Colloidal Characteristics of Water Systems of Rhamnolipid Biocomplex of Strain Pseudomonas Sp. Ps17 With Tween-80 and Their Prospects for Biotechnology

Elena Karpenko, Vladislav Voloshynets, Ilona Karpenko, Tetyana Pokynbroda, Ihor Semenyuk, Halyna Midyana


Background. Efficiency of mixed systems of biogenic and synthetic surfactants, based on their colloidal chemical properties, in environmentally safe technologies.

Objective. The aim of the paper is the study of the aqueous systems’ colloidal characteristics of the rhamnolipid biocomplex with Twееn-80 nonionic surfactant, as well as the study of the obtained mixtures’ action for the emulsion stabilization and for plant growth stimulation.

Methods. The surface tension of RBC, Tween-80, and their mixture solutions was measured by the Du-Nui method (with a platinum ring). The dependence of the surface tension on the surfactant concentration was determined for mixtures with RBC content, %: 0.0; 11.1; 25.0; 42.9; 66.7; 100. The emulsifying activity of the surfactant mixtures was determined on the emulsification index (E24). The surfactant influence on plants was assessed by their morphometric parameters after presowing seed treatment.

Results. It was found that the obtained mixture behavior, regardless of the surfactant concentration, deviates from the ideal mixture – the deviation is negative, which indicates the predominance of RBC in the surface layer. The shapes of the surface tension curves for different surfactant ratios indicate synergistic effects before and after micelle formation. The optimum ratio in the RBC-Tween-80 system for sunflower growth and sunflower oil emulsification is 2:1.

Conclusions. The peculiarities of colloid-chemical characteristics of RBC and Tween-80 systems are determined, the prospects of their use for increasing efficiency of emulsification and sunflower growth stimulation are determined.


Surface activity; Rhamnolipid biocomplex; Twееn-80; Surfactant systems; Еmulsification; Plant growth stimulation


Soboleva ОА, Kryvobokova МV. Mixed micelles and adsorption layers of a nonionic surfactant with a cationic (monomeric and dimeric). Vestnik Moskovskogo Universiteta. Ser. 2. Himiya. 2004;45(5):344-50.

Holland HM, Rubi DN. Mixed surfactant systems. In ACS Symp Ser. vol. 501. Washington: American Chemical Society; 1992. DOI: 10.1021/bk-1992-0501.ch001

Rosen MJ. Phenomena in mixed surfactant systems. In ACS Symp. Ser. vol. 311. Washington: American Chemical Society; 1986.

Hrabarovs'ka AP, Voloshynets' VA, Semenyuk IV, Karpenko OV. Colloidal properties of synthetic emulsifiers and rhamnolipid. Voprosy Himii i Himicheskoy Tehnologii. 2008;6:149-52.

Harlov A, Sakvarelydze M, Yampol'skaya H. Properties of mixed monomolecular layers of bovine serum albumin and nonionic surfactant Tween-80. Proc MCVI. 2007. p. 54-64.

Prieto C, Calvo L. Performance of the biocompatible surfactant Tween 80, for the formation of microemulsions suitable for new pharmaceutical processing. J Appl Chem. 2013;2013. DOI: 10.1155/2013/930356

Karpenko OV, Voloshynets' VA, Semenyuk IV, Karpenko AY. Colloid-chemical characteristics of the products of biosynthesis of Pseudomonas sp. PS-17. Voprosy Himii i Himicheskoy Tehnologii. 2012;2:30-4.

Kłosowska-Chomiczewska I, Mędrzycka K, Hallmann E, Karpenko E, Pokynbroda T, Macierzanka A. Rhamnolipid CMC prediction. J Colloid Interf Sci. 2017;488:10-9. DOI: 10.1016/j.jcis.2016.10.055

Sachdev DP, Cameotra SS. Biosurfactants in agriculture. Appl Microbiol Biotechnol. 2013;97:1005-16. DOI: 10.1007/s00253-012-4641-8

Brocklehurst PA, Dearman J. A comparison of different chemicals for osmotic treatment of vegetable seed. Ann Appl Biol. 1984;105:391-8. DOI: 10.1111/j.1744-7348.1984.tb03064

Humpherson-Jones FM. Effect of surfactants and fungicides on clubroot (Plasmodiophora brassicae) of brassicas. Ann Appl Biol. 1993;122:457-65. DOI: 10.1111/j.1744-7348.1993.tb04049.x

Karpenko IV, Midyana HH, Karpenko OY, Baranov VI. Influence of biogenic surfactants on the oilseed growth. Bulletin of Lviv Polytechnic National University, Chemistry, Technology of Substances and their Application. 2014;787:254-7.

Karpenko OV, Korets'ka NI, Shchehlova NS, Karpenko IV, Baranov VI. Growth stimulation of Gramineae plants by rhamnolipid surfactants. Biotechnologia Acta. 2013;6:94-9.

Karpenko I, Midyana H, Karpenko O. Biogenic rhamnolipid surfactants in complex plant growth regulators. Naukovi Visti NTUU KPI, 2016;3:36–41. DOI: 10.20535/1810-0546.2016.3.65576

Karpenko IV, Midyana HH, Karpenko OY, Tymchuk IC, Baranov VI. Prospects of rhamnolipid surfactants in technologies for sunflower growing. Bulletin of Lviv Polytechnic National University. Chemistry, Technology of Substances and their Application. 2016;841:164-8.

Batta YA. Invert emulsion: Method of preparation and application as proper formulation of entomopathogenic fungi. MethodsX. 2016;3:119-27. DOI: 10.1016/j.mex.2016.02.001

Standard Ukraine. Agricultural Crop Seeds. Methods for Determining Quality. AS 4138–2002. 2003.

Adler YuP, Markova EV, Hranovskyy YV. Experiment Planning in the Search for Optimal Conditions. Moscow: Nauka; 1976. 380 p.

Nyzhnyk VV, Voloshynets' VA, Nyzhnyk TY. Colloid Chemistry with Elements of Nanochemistry. Kyiv: Fitosotsiotsentr; 2012. 506 p.

GOST Style Citations



1. Isolation of Surfactants Synthesized by the Pseudomonas Bacteria and Study of Their Properties
Tetyana Pokynbroda, Ilona Karpenko, Halyna Midyana, Oleksandr Karpenko
Innovative Biosystems and Bioengineering  Vol: 3  Issue: 2  First page: 70  Year: 2019  
doi: 10.20535/ibb.2019.3.2.165838

Copyright (c) 2018 Igor Sikorsky Kyiv Polytechnic Institute

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.