Development of Conditions of Surface Biosynthesis of Prodigiosin Pigment Serratia marcescens

Authors

  • Dmytro Ivanchenko Bogomolets National Medical University, Ukraine

DOI:

https://doi.org/10.20535/ibb.2020.4.1.193513

Keywords:

Prodigiosin, Serratia marcescens, Surface cultivation, Solid nutrient medium

Abstract

Background. Prodigiosin is a red pigment produced as a secondary metabolite by Serratia marcescens, characterized with unique tripyrrole structure and exhibits such pharmacological characteristics as antitumor, antimicrobial, antioxidant, immunodepressant, and also used in various branches of biotechnology as a natural dye or marker of petroleum products. However, pigmentation is only present in a small percentage of isolated cultures among different S. marcescens strain, therefore, the effectiveness of biotechnological productivity is primarily determined by the producer strain, as well as the rational conditions for biosynthesis. Pigment production levels are strongly influenced by various factors, such as the set and ratio of the components of the culture medium (concentration of a particular substrate, C:N ratio) and cultivation conditions (temperature, pH). Besides, the use of relatively low-cost substrates is an important condition for reducing financial costs for the production of culture media and increasing the level of biosynthesis of the target product. Therefore, the determination of the influence of the culture medium for the strains of S. marcescens and the identification of the main factors contributing to the accumulation of the pigment prodigiosin to develop biotechnologies for the production of drugs for therapeutic purposes is relevant.

Objective. The purpose of the paper is investigation of pigment-forming strain of S. marcescens under cultivation conditions on the surface of solid nutrient media, determination of biochemical features, and rational cultivation conditions that contribute to the accumulation of the pigment prodigiosin.

Methods. The strain of S. marcescens isolated from the bentonite clays of Kurtsivskyi deposit (Crimea, Ukraine) was cultured in Petri dishes on solid nutrient media with different temperatures, pH, carbon and nitrogen sources. The influence of various values of the studied factors was established by the level of pigment accumulation, which was determined by the gravimetric method. The acidity of the nutrient medium was determined by the potentiometric method.

Results. The influence of the initial pH values of solid nutrient medium, temperature, and various sources of carbon and nitrogen on the accumulation of pigment during the cultivation of the strain S. marcescens was studied. The temperature, pH, and compounds, sources of carbon and nitrogen, favorable for obtaining the pigment, were determined.

Conclusions. It was found that the rational parameters for cultivation are the temperature +27–28 °C and the acidity of the nutrient medium of 6–7, and the best sources of carbon and nitrogen for prodigiosin accumulation are glycerin and peptone (as well as casein hydrolysate, yeast extract or ammonium (nitrate) inorganic compounds), respectively.

References

Guryanov ID, Karamova NS, Yusupova DV, Gnezdilov OI, Koshkarova LA. Bacterial pigment prodigiosin and its genotoxic effect. Bioorganic Chem. 2013;39:121-28. DOI: 10.1134/s1068162012060040

Wei YH, Chen WC. Enhanced production of prodigiosin-like pigment from Serratia marcescens SMΔR bymedium improvement and oil-supplementation strategies. J Biosci Bioeng. 2005;99:616-22. DOI: 10.1263/jbb.99.616

Wei YH, Yu WJ, Chen WC. Enhanced undecylprodigiosin production from Serratia marcescens SS-1 by medium formulation and amino-acid supplementation. J Biosci Bioeng. 2005;100:466-71. DOI: 10.1263/jbb.100.466

Kalivoda EJ, Stella NA, Aston MA, Fender JE, Thompson PP, Kowalski RP, et al. Cyclic AMP negatively regulates prodigiosin production by Serratia marcescens. Res Microbiol. 2010;161:158-67. DOI: 10.1016/j.resmic.2009.12.004

Giri A, Anandkumar N, Muthukumaran G, Pennathur G. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol. 2004;4:1-10. DOI: 10.1186/1471-2180-4-11

Chang CC, Chen WC, Ho SF, Wu HS, Wei YH. Development of natural anti-tumor drugs by microorganisms. J Biosci Bioeng. 2011;111:501-11. DOI: 10.1016/j.jbiosc.2010.12.026

Yamazaki G, Nishimura S, Ishida A, Kanagasabhapathy M, Zhou X, Nagata S, et al. Effect of salt stress on pigment production of Serratia rubidaea N-1: a potential indicator strain for screening quorum sensing inhibitors from marine microbes. J Gen Appl Microbiol. 2006;52:113-7. DOI: 10.2323/jgam.52.113

Su WT, Tsou TY, Liu H.L. Response surface optimization of microbial prodigiosin production from Serratia marcescens. J Taiwan Inst Chem Eng. 2011;42:217-22. DOI: 10.1016/j.jtice.2010.05.009

Chen WC, Yu WJ, Chang CC, Chang JS, Huang SH, Chang CH, et al. Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy. Biochem Eng J. 2013;78:93-100. DOI: 10.1016/j.bej.2013.02.001

de Araújo HWC, Fukushima K, Takaki GMC. Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules. 2010;15:6931-40. DOI: 10.3390/molecules15106931

Stankovic N, Radulovic V, Petkovic M, Vuckovic I, Jadranin M, Vasiljevic B, et al. Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. Appl Microbiol Biotechnol. 2012;96:1217-31. DOI: 10.1007/s00253-012-4237-3

Mo SJ, Kim JH, Oh CH. Different effects of acidic pH shock on the prodiginine production in Streptomyces coelicolor M511 and SJM1 mutant. J Microbiol Biotechnol. 2013;23:1454-9. DOI: 10.4014/jmb.1307.07067

Bergey DH, Holt JG. Bergey's Manual of Determinative Bacteriology. 9th ed. Baltimore: Williams & Wilkins, 1993.

Phatake YB, Dharmadhikari SM. Isolation and screening of prodigiosin production bacteria and characterization of produced pigment. Int J Sci Nature. 2016;7(1):202-9.

Darshan N, Manonmani HK. Prodigiosin and its potential applications. J Food Sci Technol. 2015;52(9):5393-407. DOI: 10.1007/s13197-015-1740-4

Vadzinsky RN. Statistical calculations in the Excel environment. User library. St. Petersburg: Piter Publ.; 2016. 608 p.

Colquhoun D. The reproducibility of research and the misinterpretation of p-values. R Soc Open Sci. 2017;4(12):171085. DOI: 10.1098/rsos.171085

Published

2020-03-02

How to Cite

1.
Ivanchenko D. Development of Conditions of Surface Biosynthesis of Prodigiosin Pigment Serratia marcescens. Innov Biosyst Bioeng [Internet]. 2020Mar.2 [cited 2021Sep.22];4(1):19-25. Available from: http://ibb.kpi.ua/article/view/193513

Issue

Section

Articles