Production of Magnetically Controlled Biosorbents Based on Fungi Pleurotus ostreatus


  • Svitlana Gorobets Igor Sikorsky Kyiv Polytechnic Institute, Ukraine
  • Oleh Radionov Igor Sikorsky Kyiv Polytechnic Institute, Ukraine
  • Oleksiy Kovalev KP "UGCG", Ukraine



Biogenic magnetic nanoparticles, Magnetite, Magneto controlled biosorbent, Pleurotus ostreatus, Heavy metals


Background. Biogenic magnetic nanoparticles (BMN) have been found in representatives of all three superkingdoms of living organisms: bacteria, archaea and eukaryotes (including fungi). At the same time, it was established that the mechanism of biomineralization of BMN is unique for all living organisms. The search for sorbents of biological origin has become one of promising ways of addressing the problem of environmental pollution by heavy metals. Heavy metals are elements of transport emissions and many factories in various industries. These metals getting into the human body cause significant disruption of metabolism and vital functions of the body. Many macromycetes are known to be natural sorbents for heavy metal ions. Laboratory tests use a method of filtering spent sorbent through a paper filter, which is long enough and inefficient. Therefore, it is important to find a more effective way of removing biosorbent from the solution. Such a cheap and efficient method is high-speed magnetic gradient separation.

Objective. The aim of the paper is to obtain a magnetically controlled biosorbent based on a fungus Pleurotus ostreatus, to determine the fraction of the magnetically controlled phase of the fungus biomass when added to the substrate of the magnetic fluid, and to investigate the efficiency of extraction of Fe3+ ions by the fungus biomass of the fungus Pleurotus ostreatus.

Methods. The standard method of cultivation of the fungus Pleurotus ostreatus, the method of high-grade magnetic separation and the method of biosorption of ferric ions were used.

Results. The process of biosorption of ferric ions by a Pleurotus ostreatus grown on substrates with the addition of magnetic fluids of different concentrations was carried out. It is shown that the dry biosorbent based on the biomass of the Pleurotus ostreatus has a high sorption capacity with respect to Fe3+ ions since the efficiency of their extraction for 30 minutes of sorption in the samples grown on the substrate with the addition of magnetite is more than 95%. It is proved that when using biomass of ordinary fungus grown on a magnetic fluid, complete saturation occurs 6 times faster (for 5 minutes), compared to 30 min for biosorbent based on biomass of mushroom grown without magnetic fluid.

Conclusions. Addition of magnetite (concentration 0.1 and 1 mg/ml) to the substrate in the cultivation of fungi Pleurotus ostreatus significantly increases the efficiency of the sorbent. When using the fungus biomass of Pleurotus ostreatus without adding magnetite for 5 minutes, the efficiency of extraction of iron ions is 70%, for 30 min – 80%. When used to grow the magnetic fluid at a concentration of 0.1 mg/ml and 1 mg/ml for almost 5 minutes, almost complete removal of heavy metal ions occurs.


Blakemore R. Magnetotactic bacteria. Science. 1975;190(4212):377-9. DOI: 10.1126/science.170679

Gorobets O, Gorobets S, Koralewski M. Physiological origin of biogenic magnetic nanoparticles in health and disease: from bacteria to humans. Int J Nanomed. 2017;12:4371-95. DOI: 10.2147/ijn.s130565

Vainshtein M, Suzina N, Kudryashova E, Ariskina E. New magnet-sensitive structures in bacterial and archaeal cells. Biol Cell. 2002;94(1):29-35. DOI: 10.1016/s0248-4900(02)01179-6

Kirschvink J. Magnetite biomineralization and geomagnetic sensitivity in higher animals: An update and recommendations for future study. Bioelectromagnetics. 1989;10(3):239-59. DOI: 10.1002/bem.2250100304

Dobson J, Grassi P. Magnetic properties of human hippocampal tissue—Evaluation of artefact and contamination sources. Brain Res Bull. 1996;39(4):255-9. DOI: 10.1016/0361-9230(95)02132-9

Gorobets S, Gorobets O, Bulaievska M, Valverde VM, Hetmanenko K, Sharay I. Biogenic magnetic nanoparticles in representatives of kingdom Fungi. In: Proceedings of IEEE AIM; 2018 Feb 4–7; La Thuile, Italy.

Gorobets S, Gorobets O, Kovalchuk I, Yevzhyk L. Determination of potential producers of biogenic magnetic nano­parti­cles among the fungi representatives of Ascomycota and Basidiomycota divisions. Innov Biosyst Bioeng. 2018;2(4):232-45. DOI: 10.20535/ibb.2018.2.4.147310

Gorobets S, Gorobets O, Magerman A, Gorobets Y, Sharay I. Biogenic magnetic nanoparticles in plants [Internet]. 2020 [cited 2020 March 15]. Available from:

Gorobets O. Biomineralization and synthesis of biogenic magnetic nanoparticles and magnetosensitive inclusions in micro­organisms and fungi. Funct Mater. 2014;21(4):427-36. DOI: 10.15407/fm21.04.427

Markova ME, Uriash VF, Stepanova EA, Gruzdeva AE, Hrishatova NV, Demarin VT, et al. Sorption of heavy metals by higher fungi and chitin of different origin in in vitro experiments. Bulletin of Nizhny Novgorod University. 2008;6:118-24.

Stihi C, Radulescu C, Busuioc G, Popescu IV, Gheboianu A, Ene A. Studies on accumulation of heavy metals from substrate to edible wild mushrooms. Roman J Phys. 2011;56(1-2):257-64.

Abdul-Talib S, Tay C, Abdullah-Suhaimi A, Liew H. Fungal pleurotus ostreatus biosorbent for Cadmium (II) removal in industrial wastewater. J Life Sci Technol. 2013;1(1):65-8. DOI: 10.12720/jolst.1.1.65-68

Wang C, Liu H, Liu Z, Gao Y, Wu B, Xu H. Fe3O4 nanoparticle-coated mushroom source biomaterial for Cr(VI) polluted liquid treatment and mechanism research. R Soc Open Sci. 2018;5(5):171776. DOI: 10.1098/rsos.171776

Ramanaiah S, Venkata Mohan S, Sarma P. Adsorptive removal of fluoride from aqueous phase using waste fungus (Pleurotus ostreatus 1804) biosorbent: Kinetics evaluation. Ecolog Eng. 2007;31(1):47-56. DOI: 10.1016/j.ecoleng.2007.05.006

Iram S, Shabbir R, Zafar H, Javaid M. Biosorption and bioaccumulation of copper and lead by heavy metal-resistant fungal isolates. Arabian J Sci Eng. 2015;40(7):1867-73. DOI: 10.1007/s13369-015-1702-1

Gulich MP, Antomonov MJ, Yemchenko NL, Bisko NA, Yashchenko OV, Ermolenko VP. Sorption of biometals by fungi mycekium from nutrient medium, enriched by their citrares. Microelements in Medicine. 2014;15(2):9-17.

Gorobets S, Mikhailenko N. High-gradient ferromagnetic matrices for purification of wastewaters by the method of magnitoelectrolysis. J Water Chem Technol. 2014;36(4):153-9. DOI: 10.3103/s1063455x14040018

Garibova L. Mashroom Growing. Moscow: Veche; 2005.

Vasiliev AA, Chashchin AN, Lobanova ES, Razinsky MV. Non-stoichiometric magnetite in soils of urbanized territories in Permskii krai. Permskii Agrarniy Vestnik. 2014;(2)6:43-55.

Gorobets S. Method of producing dry magnetically controlled biosorbent. Ukraine patent 118673. 2017.

Gorobets S. Production of magnetically controlled biosorbents based on fungi agaricus bisporus and Lentinula edodes. Biotechnologia Acta. 2019;12(5):63-71. DOI: 10.15407/biotech12.05.063



How to Cite

Gorobets S, Radionov O, Kovalev O. Production of Magnetically Controlled Biosorbents Based on Fungi Pleurotus ostreatus. Innov Biosyst Bioeng [Internet]. 2020Apr.24 [cited 2024Jun.23];4(2):89-94. Available from: