Some Observations on the Role of Water States for Biological and Therapeutical Effects


  • Massimo Scalia CIRPS (Interuniversity Center of Research for Sustainable Development), Italy
  • Pasquale Avino International Committee for Research and Study of Environmental Factors; Istituto Nazionale Assicurazione Infortuni sul Lavoro, Italy
  • Massimo Sperini Interuniversity Center of Research for Sustainable Development, Italy
  • Valentina Viccaro EU.NA.M. Institute, Italy
  • Albina Pisani International Committee for Research and Study of Environmental Factors, Italy
  • Vincenzo I. Valenzi Interuniversity Center of Research for Sustainable Development; International Committee for Research and Study of Environmental Factors; UNIGLOBUS University, Italy



Cell water, Water dynamic changes, Biological and clinical implications, Skin electric parameters, Electro Acupuncture of Voll, APEC 300 and high resolution measures, Drugs at very low concentration, Potential measurements and frequency spectrum, Memory of water.


The peculiar properties of water are recalled, among them the “memory of water”, underlining the behavior of cell water, whose “ordered” structure is probably due to the endogenous electromagnetic fields generated by organelles inside the cells, such mitochondria and microtubules. Biological and clinical implications of water dynamic changes are reported, mainly based on SEP (Skin Electric Parameters) analysis and a hypothesis that has been advanced about an “action at distance” exerted by low concentration drugs. Nowadays, regarding measures of electro-cutaneous parameters our research can resort to a device of advanced electronics, APEC 300, that, beside an Electro Acupuncture operational function, but with quantitative precise values, can detect micro variations on parameters of cell aqueous solutions, and those of the water too, when the analyzed object is solicited by an external electromagnetic weak field, as well as by a drug at a very low concentration. A draft of two possible lines of research is outlined in conclusions, one depending only on ourselves and regarding “water tests” in the context of the “memory of water” experiments. By the side of EIS experiments about conductivity and impedance spectroscopy, in this article very high-resolution measures are suggested, by means of APEC 300, of an important parameter, the potential; a kind of measurements less complex and more reliable regarding the device/object interference, to which can besides easily be associated a frequency spectrum in the extremely low frequency region. In this line of research, it is the first time, for what we know, that some measurements of the potential, with its frequency spectrum, have been carried out for water without and with stimulation (the latter represented by the action of a magnetic field). This first set of measures by APEC 300 reveals the region 0–0.5 Hz as one of special interest.

Author Biography

Massimo Scalia, CIRPS (Interuniversity Center of Research for Sustainable Development)

Bioelectromagnetics Section of CIRPS, Coordinator


Piccardi G. Sopra un nuovo fenomeno di natura elettrica e sopra un nuovo effetto presentato dai metalli. Gazzetta Chimica Italiana. 1938;68:246-63.

Piccardi G, Corsi ML. Sulla precipitazione del carbonato di calcio da acqua dura attivata ("T" od "R") e normale. Gazzetta Chimica Italiana. 1938;68(5):287-91.

Piccardi G, Fabrini E, Corsi ML. Sulla tensione superficiale dell'acqua attivata "T". Gazzetta Chimica Italiana. 1938;68(8):471-73.

Piccardi G. Nuovi effetti chimici e biologici dell'acqua attivata "T" ed "R". La Chimica e l'Industria. 1939;7:455-7.

Piccardi G. Sulla disincrostazione fisica delle caldaie e su di una relazione fra fenomeni ambientali ed alcuni fenomeni chimico fisici. Tecnica Italiana. 1951 Sett./Ott;5.

Piccardi G, Capel Boute C. Sui rapporti fra fenomeni ambientali e fenomeni chimico-fisici: primi risultati delle esperienze condotte a Bruxelles. Geofisica e Meteorologia. 1953;1(4-5).

Piccardi G, Cini R. Azione di un campo elettromagnetico della frequenza di 10 KHz sul test chimico: il problema dell'influsso degli atmosferici. Geofisica e Meteorologia. 1956;4(1-2).

Piccardi G. Sulla struttura dell'acqua e sull'influsso di campi elettromagnetici di bassa frequenza. La Ricerca Scientifica. 1959;29(6).

Manzelli P, Masini G, Costa MG. I Segreti dell'Acqua. L'opera scientifica di Giorgio Piccardi. 2nd ed. Roma: Di Renzo; 2012.

Davenas E, Beauvais F, Amara J, Oberbaum M, Robinzon B, Miadonna A, et al. Human basophil degranulation triggered by very dilute antiserum against IgE. Nature. 1988;333(6176):816-8. DOI: 10.1038/333816a0

Benveniste J. Further biological effects induced by ultra high dilutions. inhibition by a magnetic field. In: Endler P C, Schulte J, editors. Ultra high dilution. Kluwe Academic Publishers; 1994. p. 35-8. DOI: 10.1007/978-94-015-8342-8_5

Benveniste J, Aïssa J, Jurgens P, Hsueh W. Digital biology: Specificity of the digitized molecular signal. FASEB Journal. 1998;12:A412.

Benveniste J, Jurgens P, Hsueh W, Aïssa J. Transatlantic transfer of digitized antigen signal by telephone link. J Allergy Clin Immunol. 1997 Feb;99:S175.

Benveniste J, Thomas Y, Schiff M, Belkadi L, Jurgens P, Kahhak L. Activation of human neutrophils by electronically transmitted phorbol-myristate acetate. FASEB Journal. 2000;13(1):33-9. DOI: 10.1054/mehy.1999.0891

Arani R, Bono I, Del Giudice E, Preparata G. Qed coherence and the thermodynamics of water. Int J Modern Phys B. 1995;09:1813. DOI: 10.1142/S0217979295000744

Del Giudice E, Preparata G, Fleischmann M. QED coherence and electrolyte solutions. J Electroanalyt Chem. 2000;482(2):110-6. DOI: 10.1016/S0022-0728(00)00019-X

Widom A, Srivastava Y, Valenzi V. The biophysical basis of water memory. Int J Quantum Chem [Internet]. 2009 May 19 [cited 2018 May 22]. Available from:

Valenzi VI, Widom A, Swain J, Sivasubramanian S, Srivastava YN. Biological Aharonov-Bohm effects and electromagnetic communication signals from bacterial DNA. In: Proc X Int Conf "Cosmos and Biosphere" [Internet]; 2013 Sept. 23-28; Koktebel, Crimea, Ukraine. Available from:

Fröhlich H. Long range coherence and energy storage in biological systems. Int J Quantum Chem. 1968;2:641-9. DOI: 10.1002/qua.560020505

Fröhlich H. Evidence for coherent excitation in biological system. Int J Quantum Chem. 1983;23:1589-95. DOI: 10.1002/qua.560230440

Bonifacio R, Preparata G. Coherent spontaneous emission. Phys. Rev A. 1970;2(336). DOI: 10.1103/PhysRevA.2.336

Del Giudice E, Preparata G, Vitiello G. Water as a free electric dipole laser. Phys. Rev Lett. 1988;61(9):1085-8. DOI: 10.1103/PhysRevLett.61.1085

Cifra M, Pokorny J, Havelka D, Cucera M A. Electric field generated by axial longitudinal vibration modes of microtubule. Biosystems. 2010;100(2):122-31. DOI: 10.1016/j.biosystems.2010.02.007

Cifra M. Electrodynamic eigenmodes in cellular morphology. Biosystems. 2012;109(3):356. DOI: 10.1016/j.biosystems.2012.06.003

Pokorný Jiři, Pokorný Jan, Kobilková J. Postulates on electromagnetic activity in biological systems and cancer. Integr Biol (Camb). 2013;5(12):1439-46. DOI: 10.1039/c3ib40166a

Ball P. Here lies one whose name was writ in water. Nature [Internet]. 2007 Aug 8. Available from: DOI: 10.1038/news070806-6. 2007

Leonhardt H. Fundamentals of electroacupuncture according to Voll. Uelzen: Medizinisch Literarische Verlagesellschaft MBH; 1980.

Casimiro L, Barsnley L, Brosseau L, Milne S, Welch V, Tugwell P, et al. Acupuncture and electroacupuncture for the treatment of rheumatoid arthritis. Cochrane Database Syst. Rev. 2005 Oct 19;4. DOI: 10.1002/14651858.CD003788.pub2

Quispe-Cabanilas JG, Damasceno A, von Glehn F, Brandão CO, Damasceno BP, Silveira WD, et al. Impact of electroacupuncture on quality of life for patients with relapsing-remitting multiple sclerosis under treatment with immunomodulators: A randomized study. BMC Complement Altern Med. 2012 Nov 5;12:209. DOI: 10.1186/1472-6882-12-209

Silvério-Lopes S. Analgesia por acupuntura. Curitiba: Institute Brasileiro de Therapias e Ensimo; 2013. DOI: 10.7436/2013.anac.0

Valenzi VI, Grieco U, Garcia A, Marinelli F, Lozito A, Messina B. Cutaneous electrical phenomena in the evaluation of the curative properties of sand and marine environment: Preliminary observation. C.I.R.M. Researches. 1999;3/1.

Valenzi VI, Monaco G, Spada S, Cimaglia P, Petraccia L, Messina B. Ruolo dei SEP (Skin electric Parameters) nello studio delle meteoropatie. Annali di Medicina Interna, 2003;18:164.

Pisani A, Valenzi VI, Lucchetta MC, Grassi M, Serio A, Avino P, et al. SEP variations on subjects with chronic constipation in idropinic treatment. In: Proc VII Int Conf "Cosmos and biosphere"; 2007 Oct. 1-6; Sudak, Ukraine.

Spada S, Lopalco M, Quartieri G, Cimaglia P, Lucchetta MC, Campanella J, et al. Valutazione bioelettrica delle meteoropatie con i SEP (Skin Electric Parameters) [Internet]. 2004. Available from: CIFA_News_45.pdf

Valenzi V. L'asse sardo-siberiano per vincere il tempohttp [Internet]. 2017 Apr 24. Available from:

Valenzi VI, Roseghini ML. From drug intolerance to a SEP (Skin Electric Parameters) driven therapy. Some preliminar observation. Rivista di Biologia/Biology Forum. 2000;93:306-12.

Lazarou J, Pomeranz BH, Corey PN. Incidence of adverse drug reactions in hospitalized patients: a meta-analysis of prospective studies. JAMA. 1998;279(15):1200-5.

Smalwood RH, Brown BH. Non-invasive assessment of gastric activity. In: Rolfe P, editor. Non-invasive physiological measurements. Vol. 2. New York: Academic Press; 1983. P. 179-217.

Sacchini V. Electropotentials in the clinical assessment of neoplasia. The Breast. 1996;5:282-86. DOI: 10.1016/S0960-9776(96)90027-2

Itsekson A, Shepshelovich D, Kanevsky A, Seidman DS. Measurement of electrical resistance of dermal-visceral zones as a diagnostic tool for gynecologic disorders. IMAJ. 2010;12:334-7.

Piccardi G, Capel-Boute C. The 22-year solar cycle and chemical tests. J Interdiscip Cycle Res. 1972;3(3-4):413-7. DOI: 10.1080/09291017209359357

Novikov VV, Zhadin MN. Combined action of weak constant and variable low-frequency magnetic fields on ionic currents in aqueous solutions of amino acid. Biophysics. 1994;39:41-5.

Montagnier L, Aïssa J, Ferris S, Montagnier JL, Lavallée C. Electromagnetic Signals Are Produced by Aqueous Nanostructures Derived from Bacterial DNA. Interdiscip Sci. 2009 Jun;1(2):81-90. DOI: 10.1007/s12539-009-0036-7

Montagnier L, Aïssa J, Del Giudice E, LavalléeC, TedeschiA, Vitiello G. DNA waves and water. JoP Conf Ser. 2011;306(1):1-11. DOI: 10.1088/1742-6596/306/1/012007

Giuliani L, D'Emilia E, Ledda M, Grimaldi S, Lisi A. New perspectives of Bioelectromagnetics in Biology and Medicine: DNA Spectra for diagnostic purposes. JoP Conf Ser. 2011;329:1-10. DOI: 10.1088/1742-6596/329/1/012011

Montagnier L, Del Giudice E, Aïssa J, Lavallée C, Vitiello G. Transduction of DNA information through water and electromagnetic waves. Electromagn Biol Med. 2015;34(2):106-12. DOI: 10.3109/15368378.2015.1036072

Kernbach S, Kernbach O. Reliable detection of weak emissions by EIS approach. Int J Unconvent Sci. 2016;E1:90-103.

Kernbach S. High-resolution electrochemical measurements for detecting effects of non-chemical treatment of water. In: Proc Conf Physics, Chemistry and Biology of Water; 2017 Oct 28; Sofia, Bulgaria.

Kernbach S, Kuksin I, Kernbach O. On accurate differential measurements with electrochemical impedance spectroscopy [Internet]. 2016 May 14. Available: arXiv:1607.07292 v1.[physics.ins-del]




How to Cite

Scalia M, Avino P, Sperini M, Viccaro V, Pisani A, Valenzi VI. Some Observations on the Role of Water States for Biological and Therapeutical Effects. Innov Biosyst Bioeng [Internet]. 2018Sep.26 [cited 2024Jun.13];2(3):149-62. Available from: