Sensor System for Sulfamethoxazole Detection Based on Molecularly Imprinted Polymer Membranes


  • Tetyana A. Sergeyeva Institute of Molecular Biology and Genetics, Ukraine
  • Elena V. Piletska University of Leicester, United Kingdom
  • Larysa A. Gorbach Institute of Macromolecular Chemistry, Ukraine
  • Alena V. Ivanova Igor Sikorsky KPI, Ukraine
  • Oleksandr O. Brovko Institute of Macromolecular Chemistry, Ukraine
  • Ganna V. El’ska Institute of Molecular Biology and Genetics, Ukraine



Sensors, Sensor systems, Molecularly imprinted polymers, Membranes, Pharmaceuticals, Sulfanilamides


Background. Development of sensor systems based on synthetic mimics of biological molecules will provide new effective express-methods for detection of small organic molecules, including pharmaceuticals, for modern analytical biotechnology.

Objective. An analytical system for highly selective and sensitive detection of sulfamethoxazole based on molecularly imprinted polymer (MIP) membranes is proposed, synthesized using the method of in situ polymerization in a combination with the method of computational modeling.

Methods. Sulfamethoxazole molecules, that were selectively adsorbed by the synthetic binding sites in MIP membranes structure, were visualized due to their ability to form brown-colored complexes after reaction with potassium ferricyanide and sodium nitroprusside in alkaline media.

Results. The limit for sulfamethoxazole detection comprised 2 mM, while the linear dynamic range – 2–15 mM, which allows one to detect sulfamethoxazole in pharmaceutical preparations. Stability of the developed MIP-based sensor systems was estimated as at least 6 months, which significantly increases stability of analogous devices based on natural receptors.

Conclusions. Applicability of the developed sensor systems for the analysis of sulfamethoxazole in both model solutions and real samples (commercial pharmaceutical preparations) was proven. The developed systems are characterized with high selectivity, sensitivity, small size and low cost.

Author Biographies

Tetyana A. Sergeyeva, Institute of Molecular Biology and Genetics

Dr.sci., leading research fellow

Elena V. Piletska, University of Leicester

Ph.D, senior research fellow

Larysa A. Gorbach, Institute of Macromolecular Chemistry

Ph.D, senior research fellow

Alena V. Ivanova, Igor Sikorsky KPI


Oleksandr O. Brovko, Institute of Macromolecular Chemistry

Dr.sci., head of department

Ganna V. El’ska, Institute of Molecular Biology and Genetics

Dr.sci., professor, member of NASU, director


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How to Cite

Sergeyeva TA, Piletska EV, Gorbach LA, Ivanova AV, Brovko OO, El’ska GV. Sensor System for Sulfamethoxazole Detection Based on Molecularly Imprinted Polymer Membranes. Innov Biosyst Bioeng [Internet]. 2017Dec.19 [cited 2023Jun.9];1(1):11-7. Available from: