Investigation of Safety and Efficacy of Antipediculosis Products on the Basis of Essential Oils


  • Dmytro Gavrylenko UA “PRO-PHARMA”, LLC, Ukraine
  • Tetyana Gots UA “PRO-PHARMA”, LLC, Ukraine
  • Iryna Kurganova State Enterprise «Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine», Ukraine
  • Natalia Chipak State Enterprise «Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine», Ukraine
  • Alexander Galkin Igor Sikorsky Kyiv Polytechnic Institute; UA “PRO-PHARMA”, LLC, Ukraine



Essential oils, Ovicidal properties, Pediculosis


Background. Despite the fact that the problem of pediculosis in children and adults exists for a long time, it does not lose its relevance. The relevance of the pediculosis is due to the fact that everyone can easily get infected with this disease, but infants are infected more often. Pediculosis is an indicator of social disadvantage, it is a socio-psychological problem for the patients and those around them, and can also create epidemic disadvantages in society since these lice can be of pathogen carriers of dangerous diseases. Therefore, the development of means for prevention and control of lousiness, in particular, the development of new pediculicids based on natural essential oils, is a further urgent task.

Objective. The aim of the paper is to study and prove the insecticidal (pediculicidal) and ovicidal activity of hair lotions made on the basis of lavender and ylang-ylang natural essential oils in laboratory conditions and to assess the safety of lotions when used in infected patients.

Methods. The method of biological quality control has been used to the study of hair lotions made on the basis of lavender and ylang-ylang natural essential oils to determine their biological activity against the lice of the Pediculus humanus humanus laboratory population on the laboratory base of rickettsia infections of the Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine. The study of the safety of lotions was carried out with the involvement of healthy volunteers and patients with pediculosis on theKiev city disinfection station base (department of chamber disinfection and sanitary passersby).

Results. The model formula for lotion made using 3.5 % lavender oil and 3.5 % ylang-ylang oil, causes 100 % death of the lice imago during exposure for 30 minutes. At less exposure, the lice imago death is reduced. Ovicidal properties are expressed during exposure for 30 min. The product is safe for humans, well tolerated and provides effective death of the lice, regardless of the patient infesting degree.

Conclusions. The results of the study of the model formulations of lotions indicate their high insecticidal and ovicidal activity in relation to the lice of the laboratory population when immersing test-insects in one of the studied lotions and adding the test-insects to the filter paper, moistened with one of the indicated lotions, with an exposure of 30 min. The product does not require additional safety measures and may be used in accordance with the directions for use.

Author Biographies

Dmytro Gavrylenko, UA “PRO-PHARMA”, LLC

Дмитро Гавриленко

Tetyana Gots, UA “PRO-PHARMA”, LLC

Тетяна Гоц

Iryna Kurganova, State Enterprise «Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine»

Ірина Курганова

Natalia Chipak, State Enterprise «Lviv Research Institute of Epidemiology and Hygiene of the Ministry of Health of Ukraine»

Наталія Чіпак

Alexander Galkin, Igor Sikorsky Kyiv Polytechnic Institute; UA “PRO-PHARMA”, LLC

Олександр Юрійович Галкін


Report on individual infections and parasitic diseases. 2010-2016. Form no. 1. Kyiv: Ministry of Health ofUkraine; 2016. 54 p.

Nekrasova LS, Svita VМ, Novokhatniy YО, Nesterenko LP, Rudenko ОY. The epidemic situation of especially dangerous infections inUkrainefor the period from 1998 to 2007. In: Actual problems on prevention of especially dangerous infections and biological safety.Simferopol; 2008. p. 3-7.

Kurganova IІ, Kitsara МS, Murzova LІ, Burtnyak ТV, Balakliets ТІ, Ponomaryova NV. Peculiarities of the spread of pediculosis among children inUkraine(1990–2004). Annals of Mechnicov Institute. 2007;1:13-8.

Chipak NІ, Toporovich ОІ, Kushnir ZG. The current state of the disease of pediculosis inUkraine. Proc. Scientific forum and workshop on the review and selection of scientific works with the support of the CESD within the framework of the concept of Unified Health inUkraine. 2017. p. 120.

Kurt Ö, Balcıoğlu IC, Limoncu ME, Girginkardeşler N, Arserim SK, Görgün S, et al. Treatment of head lice (Pediculus humanus capitis) infestation: Is regular combing alone with a special detection comb effective at all levels? Parasitol Res. 2015 Apr;114(4):1347-53. DOI: 10.1007/s00436-015-4311-8

Smith CH, Goldman RD. An incurable itch. Can Fam Physician. 2012 Aug; 58(8):839-41.

Burgess IF. Head lice. BMJ Clin Evid. 2011 May; 2011:1703.

Kurganova II, Klimchuk МD, Zarytskiy АМ. Methodical instructions regarding the unified methods of conducting biological control of anti-pediculosis remedies. Methodical instructions No: 5.09.19 – 604. Kyiv; 2000. 17 p.

Kolyadenko NV. Principles of bioethics as the moral and legal basis for the development of the health care system inUkraine. Journal of theAcademyofAdvocacyofUkraine. 2014;1:147-8.

Lis-Balchin M. Lavender: The genus Lavandula.London, CRC Press 2002; p. 208-9.

Cavanagh HM, Wilkinson JM. Biological activities of lavender essential oil. Phytother Res. 2002 Jun;16(4):301-8. DOI: 10.1002/ptr.1103

Perry N, Perry E. Aromatherapy in the management of psychiatric disorders clinical and neuropharmacological perspectives. CNS Drugs. 2006;20(4):257-80

Hudson R. The value of lavender for rest and activity in the elderly patient. Complement Ther Med. 1996 Jan;4(1):52-7. DOI: 10.1016/S0965-2299(96)80057-4

Field T, Cullen C, Largie S, Diego M, Schanberg S, Kuhn C. Lavender bath oil reduces stress and crying and enhances sleep in very young infants. Early Hum Dev. 2008 Jun;84(6):399-401. DOI: 10.1016/j.earlhumdev.2007.10.008

Goel N, Kim H, Lao RP. An olfactory stimulus modifies nighttime sleep in young men and women. Chronobiol Int. 2005;22(5):889-904. DOI: 10.1080/07420520500263276

Holmes C, Hopkins V, Hensford C, MacLaughlin V, Wilkinson D, Rosenvinge H. Lavender oil as a treatment for agitated behaviour in severe dementia: A placebo controlled study. Int Geriatr Psychiatry. 2002;17(4):305-8. DOI: 10.1002/gps.593

Evandri MG, Battinelli L, Daniele C, Mastrangelo S, Bolle P, Mazzanti G. The antimutagenic activity of Lavandula angustifolia (lavender) essential oil in the bacterial reverse mutation assay. Food Chem Toxicol. 2005;43(9):1381-7. DOI: 10.1016/j.fct.2005.03.013

Takarada K, Kimizuka R, Takahashi N, Honma K, Okuda K, Kato T. A comparison of the antibacterial efficacies of essential oils against oral pathogens. Oral Microbiol Immunol. 2004 Feb;19(1):61-4. DOI: 10.1046/j.0902-0055.2003.00111x

Nikolayevsky VV, Yeremenko AE, Ivanov IK. Biological activity of essential oils. Мoscow: Мedicina; 1987. 144 p.

Styx V, Weigerstorfer U. In the kingdom of odors. Essential oils and their effect. Мoscow: Naveus; 1997. 130 p.

Тisserand M. Aromatherapy for women.Moscow: Cron-press; 2000. 176 p.

Tan LT, Lee LH, Yin WF, Chan CK, Abdul Kadir H, Chan KG, et al. Traditional uses, phytochemistry, and bioactivities of Cananga odorata (Ylang-Ylang). Evid Based Complement Alternat Med. 2015;2015:896314. DOI: 10.1155/2015/896314

Jung DJ, Cha JY, Kim SE, Ko IG, Jee YS. Effects of Ylang-Ylang aroma on blood pressure and heart rate in healthy men. J Exerc Rehabil. 2013;9(2):250-5. DOI: 10.12965/jer.130007

Kusuma IW, Murdiyanto, Arung ET, Syafrizal, Kim Y. Antimicrobial and antioxidant properties of medicinal plants used by the Bentian tribe from Indonesia. Food Science and Human Wellness. 2014 Sept-Dec;3(3-4):191-6. DOI: 10.1016/j.fshw.2014.12.004

Andrade BFMT, Barbosa LN, Probst IS, Júnior AF. Antimicrobial activity of essential oils. J Essential Oil Res. 2014; 26(1):34-40. DOI: 10.1080/10412905.2013.860409

Rahman MM, Lopa SS, Sadik G, Harun-Or-Rashid, Islam R, Khondkar P, et al. Antibacterial and cytotoxic compounds from the bark of Cananga odorata. Fitoterapia. 2005;76(7-8):758-61. DOI: 10.1016/j.fitote.2005.08.011

Cardo MV, Vezzani D, Carbajo AE. Oviposition strategies of temporary pool mosquitoes in relation to weather, tidal regime and land use in a temperate wetland. Bulletin Entomolog Res. 2012 Dec;102(6):651-62. DOI: 10.1017/S0007485312000259

Soonwera M. Larvicidal and oviposition deterrent activities of essential oils against house fly (Musca domestica L.; Diptera: Muscidae). J Agricult Technol. 2015;11(3):657-67.

Choi EM, Hwang JK. Screening of Indonesian medicinal plants for inhibitor activity on nitric oxide production of RAW264.7 cells and antioxidant activity. Fitoterapia. 2005 Mar;76(2):194-203. DOI: 10.1016/j.fitote.2004.11.010

Gonzalez Audino P, Vassena C, Zerba E, Picollo M. Effectiveness of lotions based on essential oils from aromatic plants against permethrin resistant Pediculus humanus capitis. Arch. Dermatol. Res. 2007 Oct;299(8):389-92. DOI: 10.1007/s00403-007-0772-7

Jadhav V, Kore A, Kadam VJ. In-vitro pediculicidal activity of Hedychium spicatum essential oil. Fitoterapia. 2007 Dec;78(7-8):470-3. DOI: 10.1016/j.fitote.2007.02.016

Chipak NI, Kushnir ZG, Kitsara MS, Toporovych OI. The epidemiological situation of pediculosis in Ukraine in 2013 and the study of new insecticidal drugs. Proc. 12th Scientific-practical conference with international participation, dedicated to the Day of Science, the 75th anniversary of the "LNIIEG MOH Ukraine", 105th anniversary of G.S. Mosing "Modern problems of epidemiology, microbiology, hygiene and tuberculosis". Lviv; 2015. p. 30-3.



How to Cite

Gavrylenko D, Gots T, Kurganova I, Chipak N, Galkin A. Investigation of Safety and Efficacy of Antipediculosis Products on the Basis of Essential Oils. Innov Biosyst Bioeng [Internet]. 2018Apr.2 [cited 2024Jun.13];2(1):11-2. Available from: