Vulnerabilities in the Production of Biogas from the Fat-Containing Tannery Waste
Background. The problem of disposal of the fat-containing tannery waste can be solved by anaerobic fermentation. This raises the important question of the effect of inhibitor compounds on the process of methanogenesis and on the output of biogas. In particular, the effects of NaCl and NaHCO3 used in technology and antibiotics that accumulate in subcutaneous fat when used in animal husbandry for digestion of fat-containing raw materials have not yet been studied.
Objective. The purpose of the paper is determination of possible influence of salts and antibiotics on the process of methanogenesis and on the output of biogas and methane.
Methods. The study was conducted in laboratory reactors with a volume of 0.5 dm3 under anaerobic conditions at 38 ± 1 °С with the addition of different concentrations of salts and antibiotics (the sample was used without the addition of test compounds to control the process). The anaerobic association of microorganisms in the form of an inoculum served as the biological agent.
Results. The effect of salt content on the destabilization of methanogenesis during digestion of fat-containing raw materials and the effect of the antibiotic content of tetracycline and norfloxacin on the yield of biogas from fatty raw materials were determined. Thus, with the use of NaCO3, the greatest inhibition occurs at a concentration of 11.4 g/dm3 (decrease in biogas output by 54%), with a further increase in the concentration to 22.8 g/dm3 an increase of 18.2% is observed compared to the concentration of 11.4 g/dm3. It is shown that the concentration of methane in biogas with the use of NaCO3 decreases with increasing concentration of the inhibitor, which confirms its negative impact on the process of methanogenesis.Conclusions. Biogas yield is increased by 17% at a NaCl concentration of 3.8 g/dm3, and biogas and methane production decreases even more at NaCl concentrations of 7.6 g/dm3. When using antibiotics, tetracycline and norfloxacin, the allowable rate in raw materials is 2.5 mg/dm3 for both antibiotics.
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Golub N, Shynkarchuk M, Kozlovets O. Obtaining biogas during fermentation of fat-containing wastes of leather production. Eastern-European Journal of Enterprise Technologies. 2017;10(90):4-10. DOI: 10.15587/1729-4061.2017.114216
Golub N, Shinkarchuk M, Kozlovets O. The way of increasing biogas at thefermenting fat-containing waste tannery. Herald оf Khmelnytskyi National University. 2018;2:103-7.
Zhang S, Gu J, Wang C, Wang P, Jiao S, Li He Z, et al. Characterization of antibiotics and antibiotic resistance genes on an ecological farm system. J Chem. 2015;2015:526143. DOI: 10.1155/2015/526143
Yenigün O, Demirel B. Ammonia inhibition in anaerobic digestion: a review. Proc Biochem. 2013;48(5-6):901-11. DOI: 10.1016/j.procbio.2013.04.012
Chen Y, Cheng JJ, Creamer KS. Inhibition of anaerobic digestion process: a review. Biores Technol. 2008;99(10):4044-64. DOI: 10.1016/j.biortech.2007.01.057
Mudhoo A, Kumar S. Effects of heavy metals as stress factors on anaerobic digestion processes and biogas production from biomass. Int J Environ Sci Technol. 2013;10:1383-98. DOI: 10.1007/s13762-012-0167-y
Nikovskaya GN, Kalinichenko KV. Biotechnology of utilization of municipal wastewater sediments. Biotechnologia Acta. 2014;3(7):21-32. DOI: 10.15407/biotech7.03.021
Palmeiro T, Val Del Río A, Mosquera-Corral A, Campos JL, Méndez R. Effect of salts on the anaerobic digestion of aerobic granular sludge [Internet]. Redbiogas.cl. 2013 [cited 2019 Nov 20]. Available from: http://www.redbiogas.cl/wp-content/uploads/2013/07/IWA-10900.pdf
Oh G, Zhang L, Jahng D. Osmoprotectants enhance methane production from the anaerobic digestion of food wastes containing a high content of salt. J Chem Technol Biotechnol. 2019;83(9):1204-10. DOI: 10.1002/jctb.1923
Turcio AE, Weichgrebe D, Papenbroc J. Effect of salt and sodium concentration on the anaerobic methanisation of the halophyte Tripolium pannonicum. Biomass and Bioenergy. 2016;87:69-77. DOI: 10.1016/j.biombioe.2016.01.013
Anwar N, Wang W, Zhang J, Li Y, Chen C, Liu G, et al. Effect of sodium salt on anaerobic digestion of kitchen waste. Water Sci Technol. 2016;73(8):1865-71. DOI: 10.2166/wst.2016.035
Lefebvre O, Quentin S, Torrijos M, Godon JJ, Delgenès JP, Moletta R. Impact of increasing NaCl concentrations on the performance and community composition of two anaerobic reactors. Appl Microbiol Biotechnol. 2006;75(1):61-9. DOI: 10.1007/s00253-006-0799-2
Kasali GB, Senior E, Watson-Craik I A. Sodium bicarbonate effects on the anaerobic digestion of refuse. J Chem Technol Biotechnol. 2007;45(4):279-89. DOI: 10.1002/jctb.280450405
Hierholtzer A, Akunna JC. Modelling sodium inhibition on the anaerobic digestion process. Water Sci and Technol. 2012;66(7):1665-73. DOI: 10.2166/wst.2012.345
Wang S, Hou X, Su H. Exploration of the relationship between biogas production and microbial community under high salinity conditions. Sci Rep. 2017;7:1-10. DOI: 10.1038/s41598-017-01298-y
Cetecioglu Z, Ince B, Gros M, Rodriguez-Mozaz S. Chronic impact of tetracycline on the biodegradation of an organic substrate mixture under anaerobic conditions. Water Res. 2013;47(9):2959-69. DOI: 10.1016/j.watres.2013.02.053
Yang Y, Huang W, Huang W. Antibiotic inhibition on anaerobic digestion of animal manure and controlling strategies: a short review. Clean Soil Air Water. 2019;1(47):1-10. DOI: 10.1002/clen.201700653
Golub N, Shynkarchuk M, Kozlovets O, Potapova M, inventors. Installation for the production of biogas from fat-containing waste. Ukraine patent 135483. 2019 July 10.
Standards USSR. Organic fertilizers. General requirements for the method of analysis. Moscow; 1986. GOST 26712-94.
Leibniz E, Struppe HG. Guide to gas chromatography. Part 1. Moscow: Mir; 1988. 480 p.
Shimadzu High–Performance Liquid Chromatograph [Internet]. Shimadzu.eu. 2010 [cited 2019 Nov 20]. Available from: https://www.shimadzu.eu/sites/shimadzu.seg/files/application_handbook_hplc.pdf
Seadi T, Rutz D, Prassl H, Kеttner M, Finsterwalder T, Volk S, et al. Biogas handbook. University of Southern Denmark Esbjerg; 2008. 126 p.
Franke-Whittle IH, Walter A, Ebner C, Insam H. Investigation into the effect of high concentrations of volatile fatty acids in anaerobic digestion on methanogenic communities. Waste Management. 2014;1(32):2080-9. DOI: 10.1016/j.wasman.2014.07.020
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