Obtaining Mycelial Biomass of Medicinal Fungi Grifola frondosa and Laetiporus sulphureus on Synthetic Media





Biotechnology, Grifola frondosa, Laetiporus sulphureus, Xylotrophic basidium fungi, Submerged cultivation, Synthetic liquid nutrient medium, Mycelial biomass


Background. The negative impact of anthropogenic factors on the environment and the wrong way of life of a significant part of the population leads to the need to search and develop new food products and therapeutic and preventive medicines or to improve existing technologies for their production, based on different biological objects, including xylotrophic basidium fungi. Therefore, definition of condition influence of submerged cultivation for strains Grifola frondosa and Laetiporus sulphureus and definition of principal factors which favour the accumulation of mycelial biomass to work out home biotechnologies of receiving food, medical preventive, and cosmetic preparations is relevant.

Objective. The purpose of the paper is analysis of strains G. frondosa and L. sulphureus under condition of submerged cultivation on synthetic medium, definition of physiological-biochemical peculiarities of these fungi and determination of liquid medium parameters, promoting the accumulation of biomass.

Methods. Submerged cultivation of strains 1707 Grifola frondosa (Dicks: Fr.) S.F. Gray and 1518 Laetiporus sulphureus (Bull.: Fr.) Murrill was carried out in Erlenmeyer flaks on an orbital rocking-chair on liquid synthetic nutrient medium with different pH values, nitrogen and carbon sources, under conditions of constant stirring at temperature of +28 °С. The influence of different values of the studied factors was defined by the level of accumulation of mycelial biomass determined by the weight method. The acidity of the nutrient and culture fluid was determined by potentiometric method.

Results. The influence of the initial pH value of a liquid synthetic nutrient medium and various sources of carbon and nitrogen on the biomass accumulation during the cultivation of basidium fungi G. frondosa and L. sulphureus was studied. pH values, as well as sources of carbon and nitrogen favourable for obtaining mycelial biomass were defined.

Conclusions. The growth of two species of medicinal xylotrophic basidium fungi Grifola frondosa 1707 and Laetiporus sulphureus 1518 on synthetic medium under condition of submerged cultivation at different initial pH values and with different sources of carbon and nitrogen was studied. The pH value favourable for the growth of submerged mycelium was determined and it was established that the best sources for the accumulation of mycelial biomass for G. frondosa 1707 strain are carbon ones – starch and glucose, and nitrogen source – peptone and ammonium nitraten. For L. sulphureus 1518, the favorable carbon source is starch, a source of nitrogen is peptone. For the submerged cultivation of two types of xylotrophic medicinal basidium fungi, the use of a synthetic medium has been proposed: for G. frondosa 1707 – with an initial pH 6.9, glucose and ammonium nitrate, and for L. sulphureus 1518 – with an initial pH 6.6, starch and peptone.


Buchalo AS, Babitskaya BG, Bisko NA, Wasser SP, Dudka IA, Mytropolska NY, et al. Biological features of medicinal macromycetes in culture. Kyiv: Alterpres; 2011. Volume 1; 212 p.

Bisko NA, Babitskaya VL, Buchalo AS, Krupodorova TA, Lomberg ML, Mykchaylova OB, et al. Biological features of medicinal macromycetes in culture. Kyiv: Alterpress; 2012. Volume 2; 459 p.

Panchak LV. Russulaceae family mushrooms lectins: function, purification, structural features and possibilities of practical applications. Biotechnologia Acta. 2019;12(1):29-38 DOI: 10.15407/biotech12.01.029

Poyedinok NL, Tugay TI, Tugay AV, Mykchaylova OB, Sergiichuk NN, Negriyko AM. Influence of nitrogen concentration on photoinduced growth, enzymatic activity and melanine synthesis by Inonotus obliquus (Ach.:Pers.) Pilát. Biotechnologia Acta. 2019;12(4):34-41. DOI: 10.15407/biotech12.04.034

Vetter J. Biological values of cultivated mushrooms – A review. Acta Alimentaria. 2019;48(2):229-40. DOI: 10.1556/066.2019.48.2.11

Zhang L, editor. Progress in molecular biology and translational science: Glycans and glycosaminoglycans as clinical bio­markers and therapeutics. Academic Press; 2019. Volume 163; 551 p. DOI: 10.1016/s1877-1173(19)30072-9

Zhang ZQ, Liu LP, Lei L, Wang CN, Tang QL, Wu TX. Antioxidative and immunomodulatory activities of the exopolysaccharides from submerged culture of hen of the woods or Maitake culinary-medicinal mushroom, Grifola frondosa (agaricomycetes) by addition of Rhizoma Gastrodiae extract and its main components. Int J Med Mushrooms. 2019;21(8):825-39. DOI: 10.1615/IntJMedMushrooms.2019031597

Grundemann C, Reinhardt JK, Lindequist U. European medicinal mushrooms: Do they have potential for modern medicine? Phytomedicine. 2020;66:153131. DOI: 10.1016/j.phymed.2019.153131

Younis AM, Yosri M, Stewart JK, In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus. Ann Agricult Sci. 2019;64:79-87. DOI: 10.1016/j.aoas.2019.05.001

Acharya K, Ghosh S, Khatua S, Mitra P. Pharmacognostic standardization and antioxidant capacity of an edible mushroom Laetiporus sulphureus. J Consumer Protect Food Safety. 2016;1(1):33-42. DOI: 10.1007/s00003-015-0977-1

Hwang HS, Yun JW. Hypoglycemic effect of polysaccharides produced by submerged mycelial culture of Laetiporus sulphureus on streptozotocin-induced diabetic rats. Biotechnol Bioproc Eng. 2010;15:173-81. DOI: 10.1007/s12257-009-0160-6

Koch J, Witt S, Lindequist U. The influence of selected higher basidiomycetes on the binding of lipopolysaccharide to CD14+ cells and on the release of cytokines. Int J Med Mushrooms. 2002;4:229-35. DOI: 10.1615/IntJMedMushr.v4.i3.50

Lindequist U, Niedermeyer THJ, Jülich WD. The Pharmacological potential of mushrooms. eCAM. 2005;2(3):285-99. DOI: 10.1093/ecam/neh107

Khatua S, Ghosh S, Acharya K. Laetiporus sulphureus (Bull.: Fr.) Murr. as food as medicine. Pharmacog J. 2017;9(6):1-15. DOI: 10.5530/pj.2017.6s.151

Younis AM, Yosri M, Stewart JK. In vitro evaluation of pleiotropic properties of wild mushroom Laetiporus sulphureus. Ann Agricult Sci. 2019,64:79-87. DOI: 10.1016/j.aoas.2019.05.001

Dzygun LP. Medicinal xylotrophic basidiomycetes Laetiporus sulphureus (Bull.: Fr.) Murill – Perspective object of biotechnology. Naukovi Visti NTUU KPI. 2011;3:40-9.

Didukh JP, editor. Red data book of Ukraine. Plant kingdom. Kyiv: Hlobalkonsaltyng; 2009. 900 p.

Bisko NA, Lomberg ML, Mytropolska NY, Mykchaylova OB. The IBK Mushroom Culture Collection. Kyiv: Alterpres; 2016. 120 p.

Buchalo AS. Higher edible Basidiomycetes in pure culture. Kyiv: Naukova Dumka; 1988. 144 p.

Lee BC, Bae JT, Pyo HB, Choe TB, Kim SW, Hwang HJ, et al. Biological activities of the polysaccharides produced from submerged culture of the edible Basidiomycete Grifola frondosa. Enzyme Microb Technol. 2003;32(5):574-81. DOI: 10.1016/s0141-0229(03)00026-7



How to Cite

Dzyhun L, Linovytska V. Obtaining Mycelial Biomass of Medicinal Fungi Grifola frondosa and Laetiporus sulphureus on Synthetic Media. Innov Biosyst Bioeng [Internet]. 2019Dec.20 [cited 2023Jun.9];3(4):239-45. Available from: http://ibb.kpi.ua/article/view/186329