promoting rhizobacteria: a review. Int J Life Sci. 2013 May;2(2):74-86.
Ahemad M, Khan MS. Effect of pesticides on plant growth promoting traits of greengram-symbiont, Bradyrhizobium sp. strain MRM6. Bull Environ Contam Toxicol. 2011 Apr;86(4):384-388. DOI: 10.1007/s00128-011-0231-1
Mishra G, Kumar N, Giri K, Pandey S. In vitro interaction between fungicides and beneficial plant growth promoting Rhi-zobacteria. Afr J Agricult Res. 2013 Nov;8(45):5630-3.
Pereira CE, Moreira FM de S, Oliveira JA, Caldeira CM. Compatibility among fungicide treatments on soybean seeds through film coating and inoculation with Bradyrhizobium strains. Agronomy. 2010 Oct;32(4):585-9. DOI: 10.4025/actasciagron.v32i4.5756
Keum YS, Lee HR, Kim JH. Effects of pesticides on the bacterial production of pyrrolnitrin. J Agricult Food Chem. 2010 Apr;58(9):5531-7. DOI: 10.1021/jf904195j
Temprano FJ, Albareda M, Camacho M, Daza A, Santamaria C, Rodriguez-Navarro DN. Survival of several Rhizobi-um/Bradyrhizobium strains on different inoculant formulations and inoculated seeds. Int Microbiol. 2002 May;5:81-6. DOI: 10.1007/s10123-002-0067-y
Gaind S, Rathi MS, Kaushik BD, Nain L, Verma OP. Survival of bio-inoculants on fungicides-treated seeds of wheat, pea and chickpea and subsequent effect on chickpea yield. J Environ Sci Health B. 2007 Sept;42(6):663-8. DOI: 10.1080/03601230701465759
Romero-Perdomo FA, Camelo M, Bonilla R. Response of Bradyrhizobium japonicum to alginate in presence of pelleted fungicides on soybean seeds. Revista U D C A Actualidad Divulgaciуn Cientifica. 2015 July-Dec;18(2):359-64. DOI: 10.31910/rudca.v18.n2.2015.161
Revellin C, Leterme P, Catroux G. Effect of some fungicide seed treatments on the survival of Bradyrhizobium japonicum and on the nodulation and yield of soybean. Biol Fertil Soils. 1993 Aug;16:211-4. DOI: 10.1007/BF00361410
Campo RJ, Araujo RS, Hungria M. Nitrogen fixation with the soybean crop in Brazil: compatibility between seed treat-ment with fungicides and Bradyrhizobial inoculants. Symbiosis. 2009 Feb;48(1-3):154-63. DOI: 10.1007/BF03179994
Tariq M, Hameed S, Shahid M, Yasmeen T, Ali A. Effect of fungicides and bioinoculants on Pisum sativum. Res Rev J Botan Sci. 2016 May;5(2):36-40.
Deaker R, Hartley E, Gemell G. Conditions affecting shelf-life of inoculated legume seed. Agriculture. 2012 Feb;2(1):38-51. DOI: 10.3390/agriculture2010038
Santos MS, Nogueira MA, Hungria M. Microbial inoculants: reviewing the past, discussing the present and previewing an outstanding future for the use of beneficial bacteria in agriculture. AMB Express. 2019 Dec;9:205. DOI: 10.1186/s13568-019-0932-0
Santos MS, Hungria M, Nogueira MA. Production of PHB and biofilm by Azospirillum brasilense aiming at the develop-ment of liquid inoculants with high performance. Afr J Biotechnol. 2017 Sept;16(37):1855-62. DOI: 10.5897/AJB2017.16162
Hungria M, Loureiro MF, Mendes IC, Campo RJ, Graham PH. Inoculant preparation, production and application. In: Newton WE, editor. Nitrogen fixation in agriculture, forestry, ecology and the environment. Dordrecht: Springer; 2005. p. 223-54. DOI: 10.1007/1-4020-3544-6_11
Marczak M, Mazur A, Koper P, Zebracki K, Skorupska A. Synthesis of rhizobial exopolysaccharides and their importance for symbiosis with legume plants. Genes (Basel). 2017 Dec;8(12):360. DOI: 10.3390/genes8120360
Qurashi AW, Sabri AN. Bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggrega-tion under salt stress. Braz J Microbiol. 2012 Sept;43(3):1183-91. DOI: 10.1590/S1517-83822012000300046
Kukol KP, Vorobey NA, Kots SY. Sensitivity of pure cultures of Bradyrhizobium japonicum to fungicides. Silskohospo-darska Mikrobiolohiia. 2019 Dec;30:20-31. DOI: 10.35868/1997-3004.30.20-31
Vorobey NА, Kukol KP, Kots SY. Fungicides toxicity assessment on Bradyrhizobium japonicum nodule bacteria in pure culture. Mikrobiol Z. 2020 May-June;82(3):45-54. DOI: 10.15407/microbiolj82.03.045
List of pesticides and agrochemicals approved for use in Ukraine in 2023 (as of Jan 12, 2023) [Internet]. 2023 [cited 2023 Feb 17]. Available from: https://agrarii-razom.com.ua/preparations
Antipchuk A, Pilyashenko-Novokhatny A, Yevdokymenko T. Tutorial on Microbiology. Kyiv: University of Ukraine; 2011.
Grodzinsky AM, Grodzinsky DM. Brief handbook on plant physiology. Kyiv: Naukova Dumka; 1964.
Hardy RWF. The acethylene – ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiol. 1968 Aug;43:1185-207. DOI: 10.1104/pp.43.8.1185
Martyniuk S, Oron J, Martyniuk M, Wozniakowska A. Effects of interactions between chemical seed dressings and Brad-yrhizobium japonicum on soybean seeds. Arch Agron Soil Sci. 2002 Sept;48:305-10. DOI: 10.1080/03650340214202
Thies JE, Singleton PW, Bohlool BB. Influence of the size of indigenous rhizobial populations on establishment and symbiotic performance of introduced rhizobia on field-grown legumes. Appl Environ Microbiol. 1991 Jan;57(1):19-28. DOI: 10.1128/aem.57.1.19-28.1991
Singleton PW, Bohlool BB, Nakao PL. Legume response to rhizobial inoculation in the tropics: myth and realities. In: Lai R., Sanchez P, editors. Myths and science of the soils of the tropics. Soil Science Society of America and American Society of Agronomy; 1992. p. 135-55. DOI: 10.2136/sssaspecpub29.c8
Pavlyshche AV, Mamenko TP, Rybachenko LI, Kots SY. Influence of fungicides on the formation, functioning and pe-roxidase activity of root soybean nodules at inoculation by Rhizobia, incubated with lectin. Mikrobiol Z. 2018 Sept-Oct; 80(5):76-89. DOI: 10.15407/microbiolj80.05.076
Kyrychenko OV, Pavlyshche AV, Omelchuk SV, Zhemojda AV, Kots SY. Physiological aspects of the response of soy-bean-rhizobium symbiosis to the action of the fungicides Standak Top and Fever. Ştiinţa Agricolă. 2020;2:259-72.
Araujo RS, Cruz SP, Souchie EL, Martin TN, Nakatani AS, Nogueira MA, et al. Preinoculation of soybean seeds treated with agrichemicals up to 30 days before sowing: technological innovation for large-scale agriculture. Int J Microbiol. 2017 Oct; 2017:5914786. DOI: 10.1155/2017/5914786
Hungria M, Nogueira MA, Campos LJM, Menna P, Brandi F, Ramos YG. Seed pre-Inoculation with Bradyrhizobium as time-optimizing option for large-scale soybean cropping systems. Agron J. 2020 Aug;112:5222-36. DOI: 10.1002/agj2.20392
Martyniuk S. Scientific and practical aspects of legumes symbiosis with root-nodule bacteria. Pol J Agron. 2012;9:1722.
Martyniuk S, Kozieł M, Galazka A. Survival of Rhizobia on seeds, nodulation and growth of soybean as influenced by synthetic and natural seed-applied fungicides. Pol J Agron. 2016 Oct;27:96-9.
Thompson JA. Survival of root-nodule bacteria on inoculated seed. In: Murrell WG, Kennedy IR, editors. Microbiology in action. Letchworth: Research Studies pRess; 1988. p. 67-80.
Sartori FF, Engroff TD, Sanches THG, Soave JM, Pessotto MV, Felisberto G, et al. Potentially harmful effects of seed treatment and pre-inoculation on soybean biological nitrogen fixation and yield. Eur J of Agron. 2023 Jan;142:126660. DOI: 10.1016/j.eja.2022.126660
Deaker R, Roughley RJ, Kennedy IR. Legume seed inoculation technology – a review. Soil Biol Biochem. 2004 Aug;36(8):1275-88. DOI: 10.1016/j.soilbio.2004.04.009
Deaker R, Roughley RJ, Kennedy IR. Desiccation tolerance of Rhizobia when protected by synthetic polymers. Soil Biol Biochem. 2007 Feb;39(2):573-80. DOI: 10.1016/j.soilbio.2006.09.005
Sandini IE, Belani RB, Falbo MK, Pacentchuk F, Huzar-Novakowiski J. Seed treatment and pre-inoculation of soybean: effect of storage period and agrochemicals on the physiological quality of seed and yield. Afr J Agricult Res. 2019 Jan;14(3):151-60. DOI: 10.5897/AJAR2018.13687
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