The Stabilizing Effect of Magnetic Field for the Shape of Yeast Cells Saccharomyces cerevisiae on Silicon Surface
Keywords:Saccharomyces cerevisiae, Cells, Stabilization, Shape, Silicon, Magnetic field
Background. Development of methods of the targeted delivery of drugs in the nanocarriers with magnetic nanomaterials under the control of the magnetic field, industrial application of magnetically sensitive yeast, study of the viability and preservation of the biological activity of immobilized cells and the influence of various factors on the stabilization of these systems.
Objective. The aim of the work was to research the shape of yeast cells attached on the surface of silicon under influence of static magnetic field.
Methods. Cell suspension of 1-day’s culture of Saccharomyces cerevisiae in distilled water was inflicted on the surface of monocrystalline silicon plates of different types of conductivity. The cell pictures were observed under a microscope in reflected light after free drying in the air and storage samples in different modes.
Results. The results of experiments showed that in control samples irreversible destruction of the attached cells took place after 7 days of storage. If drying of cells occurred under the influence of static magnetic field during 10–97 days, the yeast cells looked intact. Even after stopping of magnetic field action, they saved practically an unchanging shape during more than two years in the ordinary terms of storage.
Conclusions. The rational mode of stabilization for shape of yeast cells on the surface of silicon was determined due to the influence of a magnetic field by induction of 0.17 T without introducing additional substances. The possible mechanism of enhancement of adhesion associated with the gettering of positively charged impurities on the surface of silicon and the increase of the electric potential under the action of a magnetic field was considered. The method of stabilization of cells, which are attached to the surface of the silicon in a magnetic field, may be useful in the manufacture of biochips with immobilized cells.
Gui Q, Lawson T, Shan S, Yan L, Liu Y. The application of whole cell-based biosensors for use in environmental analysis and inmedical diagnostics. Sensors. 2017;17(7):1623. DOI: 10.3390/s17071623
Anglin EJ, Cheng L, Freeman WR, Sailor MJ. Porous silicon in drug delivery devices and materials. Adv Drug Deliv Rev. 2008;60(11):1266-1277. DOI: 10.1016/j.addr.2008.03.017
Bonanno LM, de Louise LA. Whole blood optical biosensor. Biosens Bioelectron. 2007;23(3):444-448. DOI: 10.1016/j.bios.2007.05.008
Ghoshal S, Mitra D, Roy S, Majumder DD. Biosensors and biochips for nanomedical applications: a review. Sensors Transducers Journal, 2010;113(2):1-17.
Wu CC, Alvarez SD, Rang CU, Chao L, Sailor MJ. Label-free optical detection of bacteria on a 1-D photonic crystal of porous silicon. Proc SPIE 7167 Frontiers in Pathogen Detection from Nanosensors to Systems. 2009. DOI: 10.1117/12.809338
Digel IE, Zhubanova AA. The influence of water-soluble polymers on the adhesion of cells to solid surfaces. Biotehnologiya Teoriya i Praktika. 1998;1-2:122-3.
Digel IE. Effect of transition metal ions and water-soluble polymers on the attachment of yeast cells to solid surfaces [Internet]. earthpapers.net. 1998 [cited 2018 Oct]. Available from: http://earthpapers.net/vliyanie-ionov-perehodnyh-metallov-i-vodorastvorimyh-polimerov-na-prikreplenie-drozhzhevyh-kletok-k-tverdym-poverhnostyam
Fernandes P. Immobilization of cells with transition metal. In: Guisan JM, ed. Immobilization of enzymes and cells. 2nd ed. Humana Press; 2006. p. 367-72.
Safarik I, Pospiskova K, Maderova Z, Baldikova E, Horska K, Safarikova M. Microwave-synthesized magnetic chitosan microparticles for the immobilization of yeast cells. Yeast. 2015;32(1):239-243. DOI: 10.1002/yea.3017
Al-Qodah Z, Al-Shannag M, Al-Busoul M, Penchev I, Orfali W. Immobilized enzymes bioreactors utilizing a magneticfield: a review. Biochem Eng J. 2017;121(1):94-106. DOI: 10.1016/j.bej.2017.02.003
Safarik I, Safarikova M. Magnetically modified microbial cells: a new type of magnetic adsorbents. China Particuol. 2007;5(1-2):19-25. DOI: 10.1016/j.cpart.2006.12.003
Robatjazi SM, Shojaosadati SA, Khalilzadeh R, Farahani EV, Balochi N. Immobilization of magnetic modified Flavobacterium ATCC 27551 using magnetic field and evaluation of the enzyme stability of immobilized bacteria. Biores Technol. 2012;104:6-11. DOI: 10.1016/j.biortech.2011.11.035
Al-Hassan Z, Ivanova V, Dobreva E, Penchev I, Hristov J, Rachev R, et al. Non-porous magnetic supports for cell immobilization. J Ferment Bioeng. 1991;71(2):114-117. DOI: 10.1016/0922-338X(91)90234-8
Makara VA, Steblenko LP, Korotchenkov OA, Nadtochiy AB, Kalinichenko DV, Kurilyuk AN, et al. The features of magneto-stimulating change of surface electric potential in silicon crystals used for the needs of the solar energetics and microelectronics. Nanosyst Nanomater Nanotechnol. 2014;12(2):247-258.
Makara VA, Vasiliev MA, Steblenko LP, Koplak OV, Kurilyuk AN, Kobzar YL, et al. Variations in the impurity composition and microhardness of surface layers in silicon crystals caused by a magnetic field. Semiconductors. 2008;42:1044-1047. DOI: 10.1134/S106378260809008X
Gorobets SV, Gorobets OYu, Dvoynenko OK, Lebeda GL. The effect of magnetostatic fields of ferromagnetic substrate on the nickel dendrites electrodeposition. Naukovi Visti NTUU KPI. 2011;2:143-7.
Romanova ZM, Zubchenko VS, Tkachenko LV, Marynchenko LV. Effect of magnetic field on the activity of enzyme preparations [Internet]. Dspace.nuft.edu.ua. 2005 Apr [cited 2006]. Available from: http://dspace.nuft.edu.ua/jspui/bitstream/123456789/1289/1/EFFECT%20OF%20MAGNETIC%20FIELD%20ON%20THE%20ACTIVITY%20OF%20ENZYME%20PREPARATIONS.pdf
Zubchenko VS, Tkachenko LV, Protsan NV. Change alcohol yeast metabolism under magnetic field. Kharchova Promyslovist. 2009;8:22-24.
Berlot T, Rehar D, Fefer M, Berovic M. The influence of treatment of Saccharomyces cerevisiae inoculum with a magnetic field on subsequent grape must fermentation. Chem Biochem Eng Q. 2013;27(4):423-429.
Kotvetver VK, Korolev VG, Kutlakhmedov Yu.A., Evstikhina TA. A new type of radioprotectors based on the use of magnesium isotope magnesium-25. Naukovi Pratsi Tekhnohenna Bezpeka. 2012;185(173):54-8.
Yang X, Beckwith AW, Miller JH, Wood LT. Observation of magnetic field induced contraction of fission yeast cells using optical projection microscopy. Cent Europ J Phys. 2004;2(4):636-644. DOI: 10.2478/BF02475566
Litvinov GS, Polischuk VP, Boiko AL. Bacteriophage T4 structure and biological function changes under influence of constant magnetic field. Biopolym Cell. 1992;8(1):46-51. DOI: 10.7124/bc.00030E
Chen C, Wang P, Li L. Applications of bacterial magnetic nanoparticles in nanobiotechnology. J Nanosci Nanotechnol. 2016;16(3):2164-2171. DOI: 10.1166/jnn.2016.10954
Gorobets SV, Medviediev O, Gorobets OY, Ivanchenko A. Biogenic magnetic nanoparticles in human organs and tissues. Prog Biophys Mol Biol. 2018;135:49-57. DOI: 10.1016/j.pbiomolbio.2018.01.010
Deegan RD, Bakajin O, Dupont TF, Huber G, Nagel SR, Witten TA. Capillary flow as the cause of ring stains from dried liquid drops. Nature, 1997;389(6653):827-829. DOI: 10.1038/39827
Nizhelska OI, Makara VA, Steblenko LP, Naumenko SM, Kuryluik AM, Krit OM, et al., inventors. Method of cell immobilization. Ukraine patent 120536. 2017 Nov 10.
Yakhno TA, Yakhno VG. Structural evolution of drying drops of biological fluids. Tech Phys. 2009;54(8):1219-1227. DOI: 10.1134/S1063784209080210
Vancauwenberghe V, di Marco P, Brutin D. Wetting and evaporation of a sessile drop under an external electrical field: A review. Colloids Surf A Physicochem Eng Asp. 2013;432:50-56. DOI: 10.1016/j.colsurfa.2013.04.067
Ye H, Curcuru A. Biomechanics of cell membrane under low-frequency time-varying magnetic field: a shell model. Med Biol Eng Comp. 2016;54(12):1871-1881. DOI: 10.1007/s11517-016-1478-9
How to Cite
Copyright (c) 2018 The Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.IBB articles are published under Creative Commons licence:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.