Cultural and Morphological Features of Trametes versicolor (Polyporaceae) Growth on Wood Hydrolyzes Containing Media
Keywords:Trametes versicolor, Norcrans medium, sawdust extract, Fagales, growth intensification, morphology, growth rate, laccase, peroxidase, tyrosinase
Background. Trametes versicolor is a representative of basidiomycota, whose biologically active compounds are used in medicine and industry, so the search for active producers capable of growing on industrial waste and the study of new ways of intensification of growth and synthesis of metabolites are relevant task.
Objective. Investigation of the effect of Fagales sawdust extracts on growth, morphological characteristics, and induction of synthesis of oxidizing enzymes by macromycetes of the species T. versicolor in surface culture.
Methods. The objects of research were 5 strains of T. versicolor, which were cultivated on the synthetic agarized Norcrans media on Petri dishes. The influence of the sawdust extracts of 3 tree species, which acted as a basis for research media, was studied. The following cultural indicators were investigated: colony size, radial growth rate, and growth rate. The research also covered the study of macromycetes' morphological characteristics and the ability of Fagales sawdust extracts to induce the synthesis of oxidative enzymes.
Results. It is established that growth on media with the addition of birch extract is more intense: the radial growth rate is 11.2–13.6 mm/day, while for beech and oak, the growth rate ranges from 11.6–13.1 and 11.5–12.6 mm/day respectively. The highest growth value on all media is recorded for T. versicolor 353. The value of the growth rate on the medium with birch sawdust extracts varied in the range 15.8–92.1, for medium with beech extracts – 19.4–46.1, with oak extracts – 15.5–46,7, and the highest growth value was recorded during the cultivation of T. versicolor 5299. The beech and oak sawdust extracts intensified the synthesis of laccase, peroxidase, and tyrosinase, which was strongly expressed in T. versicolor 353, so it was chosen for further experiments with submerged cultivation.
Conclusions. Birch, beech, and oak sawdust extracts can be used to increase the accumulation of T. versicolor biomass while beech and oak sawdust extracts are preferable for the intensification of the synthesis of oxidase-type enzymes.
Sergentani AG, Gonou-Zagou Z, Kapsanaki-Gotsi E, Hatzinikolaou DG. Lignocellulose degradation potential of Basidiomycota from Thrace (NE Greece). Int Biodeterior Biodegradation. 2016;114:268-77. DOI: 10.1016/j.ibiod.2016.07.004
Zhang BB, Guan YY, Hu PF, Chen L, Xu GR, Liu L, et al. Production of bioactive metabolites by submerged fermentation of the medicinal mushroom Antrodia cinnamomea: recent advances and future development. Crit Rev Biotechnol. 2019;39(4):541-54. DOI: 10.1080/07388551.2019.1577798
Dudekula UT, Doriya K, Devarai SK. A critical review on submerged production of mushroom and their bioactive metabolites. 3 Biotech. 2020;10(8):337. DOI: 10.1007/s13205-020-02333-y
Li QZ, Zheng YZ, Zhou XW. Fungal immunomodulatory proteins: characteristic, potential antitumor activities and their molecular mechanisms. Drug Discov Today. 2019;24(1):307-14. DOI: 10.1016/j.drudis.2018.09.014
Hsu KD, Wu SP, Lin SP, Lum CC, Cheng KC. Enhanced active extracellular polysaccharide production from Ganoderma formosanum using computational modeling. J Food Drug Anal. 2017;25(4):804-11. DOI: 10.1016/j.jfda.2016.12.006
Varghese R, Dalvi YB, Lamrood PY, Shinde BP, Nair CKK. Historical and current perspectives on therapeutic potential of higher basidiomycetes: an overview. 3 Biotech. 2019;9(10):362. DOI: 10.1007/s13205-019-1886-2
Sandargo B, Chepkirui C, Cheng T, Chaverra-Muñoz L, Thongbai B, Stadler M, et al. Biological and chemical diversity go hand in hand: Basidiomycota as source of new pharmaceuticals and agrochemicals. Biotechnol Adv. 2019;37(6):107344. DOI: 10.1016/j.biotechadv.2019.01.011
Pilafidis S, Diamantopoulou P, Gkatzionis K, Sarris D. Valorization of agro-industrial wastes and residues through the production of bioactive compounds by macrofungi in liquid state cultures: growing circular economy. Appl Sci. 2022;12(22):11426. DOI: 10.3390/app122211426
Cateni F, Gargano ML, Procida G, Venturella G, Cirlincione F, Ferraro V. Mycochemicals in wild and cultivated mushrooms: nutrition and health. Phytochem Rev. 2022;21(2):339-83. DOI: 10.1007/s11101-021-09748-2
Yadav D, Negi PS. Bioactive components of mushrooms: Processing effects and health benefits. Food Res Int. 2021;148:110599. DOI: 10.1016/j.foodres.2021.110599
Fabros J, Dulay R, De Leon A, Kalaw S, Reyes R. Distribution, cultivation, nutritional composition, and bioactivities of Lentinus (Polyporaceae, Basidiomycetes): A review. Curr Res Environ Appl Mycol. 2022;12(1):170-219. DOI: 10.5943/cream/12/1/13
Liu D, Chen YQ, Xiao XW, Zhong RT, Yang CF, Liu B, et al. Nutrient properties and nuclear magnetic resonance-based metabonomic analysis of macrofungi. Foods. 2019;8(9):397. DOI: 10.3390/foods8090397
Yap HYY, Ariffeen Rosli MF, Tan SH, Kong BH, Fung SY. The wound healing potential of Lignosus rhinocerus and other ethno-myco wound healing agents. Mycobiology. 2023;51(1):1-15. DOI: 10.1080/12298093.2022.2164641
Hapuarachchi KK, Karunarathna SC, Xu XH, Dutta AK, Phengsintham P, Hyde KD, et al. A review on bioactive compounds, beneficial properties and biotechnological approaches of Trametes (Polyporaceae, Polyporales) and a new record from Laos. Chiang Mai J Sci. 2021;48(3):674-98.
