Suspension Culture of Erythrocytes in the Assessment of the Detergent Functional Component Toxicity Level
Keywords:Denaturation of hemoglobin, Hemolysis of erythrocytes, Anionic surfactants, Amphoteric surfactants, Nonionic surfactants, Toxicity, Damaging action
Background. The study is devoted to the assessment of the cytotoxic properties of various surfactants by a method that is highly sensitive to identification of their negative effect on living mammalian cells.
Objective. The aim of the work is to carry out a comparative assessment of modern surfactants in terms of the degree of manifestation of cytotoxic properties.
Methods. The study of 10 surfactants was carried out with a new method for assessing the cytotoxic effect of agents based on surfactants, which is a modification of The RBC Hemolysis Test and Hemoglobin Denaturation Test. This method calculates the integral cytotoxicity index, which takes into account the data obtained by two tests. The following indicators were determined in the work: the percentage of hemoglobin denaturation in a 1% surfactant solution (D, %), the concentration of the surfactant solution at which hemolysis of 50% erythrocytes occurs (H50,%), and the cytotoxicity coefficient (Cc, conv. un.) of surfactants which is calculated by the formula Cc = H50/D×10000.
Results. According to the integral Cc indicator, the studied surfactants can be ranked according to the degree of toxicity reduction in the following order: sodium lauryl sulfate (0.09) – sodium laurate sulfate (0.13) – cocamidopropyl betaine (0.27) – sodium salt of polyethoxysulfosuccinate (0.27) – alkyldimethyl betaine (1.07) – disodium cocoamphodiacetate (1.99) – sodium salt n-palmethylglutamic acid (3.22) – cocoglucoside (27.86). Diethanolamides of coconut oil fatty acids and polyquaternium 7 in the studied concentration range (up to 1%) did not show denaturation properties.Conclusions. Studies have shown that surfactants significantly differ from each other by the level of the damaging effect, the most aggressive components were anionic and amphoteric surfactants, nonionic surfactants have a significantly lower cytotoxic effect (10 times). The results obtained should be taken into account when creating soft agents based on surfactants.
Khamidulina KhKh. Main trends of the international activities in the sphere of chemical safety and their implementation in the Russian Federation. Toxicol Rev. 2011;6:2-7.
Timofeiev G. Safety and efficacy on the way of cosmetic products to the costumers. SORF J. 2007;5:34-40.
Ernandes YI, editor. New cosmetology. Vol І. Moscow: Klavel Ltd.; 2005. 424 p.
Ernandes YI, Margolina A, Petruhina A. Lipid barrier of skin and cosmetics. Moscow: Klavel Ltd.; 2003. 340 p.
Corazza M, Laurla MM, Zappaterra M, Bianchi A, Virgili A. Surfactants, skin cleansing protagonists. J Eur Acad Dermatol Venerol. 2010;24(1):1-6. DOI: 10.1111/j.1468-3083.2009.03349.X
Voloschenko OI, Raietska OV, Yalovenko OI, Kyzmina AI. Method for the estimation of cytotoxic effect of the agents on the basis of surfactants. of Ukraine Patent 28727. 2007 Dec 25.
Voloschenko OI, Raietska OV, Yalovenko OI. The current state of the alternative modeling of the assessment of skin irritation (review). Environment and Health. 2008; 3:67-71.
Hatao M, Murakami N, Sakamoto K, Ohnuma M, Matsushige C, Kakishima H, et al. Interlaboratory Validation of the in vitro Eye IrritationTests for Cosmetic Ingredients. (4) Hemoglobin Denaturation Test. Toxicol in vitro. 1999;13(1):125-37. DOI: 10.1016/S0887-2333(98)00067-8
Antomonov M. Mathematical processing and analysis of biomedical data. 2nd ed. Kyiv: Medinform; 2018. 579 p.
Pletniov MJ. Surfactants and compositions. Moscow: Klavel Ltd.; 2002. 768 p.
How to Cite
Copyright (c) 2020 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.IBB articles are published under Creative Commons licence:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.