Research on the Possibility of Biotechnological Using Wastes of Fruit and Vegetable Products as a Substrate for Lentinula edodes
Keywords:Lentinus edodes, Grape pomace, Apple pomace, Bioconversion, Waste, Basidium fungi
Background. There are a number of interrelated problems: global hunger and malnutrition, depletion of natural resources, significant food losses and a significant increase in agro-industrial waste (agriculture and food processing). The use of agro-industrial waste as a substrate for the cultivation of basidium fungi is one of the ways to solve these problems. Popular edible mushroom shiitake (Lentinula edodes) is one of top three world leaders in terms of cultivation, so the development of technology for its cultivation using raw materials and crop waste is relevant for solving both environmental and nutrition problems.
Objective. The purpose of the paper is establishing the possibility of biotechnological use of waste from the processing of apples and grapes by bioconversion with fungi of the species L. edodes.
Methods. The principal growth potential at processing waste from apple and grape was investigated. Inoculation of sowing culture and determination of aphid speed were carried out according to standard methods. The method of Latin orthogonal rectangles was chosen to optimize the selection of a method for preparing a nutrient medium.
Results. L. edodes strain 22 showed the best result in growth rate on grape pomace, L. edodes strain 29 grew better on apple processing waste, L. edodes strain 24 showed an average result on all studied media, therefore, for further studies L. edodes strains 22 and 24 were selected. Using the method of Latin orthogonal rectangles, the following results were obtained: for L. edodes strain 22, grape non-extracted pomace are the most optimal medium for the preparation of the sowing material and for the main cultivation; for L. edodes strain 24, grape extracted pomace are the most optimal medium for the preparation of sowing material and for the main cultivation.Conclusions. Natural nutrient mediums provide a high growth rate of L. edodes cultures on various agar medium. Taking into account the growth rate at processing waste from apple and grape, two strains were selected for further research – 22 and 24. Using the proposed algorithms for the preparation of the seed material and the main substrate allows to reduce the substrate growth time by 20%, which not only reduces the entire cultivation preparation cycle by 10%, but also reduces the risk of contamination of the substrate by increasing the competitiveness of the Lentinula edodes.
Barshtein VY, еditor. Bioconversion of waste of agro-industrial complex. Novosibirsk: ANS "Si-BAK"; 2016. 88 p.
Koroban LP. Biological features of the mushroom Lentinus edodes (Berk.) Sing. with intensive cultivation on lignocellulosic waste in Moldova [dissertation]. Moscow; 2004. 133 p.
Minakov DV. Influence of ecological and biochemical parameters of bio-conversion of plant material on yield of biomass of fruit bodies of xylothrophic basidiomycetes [dissertation]. Moscow; 2018. 156 p.
Bogolytsyn KG. "Green" chemistry of lignin – new aspects of Arkhanskgelsk: AGTU; 2007. 21 р.
Tanashchuk TN. Development of biotechnology for edible fungus Lentinus edodes (Berk.) Sing. on wastes of wine-city processing [dissertation]. Yalta; 1997. 135 р.
Bisko NA, Babitskaya VG, Bukhalo AS, Krupoderova TA, Lomberg MV, Mikhailova OB, et al. Biological properties of medicinal macromycetes in culture. Kyiv: Altpress, 2011; 212 p.
Obolenskaya AV, El'nitskaya, ZP, Leonovich AA. Laboratory works on wood and cellulose chemistry. Moscow: Ecology; 1991. 320 p.
Kononov GN. Chemistry of wood and its main components. Moscow: MSFU; 1999. 247 p.
Bilai VI, еditor. Methods of experimental mycology. Kyiv: Naukova Dumka; 1982. 550 р.
Belova NV. Prospects for the use of biologically active compounds of higher basidiomycetes in Russia. Mycology and Phytopathology; 2004;38(2):1-5.
Rabinovich ML. Theoretical foundations of biotechnology of wood composites. Moscow: Science; 2001. Book 1, Wood and the mushrooms that destroy it; 262 p.
Kononyuk AE. Fundamentals of scientific research (general theory of experiment). Kyiv: Osvita Ukrainy; 2011. Book 1; 235 p.
Kononyuk AE. Fundamentals of scientific research (general theory of experiment). Kyiv: Osvita Ukrainy; 2011. Book 3; 228 p.
How to Cite
Copyright (c) 2019 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.IBB articles are published under Creative Commons licence:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.