Veena SS, Pandey M. Physiological and cultivation requirements of Trametes versicolor, a medicinal mushroom to diversify Indian mushroom industry. Indian J Agric Sci. 2012;82(8):672-5.
Habtemariam S. Trametes versicolor (Synn. Coriolus versicolor) polysaccharides in cancer therapy: targets and efficacy. Biomedicines. 2020;8(5):135. DOI: 10.3390/biomedicines8050135
Hernández Mendieta E, Ramos MA, Miranda AS, Granados CR. Evaluation of crude extracts of Trametes versicolor (L.:Fr.) Pilát to control of phytopathogenic fungi. Int J Plant Res. 2019;9:8-13. DOI: 10.5923/j.plant.20190901.02
Jo WS, Kang MJ, Choi SY, Yoo YB, Seok SJ, Jung HY. Culture conditions for mycelial growth of Coriolus versicolor. Mycobiology. 2010;38(3):195. DOI: 10.4489/MYCO.2010.38.3.195
Elkhateeb WA, Elnahas MO, Thomas PW, Daba GM. Trametes Versicolor and Dictyophora Indusiata champions of Medicinal mushrooms. Open Access J Pharm Res. 2020;4(1). DOI: 10.23880/OAJPR-16000192
Meng F, Lin Y, Hu L, Feng W, Su P, Wu L. The therapeutic effect of Coriolus versicolor fruiting body on STZ-induced ICR diabetic mice. J Healthc Eng. 2022;2022:1-9. DOI: 10.1155/2022/7282453
Dulay RMR, Alcazar AA, Kalaw SP, Reyes RG, Cabrera EC. Nutritional and physical requirements for mycelial growth and basidiocarp production of Trametes elegans from the Philippines. Asian J Agric Biol. 2021;2021(1). DOI: 10.35495/ajab.2020.06.339
Rameshaiah GN, Jagadish Reddy ML. Applications of ligninolytic enzymes from a white-rot fungus Trametes versicolor. Univers J Environ Res Technol. 2015;5(1):1-7.
Enzim. Plant of microbiological synthesis preparations manufacture [Internet]. Enzim. 2023 [cited 2023 Feb 15]. Availabe from: https://enzim.ua/index.php
Sagar S, Thakur M, Sharma I, Tripathi A. Optimization of mycelia growth parameters forWild white rot fungi Trametes elegans and Trametes versicolor. Asia Life Sci. 2020;12:5-14.
Nguyen BTT, Le VV, Nguyen HTT, Nguyen LT, Dong TA, Xuan NN. Successful rescue of wild trametes versicolor strains using sawdust and rice husk-based substrate. Pak J Biol Sci. 2021;24(3):374-82. DOI: 10.3923/pjbs.2021.374.382
Klechak IR, Bisko NA, Poyedinok NL, Antonenko LO. The growth mechanisms of the promising research subjects of biotechnology-basidiomycetes mushrooms of the genus Coriolus on agar mediums. Naukovi Visti NTUU KPI. 2008;6:100-7.
Vishvakarma R, Vimal A, Mishra A, Sharma P, Gaur VK. Trametes versicolor (L.) Lloyd as a source of thermostable serine protease: production and characterization. Indian J Exp Biol. 2022;60(9). DOI: 10.56042/ijeb.v60i09.65147
Nguyen BTT, Le VV, Nguyen HTT, Nguyen LT, Tran TTT, Ngo NX. Nutritional requirements for the enhanced mycelial growth and yield performance of Trametes versicolor. J Appl Biol Biotechnol. 2021;9(1):1-17. DOI: 10.7324/JABB.2021.9101
Langenaeken NA, De Schepper CF, De Schutter DP, Courtin CM. Carbohydrate content and structure during malting and brewing: a mass balance study. J Inst Brew. 2020;126(3):253-62. DOI: 10.1002/jib.619
Intan Kailaku S, Nur Alam Syah A, Risfaheri R, Setiawan B, Sulaeman A. Carbohydrate-electrolyte characteristics of coconut water from different varieties and its potential as natural isotonic drink. Int J Adv Sci Eng Inf Technol. 2015;5(3):174. DOI: 10.18517/ijaseit.5.3.515
Saunders RM. Rice bran: Composition and potential food uses. Food Rev Int. 1985;1(3):465-95. DOI: 10.1080/87559128509540780
Zilic S, Milasinovic M, Terzic D, Barac M, Ignjatovic-Micic D. Grain characteristics and composition of maize specialty hybrids. Spanish J Agric Res. 2011;9(1):230. DOI: 10.5424/sjar/20110901-053-10
Bondarchuk AA, Vermenko YY, Furdyha MM. Consumer quality of potato varieties selected by the Institute for Potato Research of the National Academy of Agrarian Sciences. Kartoplyarstvo. 2016;43:94-110.
Ivanova T. Effects of membranotropic microfertilizers to grow the mycelium of Lentinula edodes. J Microbiol Biotechnol Food Sci. 2019;9(3):605-9. DOI: 10.15414/jmbfs.2019/184.108.40.2065-609
Bisko N, Lomberg M, Mykchaylova O, Mytropolska N. IBK Mushroom Culture Collection. Version 1.2. The IBK Mu-shroom Culture Collection of the M.G. Kholodny Institute of Botany [Data file]. M.G. Kholodny Institute of Botany; 2020 [cited 2023 Feb 15]. Available from: https://doi.org/10.15468/dzdsqu
Plotnikov EV, Glukhova LB, Sokolyanskaya LO, Karmachuk OV, Solioz M. Effect of tree species on enzyme secretion by the shiitake medicinal mushroom, Lentinus edodes (Agaricomycetes). Int J Med Mushrooms. 2016;18(7):637-44. DOI: 10.1615/IntJMedMushrooms.v18.i7.90
Bukhalo AS. Higher edible basidiomycetes in pure culture. Kyiv: Naukova Dumka; 1988. 144 p.
Dzigun LP. Features of the wood-destroying basidiomycete Laetiporus sulphureus (Bull.:Fr.) Murrill in culture. Ukr Bot J. 2004;61(1):100-5.
Günther A, Schulze ED, Purahong W, Gminder A, Tanunchai B, Schneider H, et al. Biodiversity and conservation of Basidiomycetes: The importance of urban areas and associated. Artenschutz Rep. 2022;46:1–11.
Gautam AK. Notes on wood rotting fungi from India (1): Trametes versicolor - The Turkey Tail. J New Biol Reports. 2013;2(2):67-70.
Montoya S, Patiño A, Sánchez ÓJ. Production of lignocellulolytic enzymes and biomass of Trametes versicolor from agro-industrial residues in a novel fixed-bed bioreactor with natural convection and forced aeration at pilot scale. Processes. 2021;9(2):397. DOI: 10.3390/pr9020397
Schritt H, Vidi S, Pleissner D. Spent mushroom substrate and sawdust to produce mycelium-based thermal insulation composites. J Clean Prod. 2021;313:127910. DOI: 10.1016/j.jclepro.2021.127910
Irbe I, Loris GD, Filipova I, Andze L, Skute M. Characterization of self-growing biomaterials made of fungal mycelium and various lignocellulose-containing ingredients. Materials (Basel). 2022;15(21):7608. DOI: 10.3390/ma15217608
Miazek K, Remacle C, Richel A, Goffin D. Beech wood Fagus sylvatica dilute-acid hydrolysate as a feedstock to support Chlorella sorokiniana biomass, fatty acid and pigment production. Bioresour Technol. 2017;230:122-31. DOI: 10.1016/j.biortech.2017.01.034
Chauhan K, Sharma KR, Dutt B. Variation in hot and cold water soluble extractive content in gymnosperms from Western Himalayas. J Pharmacogn Phytochem. 2020;9(5):1151-4. DOI: 10.22271/phyto.2020.v9.i5p.12383
Enman J, Hodge D, Berglund KA, Rova U. Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes. J Chem Technol Biotechnol. 2012;87(7):903-7. DOI: 10.1002/jctb.3697
Tanesaka E, Takeda H, Yoshida M. Phenol-oxidizing enzyme expression in lentinula edodes by the addition of sawdust extract, aromatic compounds, or copper in liquid culture media. Biocontrol Sci. 2013;18(3):143-9. DOI: 10.4265/bio.18.143
Lomberg ML, Solomko EF. Growth of macromycetes cultures on agarized nutrient media and dense substrates. In: Vasser S, editor. Biological properties of medicinal macromycetes in a culture. Kyiv: Altpres; 2012. p. 345-71.
Koutrotsios G, Zervakis GI. Comparative examination of the olive mill wastewater biodegradation process by various wood-rot macrofungi. Biomed Res Int. 2014;2014:1-14. DOI: 10.1155/2014/482937
Velásquez-Riaño M, Carvajal-Arias CE, Rojas-Prieto NL, Ausecha-García SA, Vera-Díaz MÁ, Meneses-Sánchez JS, et al. Evaluation of a mixed simultaneous vinasse degradation treatment using Komagataeibacter kakiaceti GM5 and Trametes versicolor DSM 3086. Ecotoxicol Environ Saf. 2018;164:425-33. DOI: 10.1016/j.ecoenv.2018.08.037
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