DOI: https://doi.org/10.20535/ibb.2018.2.2.132515

Formation and Properties Polymer Nanolayers to Enhance Cell Growth in vitro

Oksana Shtapenko, Ivan Hevkan, Yuriy Slyvchuk, Vasyl Syrvatka, Natalia Matvienko

Abstract


Background. Cultivation of cell cultures on synthetic coating makes it possible to obtain a complex spatially organized cellular system that enhances cell attachment and determines all further processes of differentiation, proliferation, and formation of extracellular matrix. It is necessary to examine properties of coatings, particularly, such as biocompatible polymers with cells, as they can be applied for various biological and medical applications.

Objective. We investigated the effects of glass surfaces modified with dextran, APTES, albumin and they compositions on the proliferation and metabolic activity of B16F10 cells.

Methods. Cellular line B16F10 were cultured in DMEM medium supplemented with 10 % fetal calf serum, 1 % penicillin-streptomycin in 5 % CO2 at 37 °C for 72 h. Cells were seeded at the glass plates, which modified nanolayers in various combinations: control group – glass, glass/APTES, glass/APTES/dextran, glass/APTES/albumin, glass/albumin, glass/APTES/dextran/albumin. The influence of the surface properties on the proliferation of B16F10culture and its viability was analyzed after every 24 h of incubation. The cultural medium was collected after 24, 48, and 72 h of cultivation for investigation lactate dehydrogenase activity.

Results. The high viability and proliferation growth of cells on APTES, albumin, and APTES/dextran/albumin coating were higher if compared with growth of cells on a glass surface. Improved the proliferation of the B16F10 cells was observed onto albumin (P < 0.001) and APTES/dextran/albumin (P < 0.001) nanolayers on 48 and 72 h, in contrast to to control and other experimental groups. Whereas, the difference between the number of cells grown on glass and APTES coating increases only on 72 h of cultivation.

Conclusions. Obtained results have shown that the glass surface modified albumin and APTES/dextran/albumin resulted in improving the viability and cell proliferation of B16F10cell line and can be used as a 3D system for cultivation of cells of different types.

Keywords


Cell culture; Proliferative activity; Surface; Nanolayers

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