DOI: https://doi.org/10.20535/ibb.2019.3.3.174795

The Effect of the Mineral and Carbohydrate Composition of the Nutrient Medium on the Efficiency of Microclonal Propagation of Origanum Vulgare L. In Vitro

Anastasiia Fokina, Tatiana Satarova, Kateryna Derkach

Abstract


Background. Oregano (Origanum vulgare L.) is an essential oil plant whose biologically active substances possess antimicrobial, antiviral, antitumor, wound healing properties. The genetic diversity of this medicinal plant requires a detailed study of the sensitivity of its genotypes in vitro culture to ensure a high level of microclonal propagation efficiency.

Objective. The aim of the paper is to develop elements of the technology of microclonal propagation of oregano by activating buds growing in vitro, in particular, to study the effect of the mineral, vitamin, and carbohydrate composition of the nutrient medium on shoots multiplication in different cultivars and to compare the morphogenetic potential of the apical and lateral buds in an in vitro culture.

Methods. The method of activation of the buds growing (method of sterile cutting) is applied. The variants of nutrient medium with full and halved content of macro-, microsalts, and vitamins of MS medium, as well as medium that differed in carbohydrate composition and contained sucrose (15 or 30 g/l) or glucose (15 or 30 g/l) were used. Such indicators as the number of newly formed shoots per one cutting and the reproduction rate were recorded and analyzed.

Results. The optimal medium for most genotypes for new shoots from both apical and lateral buds in vitro is MS+30 g/l glucose. On average, the specific number of shoots obtained from cuttings of lateral buds was 1.82 times higher than the specific number of shoots obtained from cuttings of apical buds. The sample Д10 was found to be the most productive, for which on MS + 30 g/l glucose medium the highest number of newly formed shoots was obtained (2.9 pieces per a cutting for apical buds and 3.7 pieces per a cutting for lateral ones). The coefficient of multiplication for genotype Д10 on MS + 30 g/l glucose was 14.6 and 19.3 internodes per a cutting, respectively, for apical and lateral buds. On average, the coefficient of multiplication for cuttings of lateral buds was 1.47 times higher than that one for cuttings of apical buds.

Conclusions. The effect of the genotype and composition of the nutrient medium on the efficiency of microclonal propagation by activating apical and lateral buds in the sterile cutting of oregano was shown. Among the studied variants of the carbohydrate and mineral compositions of the medium, the most effective was MS+30 g/l glucose. Genotype Д10 revealed the greatest potential for microclonal reproduction among the five samples.

Keywords


Oregano Origanum vulgare L.; Microclonal propagation in vitro; Reproduction rate; Buds activation; Cuttings

References


Ding H, Chou T, Liang C. Antioxidant and antimelanogenic properties of rosmarinic acid methyl ester from Origanum vulgare. Food Chem. 2010;123(2):254-62. DOI: 10.1016/j.foodchem.2010.04.025

Han X, Parker T. Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano (Origanum vulgare) essential oil in a human skin disease model. Biochimie Open. 2017;4:73-7. DOI: 10.1016/j.biopen.2017.02.005

Zhang X, Guo Y, Wang C, Li G, Xu J, Chung H, et al. Phenolic compounds from Origanum vulgare and their antioxidant and antiviral activities. Food Chem. 2014;152:300-6. DOI: 10.1016/j.foodchem.2013.11.153

Mastro G, Tarraf W, Verdini L, Brunetti G, Ruta C. Essential oil diversity of Origanum vulgare L. populations from Southern Italy. Food Chem. 2017;235:1-6. DOI: 10.1016/j.foodchem.2017.05.019

Morshedloo M, Salami S, Nazeri V, Maggi F, Craker L. Essential oil profile of oregano (Origanum vulgare L.) populations grown under similar soil and climate conditions. Ind Crops Prod. 2018;119:183-90. DOI: 10.1016/J.INDCROP.2018.03.049

State register of plant varieties suitable for dissemination in Ukraine in 2019 | Ukrainian Institute for Plant Variety Examination [Internet]. Sops.gov.ua. 2019 [cited 25 July 2019]. Available from: https://sops.gov.ua/reestr-sortiv-roslin

Chun S, Vattem D, Lin Y, Shetty K. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochem. 2005;40(2):809-16. DOI: 10.1016/j.procbio.2004.02.018

Habibi P, de Sa M, da Silva A, Makhzoum A, da Luz Costa J, Borghetti I, et al. Efficient genetic transformation and rege­neration system from hairy root of Origanum vulgare. Physiol Mol Biol Plants. 2016;22(2):271-7. DOI: 10.1007/s12298-016-0354-2

Pandey A, Belwal T, Tamta S, Bhatt I, Rawal R. Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L. Mol Biol Rep. 2019;46(2):2231-41. DOI: 10.1007/s11033-019-04678-x

Fokina A, Satarova T, Smetanin V, Kucenko N. Optimization of microclonal propagation in vitro of Oregano (Origanum vulgare). Biosyst Divers. 2018;26(2):98-102. DOI: 10.15421/011815

Shetty K, Curtis O, Levin R, Witkowsky R, Ang W. Prevention of vitrification associated with in vitro shoot culture of oregano. (Origanum vulgare) by Pseudomonas spp. J Plant Physiol. 1995;147(3-4):447-51. DOI: 10.1016/S0176-1617(11)82181-4

Shetty K, Curtis O, Levin R. Specific interaction of mucoid strains of Pseudomonas spp. with Oregano (Origanum vulgare) clones and the relationship to prevention of hyperhydricity in tissue culture. J Plant Physiol. 1996;149(5):605-11. DOI: 10.1016/S0176-1617(96)80341-5

Strycharz S, Shetty K. Response of Oregano (Origanum vulgare L.) clonal lines to Pseudomonas sp. Z strain and polydye R-478 and implications for hyperhydricity prevention in tissue culture process. Biochemistry. 2002;38(3):343-50. DOI: 10.1016/S0032-9592(02)00073-0

Morone-Fortunato I, Avato P. Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum (Link) Ietswaart. Plant Cell Tissue Organ Cult. 2008;93(2):139-49. DOI: 10.1007/s11240-008-9353-5

Goleniowski M, Flamarique C, Bima P. Micropropagation of oregano (Origanum vulgare×applii) from meristem tips. In Vitro Cell Develop Biol Plant. 2003;39(2):125-8. DOI: 10.1079/IVP2002361

Yildirim M. Micropropagation of Origanum acutidens (HAND.-MAZZ.) IETSWAART using stem node explants. ScientificWorldJournal. 2013;2013:1-3. DOI: 10.1155/2013/276464

Kizil S, Khawar K. Efficient mass propagation of Origanum acutidens (HAND.-MAZZ.) IETSWAART under in vitro conditions. Bangladesh J Bot. 2017;46(2):667-73.

Murashige T, Skoog F. A Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant. 1962;15(3):473-97. DOI: 10.1111/j.1399-3054.1962.tb08052.x

Kunakh V. Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos; 2005. 730 p.

Kushnir G, Sarnacka V. Microclonal propagation of the plants. Kyiv: Naukova Dumka; 2005. 271 p.

Cherevchenko O, Lavrentyeva A, Ivannikov R. Biotechnology of tropical and subtropical plants in vitro. Kyiv: Naukova Dumka; 2008.

Weaver K, Morales V, Dunn S, Godde K, Weaver P. An introduction to statistical analysis in research: with application in the biological and life sciences. Wiley; 2017. 594 р. DOI: 10.1002/9781119454205

Lattanzio V, Cardinali A, Ruta C, Fortunato I, Lattanzio V, Linsalata V, et al. Relationship of secondary metabolism to growth in oregano (Origanum vulgare L.) shoot cultures under nutritional stress. Environ Exp Bot. 2009;65(1):54-62. DOI: 10.1016/j.envexpbot.2008.09.002

Derkach K, Abraimova O, Chernousova N, Smetanin V, Satarova T. Optimization of regeneration potential of maize Lancaster germplasm inbreds. Achiev Probl Genet Breed Biotechnol. 2012;4:465-70.


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