Realization of Macromycete Photoinduced Growth Activity: Influence of Cultivation Ways and the Concentration of Carbon and Nitrogen
Keywords:Macromycetes, Low-intensity light, Irradiation, Photoinduction, Growth activity
Background. Scientific principles of the biosynthetic activity photoregulation of edible and medicinal macromycetes.
Objective. The aim of the paper is to determine the influence of macromycete cultivation method, carbon and nitrogen concentration on the realization of their photoinduced growth activity.
Methods. Seed mycelium C. militaris, F. velutipes, H. erinaceus, L. edodes, and P. ostreatus irradiated with low-intensity laser light in different wavelength ranges at an energy dose of 230 mJ/сm2, was cultivated superficially on a liquid medium, and also by the deep cultivation method. As carbon source glucose was used, nitrogen – peptone at various concentrations. The biomass accumulation and glucose consumption efficiency were determined.
Results. It is proved that the realization degree of the photoinduction depends on the composition of the nutrient medium and the method of macromycete cultivation. It is established that in order to obtain the maximum stimulating effect after low-intensity laser radiation, a deep cultivation of the photoactivated seed mycelium should be carried out. A short-term low-intensity laser radiation results in a change in the trophism of macromycetes and is expressed in an increase in the biomass accumulation rate and in the efficiency of glucose consumption. Reducing the glucose concentration in the medium increases the photoinduced activity of the seed mycelium C. militaris, G. lucidum, L. edodes, H. erinaceus, and P. ostreatus. There was no significant effect of nitrogen concentration on photoinduced stimulation of macromycete growth.Conclusions. The results of the research are the basis for further studies of the influence of various factors on the photoinduction of the biosynthetic activity of edible and medicinal mushrooms to intensify the technological stages of their cultivation.
Zied DC, Pardo‐Giménez A, editors. Edible and medicinal mushrooms: Technology and applications. John Wiley & Sons Ltd; 2017. 562 р. DOI: 10.1002/9781119149446
Poyedinok NL. Using of artificial light in mushroom cultivation. Biotechnologia Acta. 2013; 6(6):58-70. DOI: 10.15407/biotech6.06.058
Friedl MA, Kubicek CP, Druzhinina IS. Carbon source dependence and photostimulation of conidiation in Hypocrea atroviridis. Appl Environ Microbiol. 2008; 74(1):245-50. DOI: 10.1128/AEM.02068-07
Friedl MA, Schmoll M, Kubicek CP, Druzhinina IS. Photostimulation of Hypocrea atroviridis growth occurs due to a cross-talk of carbon metabolism, blue light receptors and response to oxidative stress. Microbiology. 2008;154(4):1229-41. DOI: 10.1099/mic.0.2007/014175-0
Schuster A, Kubicek CP, Friedl MA, Druzhinina IS, Schmoll M. Impact of light on Hypocrea jecorina and the multiple cellular roles of Envoy in this process. BMC Genomics. 2007;8(1):449. DOI: 10.1186/1471-2164-8-449
Tisch D, Kubicek CP, Schmoll M. The phosducin-like protein PhLP1 impacts regulation of glycoside hydrolases and light response in Trichoderma reesei. BMC Genomics. 2011;12(1):613. DOI: 10.1186/1471-2164-12-613
Tisch D, Schmoll M. Targets of light signalling in Trichoderma reesei. BMC Genomics. 2013;14(1):657. DOI: 10.1186/1471-2164-14-657
Tisch D, Kubicek CP, Schmoll M. Cross roads between light response and nutrient signalling: ENV1 and PhLP1 act as mutual regulatory pair in Trichoderma reese. BMC Genomics. 2014;15:425-38. DOI: 10.1186/1471-2164-15-425
Schmoll M. Light, stress, sex and carbon – The photoreceptor ENVOY as a central checkpoint in the physiology of Trichoderma reesei. Fungal Biology. 2017;122(6):479-86. DOI: 10.1016/j.funbio.2017.10.007
Hill EP. Effect of light on growth and sporulation of Aspergillus ornatus. Microbiology. 1976;95(1):39-44. DOI: 10.1099/00221287-95-1-39
Zhu JC, Wang XJ. Effect of blue light on conidiation development and glucoamylase enhancement in Aspergillus niger. Acta Microbiologica Sinica. 2005;45:2275-78.
Ricci M, Krappmann D, Russo VEA. Nitrogen and carbon starvation regulate conidia and protoperithecia formation of Neurospora crassa grown on solid media. Fungal Genetics Report. 1991;38(1):87-8. DOI: 10.4148/1941-4765.1462
Sommer T, Degli-Innocenti F, Russo EA. Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa. Planta. 1987;170(2):205-8. DOI: 10.1007/BF00397889
Correa A, Lewis ZA, Greene AV. Multiple oscillators regulate circadian gene expression in Neurospora. PNAS. 2003;100(23):13597-602. DOI: 10.1073/pnas.2233734100
Bisko NA, Lomberg ML, Mytropolska NYu, Mykchaylova OB. The IBK mushroom culture collection. Kyiv: Alterpres; 2016. 120 p.
Babitskaya VG, Shcherba VV, Puchkova TA, Poyedinok NL. Effect of the conditions for a medicinal fungus Ganoderma lucidum (Curt.:Fr.) P.Karst (Rei Shi) on the polysaccharide production. Biotehnologiya. 2007;6:34-42.
Mykchaylova OB, Poyedinok NL, Buchalo AS. Biotechnological aspects of cultivation of species of the genus Morchella on liquid nutrient media. Immunopathology, Allergology, Infectology. 2009;2:166-7.
Puchkova TA, Kapich AN, Osadchaya OV. Influence of cultivation conditions on the production of biologically active substances by fungi of the genus Cordyceps and their antioxidant activity. Trudyi BGU. 2013;8(1):246-52.
Poyedinok NL, Mykchaylova OB, Buchalo AS, Negriyko AM. Light regulation of growth and biosynthetic activity of ling zhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt: Fr.) P. Karst. (Aphyllophoromycetideae) in pure culture. Int J Med Mushr. 2008;10(4):369-78. DOI: 10.1615/IntJMedMushr.v10.i4.100
Poyedinok NL, Mykchaylova OB, Tugay T. Effect of light wavelengths and coherence on growth, enzymes activity and melanin production of liquid cultured Inonotus obliquus (Ach.: Pers.). Pilát Appl Biochem Biotechnol. 2015; 176(2):333-43. DOI: 10.1007/s12010-015-1577-3
How to Cite
Copyright (c) 2018 Authors
This work is licensed under a Creative Commons Attribution 4.0 International License.
The ownership of copyright remains with the Authors.
Authors may use their own material in other publications provided that the Journal is acknowledged as the original place of publication and National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute” as the Publisher.
Authors are reminded that it is their responsibility to comply with copyright laws. It is essential to ensure that no part of the text or illustrations have appeared or are due to appear in other publications, without prior permission from the copyright holder.IBB articles are published under Creative Commons licence:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under CC BY 4.0 that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.