Obtaining Mycelial Biomass of Medicinal Fungi Grifola frondosa and Laetiporus sulphureus on Synthetic Media
DOI:
https://doi.org/10.20535/ibb.2019.3.4.186329Keywords:
Biotechnology, Grifola frondosa, Laetiporus sulphureus, Xylotrophic basidium fungi, Submerged cultivation, Synthetic liquid nutrient medium, Mycelial biomassAbstract
Background. The negative impact of anthropogenic factors on the environment and the wrong way of life of a significant part of the population leads to the need to search and develop new food products and therapeutic and preventive medicines or to improve existing technologies for their production, based on different biological objects, including xylotrophic basidium fungi. Therefore, definition of condition influence of submerged cultivation for strains Grifola frondosa and Laetiporus sulphureus and definition of principal factors which favour the accumulation of mycelial biomass to work out home biotechnologies of receiving food, medical preventive, and cosmetic preparations is relevant.
Objective. The purpose of the paper is analysis of strains G. frondosa and L. sulphureus under condition of submerged cultivation on synthetic medium, definition of physiological-biochemical peculiarities of these fungi and determination of liquid medium parameters, promoting the accumulation of biomass.
Methods. Submerged cultivation of strains 1707 Grifola frondosa (Dicks: Fr.) S.F. Gray and 1518 Laetiporus sulphureus (Bull.: Fr.) Murrill was carried out in Erlenmeyer flaks on an orbital rocking-chair on liquid synthetic nutrient medium with different pH values, nitrogen and carbon sources, under conditions of constant stirring at temperature of +28 °С. The influence of different values of the studied factors was defined by the level of accumulation of mycelial biomass determined by the weight method. The acidity of the nutrient and culture fluid was determined by potentiometric method.
Results. The influence of the initial pH value of a liquid synthetic nutrient medium and various sources of carbon and nitrogen on the biomass accumulation during the cultivation of basidium fungi G. frondosa and L. sulphureus was studied. pH values, as well as sources of carbon and nitrogen favourable for obtaining mycelial biomass were defined.
Conclusions. The growth of two species of medicinal xylotrophic basidium fungi Grifola frondosa 1707 and Laetiporus sulphureus 1518 on synthetic medium under condition of submerged cultivation at different initial pH values and with different sources of carbon and nitrogen was studied. The pH value favourable for the growth of submerged mycelium was determined and it was established that the best sources for the accumulation of mycelial biomass for G. frondosa 1707 strain are carbon ones – starch and glucose, and nitrogen source – peptone and ammonium nitraten. For L. sulphureus 1518, the favorable carbon source is starch, a source of nitrogen is peptone. For the submerged cultivation of two types of xylotrophic medicinal basidium fungi, the use of a synthetic medium has been proposed: for G. frondosa 1707 – with an initial pH 6.9, glucose and ammonium nitrate, and for L. sulphureus 1518 – with an initial pH 6.6, starch and peptone.
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