Use of Highly Dispersed Silica in Biotechnology of Complex Probiotic Product Based on Bifidobacteria
Keywords:bifidobacteria, highly dispersed silica, taxonomic position, immobilization, healthcare products
Background. The probiotics immobilization technology is one of the most effective ways for controlled and continuous delivery of viable cells into the intestine. It is well known that multifaceted physiological roles of bifidobacteria are to normalize and stabilize the microbiocenosis, to form intestine colonization resistance, to synthesis amino acids, proteins and vitamins, to maintain non-specific resistance of the organism and so all. Such a wide range of positive effects on the macroorganism allows us to consider bifidobacteria as a basis for functional immobilized healthcare products development.
Objective. Taxonomic position determination of the Bifidobacterium longum strain selected for immobilization, study of the viability of this bifidobacteria strain in a complex probionic product based on highly dispersed silica in simulated gastrointestinal tract's conditions and after freeze-drying.
Methods. The production strain Bifidobacterium longum IMV B-7165 from the Institute of Food Resources of the National Academy of Agrarian Sciences of Ukraine collection of industrial strains has been used in the study. It was isolated from the healthy human infant's gastrointestinal tract.
Commonly used bioinformatics, microbiological, biotechnological and statistical methods have been applied.
Results. The best alignments for the sequence of bifidobacteria isolate "4202" 16S rRNA (it was previously deposited as Bifidobacterium longum IMV B-7165) and classic dendrograms based on these results were performed. According to the results of microscopic studies of samples of microorganisms with highly dispersed silica products ("Enterosgel", "Sillard P" and "Toxin.Net") it was found that the immobilization of the Streptococcus thermophilus and bifidobacteria cultures did not differ fundamentally. To study the immobilization effect on the bifidobacteria preservation and properties the following carriers were used: "Enterosgel", "Toxin.NET" and "Sillard P". The survival of immobilized bifidobacteria was further studied in simulated gastrointestinal conditions: immobilized cells are better protected from acid and bile, although with increasing acidity, survival decreases in both control and immobilized cells.
Conclusions. The taxonomic position of a bifidobacterial isolate from the healthy human infants used in immobilization studies was clarificated (Bifidobacterium animalis subsp lactis). Under the simulated conditions of the upper gastrointestinal tract in the case of acid and bile impact, the best survival was demonstrated by immobilized cultures of bifidobacteria together with the Enterosgel sorbent (a content of 10% by weight of the culture). The survival of immobilized preparations after freeze-drying was slightly reduced in the case of immobilization on the "Enterosgel" and "Toxin.NET" samples of enterosorbents (a content from 15% to 25% by weight of the culture). The best results were observed in the case of immobilization of bifidobacteria with 5% content of the "Toxin.NET" enterosorbent (enterosgel + inulin).
Berg G, Rybakova D, Fischer D, Cernava T, Vergès MC, Charles T, et al. Microbiome definition re-visited: old concepts and new challenges. Microbiome. 2020 Jun 30;8(1):103. DOI: 10.1186/s40168-020-00875-0
Kho ZY, Lal SK. The human gut microbiome – a potential controller of wellness and disease. Front Microbiol. 2018 Aug 14;9:1835. DOI: 10.3389/fmicb.2018.01835
Monsour HP Jr, Quigley EM. The microbiome: what will the future hold. Semin Liver Dis. 2016 Sep;36(4):354-9. DOI: 10.1055/s-0036-1594009.
Starovoitova SO. Modern aspects of technology of immobilized probiotics. Biotechnologia Acta. 2012;5(4):9-20.
Iravani S, Korbekandi H, Mirmohammadi SV. Technology and potential applications of probiotic encapsulation in fermented milk products. J Food Sci Technol. 2015 Aug;52(8):4679-96. DOI: 10.1007/s13197-014-1516-2
de Paiva e Silva KK, de Souza Queirós M, Ribeiro APB, Gigante ML. Modified milk fat as encapsulating material for the probiotic microorganism Lactobacillus acidophilus LA3. Int Dairy J. 2022;125:105237. DOI: 10.1016/j.idairyj.2021.105237
Babynets O.M. Properties of probiotics immobilized on enterosorbents after low-temperature storage. Bull Probl Biol Med. 2012;4(1):72-8.
Haffner FB, Diab R, Pasc A. Encapsulation of probiotics: insights into academic and industrial approaches. AIMS Mater Sci. 2016;3(1):114-36. DOI: 10.3934/matersci.2016.1.114
Kozlovska HV, Danylenko SG, Ibatullina FZ. Selection of protective media in the process of lyophilization of strains of bifidobacteria and lactobacilli. Sci J Nat Univ Life Environ Sci Ukr. 2012; 72(4):30-3.
Gorbyk PP, Pentyuk OO, Shtatko OI. Prospects for the creation of combined drugs based on highly dispersed silica. Nanosyst Nanomater Nanotechnol. 2008;6(1):315-330.
Kim SE, Zhang L, Ma K, Riegman M, Chen F, Ingold I, et al. Ultrasmall nanoparticles induce ferroptosis in nutrient-deprived cancer cells and suppress tumour growth. Nat Nanotechnol. 2016;11(11):977-85. DOI: 10.1038/nnano.2016.164
Selvarajan V, Obuobi S, Ee PLR. Silica nanoparticles—a versatile tool for the treatment of bacterial infections. Front Chem. 2020 Jul 15;8:602. DOI: 10.3389/fchem.2020.00602
Zaytsev V. Complexing silicas. Synthesis, graft structure and surface chemistry. Folio: Kharkiv; 1997. 233 р.
Danylenko S, Romanchuk I, Marynchenko L, Kryzhska T, Nizhelska O, Potemska O, et al. Immobilization of probiotic cultures with enterosorbents based on highly dispersed silica. J Microbiol Biotechnol Food Sci. 2021;11(2):1-4. DOI: 10.15414/jmbfs.3334
Danylenko S. Properties of microorganisms strains L. paracasei ssp. paracasei AND B. longum subsp. suis. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies Ser Veterinary Sciences. 2013;15(1):296-301.
Knysh OV, Isaienko OI, Babych YM, Kompaniets AM, Pakhomov OV, Polyanska VP, et al. Antimicrobial activity of bifidobacteria derivatives after storage in a frozen state. Probl Cryobiol Cryomed. 2015;28(3):267-86. DOI: 10.15407/cryo28.03.237
BLAST: Basic Local Alignment Search Tool [Internet]. Blast.ncbi.nlm.nih.gov. 2022 [cited 2021 Nov 23]. Available from: https://blast.ncbi.nlm.nih.gov/
Boke H, Aslim B, Alp G. The role of resistance to bile salts and acid tolerance of exopolysaccharides (EPSS) produced by yogurt starter bacteria. Arch Biol Sci. 2010;62(2):323-8. DOI: 10.2298/abs1002323b
Guergoletto KB, Magnani M, Martin JS, Andrade CGTdJ, Garcia S. Survival of Lactobacillus casei (LC-1) adhered to prebiotic vegetal fibers. Innov Food Sci Emerg Technol. 2010;11(2):415-21. DOI: 10.1016/j.ifset.2009.11.003
Feklysova LV, Meskyna EO. A new generation of sorbed bifid probiotics in pediatric practice. Almanac Clin Med. 2005;8(1):329-38.
Ding WK, Shah NP. Acid, bile, and heat tolerance of free and microencapsulated probiotic bacteria. J Food Sci. 2007;72(9):446-50. DOI: 10.1111/j.1750-3841.2007.00565.x
Ding WK, Shah NP. An improved method of microencapsulation of probiotic bacteria for their stability in acidic and bile conditions during storage. J Food Sci. 2009;74(2):53-60. DOI: 10.1111/j.1750-3841.2008.01030.x
Ding WK, Shah NP. Effect of various encapsulating materials on the stability of probiotic bacteria. J Food Sci. 2009;74(2):100-7. DOI: 10.1111/j.1750-3841.2009.01067.x
Jayalalitha V, Balasundaram B, Palanidorai R. In vitro assessment of microencapsulated probiotic beads. Int J Agricult Res Rev. 2012;2(1):1-6.
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