Development of Conditions of Submerged Biosynthesis of Landomycin A




Streptomyces cyanogenus S136, Biosynthesis, Antibiotic, Landomycin A, Technological parameters


Background. Development of the process and biotechnology of production of antitumor antibiotic with microbial origin – landomycin A.

Objective. Establishment of the rational parameters for the submerged biosynthesis of landomycin A during the cultivation in the laboratory conditions.

Methods. Cultivation of the producer Streptomyces cyanogenus S136 was performed on shaking devices in the flasks. Antibiotic was extracted from culture fluid with ethyl acetate; the content of antibiotic was determined by measuring optical density of the extract.

Results. Conditions and parameters for antibiotic submerged biosynthesis of the landomycin A by S. cyanogenus S136 were investigated. Alternative nutrient mediums for the biosynthesis of the landomycin A were presented. Mediums that are based on soybean’s flour extract and corn’s flour allow obtaining up to 80 mg/ml of antibiotic. The influence of mixing intensity and temperature on the rate of the product accumulation and its dynamics was established. Rational technological parameters of the producer’s submerged biosynthesis such as temperature 25 ± 1 °С mixing intensity 230–250 U/min, duration of the submerged fermentation 50–60 hours were established.

Conclusions. Variants of the culture media, that were selected (based on the corn’s flour and the extract of the soybean’s flour) and the parameters of the landomycin A biosynthesis, that were set are the basis for optimization and design of the technology for obtaining the antibiotic in industrial conditions.

Author Biographies

Tetiana Todosiichuk, National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»

Doctor of technical sci., assistant professor, head of the department

Victor Fedorenko, Ivan Franko National University of Lviv

Doctor of biological sci., professor, head of the department

Maryna Molochko, National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute»


Oleksandr Gromyko, Ivan Franko National University of Lviv

Ph.D, senior researcher


Novikov V, Sydorov Y, Shved O. Trends in commercial biotechnology. Visnyk NAN Ukrayiny. 2008;2:25-39.

Kostyuk RV. The development of innovative activities of the biotechnological companies in the modern conditions. Aktual'ni Problemy Ekonomiky. 2009;98(8):79-84.

Alyaev YG, Rapoport LM, Carichenko DG. The use of mitomycin in the treatment of scar complications after radical prostatectomy. Andrologiya i Genitalnaya Hirurgiya. 2013;2:19-25.

Viktorov AP, Matveeva EV. Modern antineoplastic drugs: problems. Zdorov’ya Ukrayiny. 2010 Apr;26-8.

Ostash B, Korynevska A, Stoika R, Fedorenko V. Chemistry and biology of landomycins, an expanding family of polyketide natural products. Mini Rev Med Chem. 2009 Aug;9(9):1040-51. DOI 10.2174/138955709788922593

Oskay M. Antifungal and antibacterial compounds from Streptomyces strains. African J Biotechnol. 2009 July 6;8(13):3007-17.

Yücel S, Yamaç M. Selection of Streptomyces isolates from Turkish karstic caves against antibiotic resistant microorganisms. Pak J Pharm Sci. 2010 Jan;23(1):1-6.

Sanchez S, Demain AL. Metabolic regulation of fermentation process. Enzyme Microb Technol. 2002 Dec 2;31(7):895–906. DOI 10.1016/S0141-0229(02)00172-2

Rafieenia R. Effect of nutrients and culture conditions on antibiotic synthesis. Asian J. Pharm. Hea. Sci. 2013 July-Sep;3:810-5.

Sarkar S. Enhanced antimicrobials production by Streptomyces sundarbansensis sp. nov. in a novel extended surface biofilm reactor. Int J Adv Biotechnol Res. 2015;6(1)12-20.

Dutta S, Basak B, Bhunia B, Chakraborty S, Dey A. Kinetics of rapamycin production by Streptomyces hygroscopicus MTCC 4003. 3 Biotech. 2014 Oct;4(5):523-531. DOI 10.1007/s13205-013-0189-2

Liaoa Y, Wei ZH, Bai L, Deng Z, Zhong JJ. Effect of fermentation temperature on validamycin A production by Streptomyces hygroscopicus 5008. J Biotechnol. 2009 Jul 15;142(3-4):271-4. DOI 10.1016/j.jbiotec.2009.04.015

Kumari M, Myagmarjav BE, Prasad B, Choudhary M. Identification and characterization of antibiotic producing actinomycetes isolates. Am J Microbiol, 2013;4(1):24-31. DOI 10.3844/ajmsp.2013.24.31

Bundale S, Begde D, Nashikkar N, Kadam T, Upadhyay A. Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. isolated from soil. Adv Microbiol. 2015;5(6):441-51. DOI 10.4236/aim.2015.56045

Luzhets'kyy A, Fedorenko V, Ostash I, Ostash B. A method of production landomycin A. Ukrainian; 84651, 2013.



How to Cite

Todosiichuk T, Fedorenko V, Molochko M, Gromyko O. Development of Conditions of Submerged Biosynthesis of Landomycin A. Innov Biosyst Bioeng [Internet]. 2017Dec.19 [cited 2024Apr.15];1(1):36-42. Available from: