DOI: https://doi.org/10.20535/ibb.2019.3.1.163106

Experimental and Theoretical Substantiation of the Express Method Development for Detection of Enteroviruses in Water by Surface Plasmon Resonance Method

Zinaida Klestova, Alla Yuschenko, Oksana Blotska, Volodymyr Maslov, Yurii Ushenin, Glib Dorozinsky, Serhii Kravchenko, Hanna Dorozinska

Abstract


Background. As a result of anthropogenic pollution and number of other factors, in drinking water can be present some viruses such as enterovirus and other pathogens that can be transmitted by water. Enteroviruses are capable of causing lesions of organs and systems of varying severity in humans, manifested by a number of clinical symptoms. For the detection of viruses appropriate research methods were developed, but they take a lot of time and require special reagents and equipment, that is significant drawback of sanitary-virological studies of drinking water quality.

Objective. The aim of the paper is to study the general possibility of using a surface plasmon resonance (SPR) phenomenon for detection of enteroviruses in water samples (swine Teschovirus A as an example) and to create on its basis a new express diagnostic method.

Methods. For implementation of a method for rapid detection of viral diseases pathogens in water by using SPR, the Plasmon-6 device was developed at the Institute of V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine. The research on the interaction of antigen and specific serum in various buffers was held at the State Scientific Control Institute of Biotechnology and Strains of Microorganisms.

Results. A general possibility of using SPR to detect enteroviruses in distilled and drinking water (swine Teschovirus A as an example) was theoretically substantiated and experimentally proved, and the express method for their detection was developed. It is shown that upon detection of pathogen in distilled water, the response of the SPR sensor is 1.57 times higher than in drinking water, which may be due to the influence of ionic composition in water on the interaction of the antigen with the antibody. The developed method allows real-time monitoring of changes in the test environment, significantly reduces the duration of enteroviruses detection and does not require the use of expensive nutrient media and reagents.

Сonclusions. The possibility of using the SPR method and the "Plasmon-6" device implementing this method for express detection of enteroviruses in distilled and drinking water was theoretically substantiated and experimentally proved.

Keywords


Enteroviruses; Surface plasmon resonance; Water; Detection of the pathogen; Rapid method

References


Gall AM, Mariñas BJ, Lu Y, Shisler JL. Waterborne viruses: a barrier to safe drinking water. PLoS Pathog. 2015;11(6). DOI: 10.1371/journal.ppat.1004867

WHO. Guidelines for drinking-water quality. 4th ed. Geneva: WHO Press; 2011.

Melnichenko OM, Mushtuk IU, Marchenko MI, Tashuta VS, Dremuh YuU, Klestova ZS. Enterovirus and their role in infectious pathology. Veterynarna Biotekhnolohia. 2014;24:105-14. Available from: http://nbuv.gov.ua/UJRN/vbtb_2014_24_22

Fong TT, Lipp EK. Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools. Microbiol Mol Biol Rev. 2005;69(2):357-71. DOI: 10.1128/mmbr.69.2.357-371.2005

Martynova GP. Enterovirus (nepolio) infection in children. Sibirskoe Meditsinskoe Obozrenie. 2014;3:100-5.

Sorokina MN, Skripnichenko NV. Viral encephalitis and meningitis in children: a guide for doctors. Moscow: Meditsina; 2004. 416 p.

Shevtsova NP, Golubeva MV. Neurological manifestations of enterovirus infection. Detskie Infektsii. 2004;3:49-52.

Doan SI, Bondarenko VI, Zadorozhnaya VI. Characteristic of enterovirus contamination of open water reservoirs. Voda i Vodoochisni Tehnologiyi. 2005;4:32-5.

Doan SI. Epidemiological analysis of the incidence of enterovirus infections, taking into account clinical manifestations, age and territorial distribution. Zaporozhskiy Meditsinskiy Zhurnal. 2006;2:62-7.

Rotbart HA. Meningitis and encephalitis. Human enterovirus infections. Washington: ASM Press;1995. p. 271-89.

Ischanova IL, Sagynbaeva BA, Umarova RA, et al. Modern clinical course of serous meningitis of enteroviral etiology. Vestnik Yuzhno-Kazahstanskoy Meditsinskoy Akademii. 2006;2:58-9.

DSanPiN 2.2.4-171-10. Hygienic requirements for drinking water intended for human consumption. Kyiv: Ministry of Justice of Ukraine; 2010. No. 452/17747.

Dorozinsky GV, Maslov VP, Ushenin YuV. Sensory devices based on surface plasmon resonance. Kyiv: Politekhnika; 2016. 264 p.

Bezruk ZD, Radov DG, Maslov VP, Dorozinsky GV, Dorozinska HV, Konchenko AV. Investigation of regularities of water purification by freezing method. Naukovyi Visnyk Kharkivskoho Politekhnichnoho Instytutu. 2016;50:137-41.

Dorozinska HV, Maslov VP, Dorozinsky GV. Modeling the SPR-sensor response to low concentrations of water nanosuspensions. J Multidisciplinary Eng Sci Stud. 2017;3(8):2007-11.

Romanenko VF, Soroka VI, Pruss OG. Recommendations for the diagnosis and control of enzootic encephalomyelitis (Teshen disease) of swine. Chernihiv; 1999. 16 p.

Dorozinsky GV, DoroshenkoTP, Maslov VP. Influence of technological factors on sensitivity of analytical devices based on surface plasmon resonance. J Sensor Technol. 2015;5(2):54-61. DOI: 10.4236/jst.2015.52006

Jiménez-Clavero MA, Fernández C, Ortiz JA, Pro J, Carbonell G, Tarazona JV, et al. Teschoviruses as indicators of fecal contamination of water. Appl Environ Microbiol. 2003;69(10):6311-5. DOI: 10.1128/aem.69.10.6311-6315.2003

Stenberg EB, Persson B, Roos H, Urbaniczky C. Quantitative determination of surface concentration of protein with surface plasmon resonance using radio labelled proteins. J Colloid Interface Sci. 1991;143(2):513-26. DOI: 10.1016/0021-9797(91)90284-f


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