Potential of Lemnoideae Species for Phytoremediation of Fresh Water with Elevated Manganese Concentration
Background. Wastewater treatment using physical, chemical, and biological methods is primary solution for the reduction of water pollution that reaching the critical thresholds. The members of subfamily Lemnoideae, commonly called duckweed, are considered the most efficient aquatic plants for wastewater remediation. Although properties of duckweed to survive in water with high concentration of heavy metal ions such as chromium, cobalt, lead, nickel and cuprum are well documented, the growth of duckweed in water with high concentrations of manganese and the efficiency of retention of manganese from water by these species has not been estimated.
Objective. Four duckweed species (Spirodela polyrhiza, Landoltia punctata, Lemna aequinoctialis, and L. turionifera) were used for establishment of influence Mn on their vitality and growth and for studying their potential for phytoremediation of fresh water with elevated manganese concentration.
Methods. Duckweed collected in Eastern China was introduced in tissue culture in vitro by surface sterilization. The identification of the collected duckweed species was determined by DNA barcoding using primers specific for chloroplast intergenic spacers atpF-atpH (ATP) and psbK-psbL (PSB). The experiments for establishment of influence Mn on duckweed growth carried out in aseptic condition. To determinate concentration of Mn, the samples of different water type (Hongze Lake, ponds around Hongze Lake, Huaian local municipal sewage plant and industrial sewage plant) were analyzed by the Inductively Coupled Plasma Optical Emission Spectrometry.
Results. The most sensitive duckweed to Mn was S. polyzhiza, the first characteristic symptoms of toxicity like brown spots have appeared when concentration of Mn was 40 mg/L, the concentration 200 mg/L Mn resulted in chlorosis and death of fronds. L. aequinoctialis and L. turionifera had similar effects in SH medium supplemented with 650 mg/L and 975 mg/L Mn, respectively. L. punctata was the most tolerant duckweed to Mn plants continued to grow even at concentration 975 mg/L. Response of duckweed on Mn was dependent on availability of nitrogen in nutrient medium. Using four duckweed species for treatment of water containing 4.12 mg/L Mn allowed to reduce concentration until safe level of standard (0.1 mg/L Mn).Conclusions. All investigated duckweed species (S. polyrhiza, L. punctata, L. aequinoctialis, and L. turionifera) were characterized by a high level of resistance to manganese, especially L. punctata. Response of duckweed on Mn was dependent on availability of nitrogen in nutrient medium. The tested species of subfamily Lemnoideae were high effective for phytoremediation of water with elevated manganese concentration.
Graedel TE. Inorganic elements, hydrides, oxides, and carbonates. In: Chemical compounds in the atmosphere. New York: Academic Press; 1978. 452 p.
Williams M, Todd GD, Roney N, Crawford J, Coles C, McClure PR, et al. Toxicological profile for manganese. Atlanta: Agency for Toxic Substances and Disease Registry (US); 2012.
Moore JW. Inorganic contaminants of surface water: research and monitoring priorities. New York: Springer-Verlag; 1991. 334 p. DOI: 10.1007/978-1-4612-3004-5
Jaques AP. National inventory of sources and emissions of manganese (1984), Report EPS 5/MM/1/87. Ottawa: Minstry of Supply and Services Canada; 1987. 49 p.
McNeely RN, Neimanis VP, Dwyer L. Water quality sourcebook, a guide to water quality parameters. Ottawa: Environment Canada, Inland Waters Directorate, Water Quality Branch; 1979. 88 p.
Drinking water health advisory for manganese. Washington: U.S. Environmental Protection Agency Office of Water, Health and Ecological Criteria Division; 2004.
Health effects support document for manganese. Washington: U.S. Environmental Protection Agency Office of Water, Health and Ecological Criteria Division; 2003.
Zhang Q, Pan E, Liu L, Hu W, He Y, Xu Q, et al. Study on the Relationship between manganese concentrations in rural drinking water and incidence and mortality caused by cancer in Huai’an City. BioMed Res Int. 2014;2014:645056. DOI: 10.1155/2014/645056
Nutrient requirements of dairy cattleю 7th ed. Washington: National Research Council, National Academy Press; 2001.
Borisjuk N, Chu P, Gutierrez R, Zhang H, Acosta K, Friesen N, et al. Assessment, validation and deployment strategy of a two-barcode protocol for facile genotyping of duckweed species. Plant Biol (Stuttg). 2015 Jan;17 Suppl 1:42-9. DOI: 10.1111/plb.12229
Zhou Y, Chen G, Peterson A, Zha X, Cheng J, Li S, et al. Biodiversity of duckweeds in Eastern China and their potential for bioremediation of municipal and industrial wastewater. J Geosci Environ Protec. 2018 Apr 8;6:108-16. DOI: 10.4236/gep.2018.63010
Ziegler P, Sree KS, Appenroth KJ. Duckweeds for water remediation and toxicity testing. Toxicol Environ Chem. 2016 Nov 25;98(10):1127-54. DOI: 10.1080/02772248.2015.1094701
van der Steen NP, Nakiboneka P, Mangalika L, Ferrer AVM, Gijzen HJ. Effect of duckweed cover on greenhouse gas emissions and odour release from waste stabilisation ponds. Water Sci Technol. 2003;48(2):341-8. DOI: 10.2166/wst.2003.0139
Mohedano RA, Tonon G, Costa RHR, Pelissari C, Belli Filho P. Does duckweed ponds used for wastewater treatment emit or sequester greenhouse gases? Sci Total Environ. 2019 Nov 15;691:1043-50. DOI: 10.1016/j.scitotenv.2019.07.169
Banu Doğanlar Z. Metal accumulation and physiological responses induced by copper and cadmium in Lemna gibba, L. minor and Spirodela polyrhiza. Chemical Speciation & Bioavailability. 2013 Jan 1;25(2):79-88. DOI: 10.3184/095422913X13706128469701
Bokhari SH, Ahmad I, Mahmood-Ul-Hassan M, Mohammad A. Phytoremediation potential of Lemna minor L. for heavy metals. Int J Phytoremed. 2016 Jan 2;18(1):25-32. DOI: 10.1080/15226514.2015.1058331
Chaudhuri D, Majumder A, Misra AK, Bandyopadhyay K. Cadmium removal by Lemna minor and Spirodela polyrhiza. Int J Phytoremed. 2014 Nov 2;16(11):1119-32. DOI: 10.1080/15226514.2013.821446
Upatham ES, Boonyapookana B, Kruatrachue M, Pokethitiyook P, Parkpoomkamol K. Biosorption of cadmium and chromium in duckweed Wolffia globosa. Int J Phytoremed. 2002 Apr 1;4(2):73-86. DOI: 10.1080/15226510208500074
Uysal Y, Taner F. Effect of pH, temperature, and lead concentration on the bioremoval of lead from water using Lemna minor. Int J Phytoremed. 2009 Sep 11;11(7):591-608. DOI: 10.1080/15226510902717648
Schenk RU, Hildebrandt AC. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot. 1972 Jan 1;50(1):199-204. DOI: 10.1139/b72-026
Kumaravel S, Alagusundaram K. Determination of mineral content in Indian spices by ICP-OES. Oriental J Chem. 2014 Jul 1;30:631-6. DOI: 10.13005/ojc/300231
Schmidt SB, Jensen PE, Husted S. Manganese deficiency in plants: the impact on photosystem II. Trends Plant Sci. 2016 Jul;21(7):622-32. DOI: 10.1016/j.tplants.2016.03.001
Wissemeier AH, Horst WJ. Effect of light intensity on manganese toxicity symptoms and callose formation in cowpea (Vigna unguiculata (L.) Walp.). Plant Soil. 1992 Jun 1;143(2):299-309. DOI: 10.1007/BF00007886
Foy CD, Scott BJ, Fisher JA. Genetic differences in plant tolerance to manganese toxicity. In: Manganese in soils and plants. Kluwer Academic Publishers; 1988. Volume 33, Developments in plant and soil sciences; p. 293-307. DOI: 10.1007/978-94-009-2817-6_20
GOST Style Citations
Copyright (c) 2019 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.