Bionformation Detection of Magnetic Nanoparticles Producers Among Iron- and Manganese-Oxidizing Bacteria

Svitlana Gorobets, Oleksandr Kravchenko, Maryna Bulaievska, Olena Panchenko

Abstract


Background. In Ukraine, more than 17 % of underground water intakes are classified as unfunded in terms of iron content and 4.4 % in terms of manganese content. The increased content of these elements leads to a deterioration in the organoleptic properties of drinking water, the formation of precipitation and overgrowing of the water supply networks due to the development of iron-oxidizing bacteria. Therefore, it is important to study the magnetic properties of iron- and manganese-oxidizing bacteria for the possibility of their use in magnetic separation technologies in order to improve water treatment technologies with excess iron and manganese content.

Objective. The aim of the paper is to search for potential producers of BMN among iron- and manganese-oxidizing bacteria and classify them by an internal structure (crystalline or amorphous) and BMN allocation (extracellular or intracellular), using comparative genomics methods.

Methods. To evaluate the degree of similarity of BMN biomineralization proteins in MTB Magnetospirillum gryphiswaldense MSR-1 and in iron- and manganese-oxidizing bacteria, pair and multiple alignment methods using the free access program BLAST of the National Center for Biotechnological Information were used.

Results. The bioinformatic analysis showed that among the 30 investigated iron- and manganese-oxidizing bacteria which belong to 5 genera (Gallionella, Siderocapsa, Sphaerotilus, Hyphomicrobium, Leptothrix), 28 microorganisms are potential producers of BMN.

Conclusions. Conclusions are made about the prospects of further investigation of the influence of the magnetic field on the microorganisms of genera: Leptotrix, Sphaerotillus, Gallionella, Hyphomicrobium. An important direction for further experiments is the study of possible ways of conducting magnetic separation of iron- and manganese-oxidizing bacteria to prevent secondary contamination of drinking water and the formation of scales in pipelines. The use of the magnetic properties of the investigated microorganisms is useful for the development of ways to remove old scales within pipelines.

Keywords


Iron- and manganese-oxidizing bacteria; Biogenic magnetic nanoparticles; Biomineralization; Magnetospirillum gryphiswaldense MSR-1; Mam-proteins; Water purification

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DOI: https://doi.org/10.20535/ibb.2018.2.2.124256

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