Antioxidant activity of petunias with the heterologous ribonuclease ZRNase II gene infected with tobacco mosaic virus

Authors

DOI:

https://doi.org/10.20535/ibb.2022.6.1.254464

Keywords:

transgenic plants, genetic transformation, petunia, tobacco mosaic virus, stress reactions, lipid peroxidation, antioxidant activity

Abstract

Background. Constant changes in environmental conditions cause the development of stress reactions in plants. Under conditions of moderate intensity and temporary action of the stress factor, the strengthening of protective systems and the mobilization of energy resources take place. However, if the stress factor has a long-term effect, the cells begin the processes of lipid peroxidation (LPO), inhibition of energy production and reduction of protein synthesis with its subsequent destruction. Under conditions of excessive stress, there is a balance between antioxidant activity (AOA) and LPO, which is necessary to maintain normal cell function. Oxidation intermediates can serve as inducers and mediators of stress. Phytovirus infection can lead to pathological changes in the body of a plant. The progression of the infectious process in the body of the affected plant is associated with stress reactions and disruption of its normal viability.

Objective. We are aimed to assess the degree of progress of stress reactions caused by biotic stressors in control and transgenic (with ZRNase II gene) petunia plants.

Methods. Tobacco mosaic virus (TMV) was used to infect petunia plants. The degree of progress of stress reactions in transgenic petunia plants with the ZRNase II gene before and after infection with TMV was studied by POL and AOA indicators. Two genetically distinct lines of petunia (M1 and P5) were used to obtained transgenic plants. To assess the progress of LPO, the accumulation of initial and final products (diene conjugates and malonic dialdehyde) was determined.

Results. After the plants transformation, changes in the content of LPO products in leaf tissues were observed. Transgenic plants had a 10–15% higher content of LPO products, which may indicate that the transformation, in some cases, can lead to the progress of stress reactions in plants. Infection with TMV has contributed to the intensification of processes related to the protection of plants from the effects of negative factors. Studies of total AOA have shown that transgenic plants after infection had significantly higher levels (18–30%) of AOA compared with controls, which may be evidence of their increased viability under stress.

Conclusions. The positional effect of T-DNA incorporation in genetic transformation may be a stressor for the plant. Transgenic lines differ in terms of LPO and AOA from non-transgenic lines and from each other. After infection with TMV, a 4-fold decrease in AOA was observed in the plants. Effective expression of the ZRNase II gene helps to reduce viral load in certain lines. Lines M1.2 and P5.3 are of greatest interest for further virological studies, as their AOA activity was 18–30% higher than in control plants, which may indicate resistance to viral infection.

References

Atkinson NJ, Urwin PE. The interaction of plant biotic and abiotic stresses: from genes to the field, J Exp Bot. 2012 Jun;63(10):3523-43. DOI: 10.1093/jxb/ers100

Qamer Z, Chaudhary MT, Du X, Hinze L, Azhar MT. Review of oxidative stress and antioxidative defense mechanisms in Gossypium hirsutum L. in response to extreme abiotic conditions. J Cotton Res. 2021;4(1):9. DOI: 10.1186/s42397-021-00086-4

Kowalczewski PŁ, Radzikowska D, Ivanišová E, Szwengiel A, Kačániová M, Sawinska Z. Influence of abiotic stress factors on the antioxidant properties and polyphenols profile composition of green barley (Hordeum vulgare L.). Int J Mol Sci. 2020 Jan 8;21(2):397. DOI: 10.3390/ijms21020397

Nicky J. The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot. 2012 Jun;63(10):3523-43. DOI: 10.1093/jxb/ers100

He M, He CQ, Ding NZ. Abiotic stresses: general defenses of land plants and chances for engineering multistress tolerance. Front Plant Sci. 2018 Dec 7;9:1771. DOI: 10.3389/fpls.2018.01771

Vahdati K, Leslie C, editors. Abiotic stress – plant responses and applications in agriculture. InTechOpen; 2013. 410 p. DOI: 10.5772/45842

Kusvuran S, Kiran S, Ellialtioglu SS. Antioxidant enzyme activities and abiotic stress tolerance relationship in vegetable crops. In: Shanker AK, Shanker C, editors. Abiotic and biotic stress in plants. InTechOpen; 2016. DOI: 10.5772/62235

Dumanović J, Nepovimova E, Natić M, Kuča K, Jaćević V. The significance of reactive oxygen species and antioxidant defense system in plants: a concise overview. Front Plant Sci. 2021 Jan 6;11:552969. DOI: 10.3389/fpls.2020.552969

Xie X, He Z, Chen N, Tang Z, Wang Q, Cai Y. The roles of environmental factors in regulation of oxidative stress in plant. Biomed Res Int. 2019 May 8;2019:9732325. DOI: 10.1155/2019/9732325

Lapikova VP, Gaivorovskaya LM, Averyanov AA. Possible participation of reactive oxygen species in double induction of anti-infectious plant reactions. Physiol Plants 2000;47(1):161-2.

Mittler R. Abiotic stress, the field environment and stress combination. Trends Plant Sci. 2006 Jan;11(1):15-9. DOI: 10.1016/j.tplants.2005.11.002

Sibgatullina GV, Khaertdinova LR, Gumerova EA, Akulov AN, Kostyukova YA, Nikonorova NA, et al. Methods for determining the redox status of cultivated plant cells. Kazan: Kazan Federal University; 2011. 61 p.

Anwar A, Kim JK. Transgenic breeding approaches for improving abiotic stress tolerance: recent progress and future perspectives. Int J Mol Sci. 2020 Apr;21(8):2695. DOI: 10.3390/ijms21082695

Waqas Muhammad Ahmed, Kaya Cengiz, Riaz Adeel, et al. Potential mechanisms of abiotic stress tolerance in crop plants induced by thiourea. Front Plant Sci. 2019 Oct 29;10:1336. DOI: 10.3389/fpls.2019.01336

Khaleghi A, Naderi R, Brunetti C, Maserti BE, Salami SA, Babalar M. Morphological, physiochemical and antioxidant responses of Maclura pomifera to drought stress. Sci Rep. 2019 Dec 17;9(1):19250. DOI: 10.1038/s41598-019-55889-y

Pandey P, Irulappan V, Bagavathiannan MV, Senthil-Kumar M. Impact of combined abiotic and biotic stresses on plant growth and avenues for crop improvement by exploiting physio-morphological traits. Front Plant Sci. 2017 Apr 18;8:537. doi: 10.3389/fpls.2017.00537

Baillo EH, Kimotho RN, Zhang Z, Xu P. Transcription factors associated with abiotic and biotic stress tolerance and their potential for crops improvement. Genes (Basel). 2019 Sep 30;10(10):771. DOI: 10.3390/genes10100771

Published

2022-06-08

How to Cite

1.
Potrohov A, Ovcharenko O, Sosnovskaya D. Antioxidant activity of petunias with the heterologous ribonuclease ZRNase II gene infected with tobacco mosaic virus. Innov Biosyst Bioeng [Internet]. 2022Jun.8 [cited 2022Jun.28];6(1):40-5. Available from: http://ibb.kpi.ua/article/view/254464

Issue

Section

Articles