Sensor System for Sulfamethoxazole Detection Based on Molecularly Imprinted Polymer Membranes

Tetyana A. Sergeyeva, Elena V. Piletska, Larysa A. Gorbach, Alena V. Ivanova, Oleksandr O. Brovko, Ganna V. El’ska


Background. Development of sensor systems based on synthetic mimics of biological molecules will provide new effective express-methods for detection of small organic molecules, including pharmaceuticals, for modern analytical biotechnology.

Objective. An analytical system for highly selective and sensitive detection of sulfamethoxazole based on molecularly imprinted polymer (MIP) membranes is proposed, synthesized using the method of in situ polymerization in a combination with the method of computational modeling.

Methods. Sulfamethoxazole molecules, that were selectively adsorbed by the synthetic binding sites in MIP membranes structure, were visualized due to their ability to form brown-colored complexes after reaction with potassium ferricyanide and sodium nitroprusside in alkaline media.

Results. The limit for sulfamethoxazole detection comprised 2 mM, while the linear dynamic range – 2–15 mM, which allows one to detect sulfamethoxazole in pharmaceutical preparations. Stability of the developed MIP-based sensor systems was estimated as at least 6 months, which significantly increases stability of analogous devices based on natural receptors.

Conclusions. Applicability of the developed sensor systems for the analysis of sulfamethoxazole in both model solutions and real samples (commercial pharmaceutical preparations) was proven. The developed systems are characterized with high selectivity, sensitivity, small size and low cost.


Sensors; Sensor systems; Molecularly imprinted polymers; Membranes; Pharmaceuticals; Sulfanilamides


Trojanowicz M. Enantioselective electrochemical sensors and biosensors: A mini-review. Electrochem Commun. 2014 Jun;38:47-52. DOI 10.1016/j.elecom.2013.10.034

Vidal JC, Bonel L, Ezquerra A, Hernández S, Bertolín JR, Cubel C, et al. Electrochemical affinity biosensors for detec-tion of mycotoxins: A review. Biosens Bioelectron. 2013 Nov 15;49:146-58. DOI 10.1016/j.bios.2013.05.008.

Díaz-Díaz G, Antuña-Jiménez D, Blanco-López MC, Lobo-Castañón MJ, Miranda-Ordieres AJ, Tuñón-Blanco P. New materials for analytical biomimetic assays based on affinity and catalytic receptors prepared by molecular imprinting. Trends Analyt Chem. 2012 March;33:68-80. DOI 10.1016/j.trac.2011.09.011

Ulbricht M. Membrane separations using molecularly imprinted polymers. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 5;804(1):113-25.

Sergeyeva TA, Piletsky SA, Panasyuk TL, El’skaya AV, Brovko OO, Slinchenko EA, et al. Conductimetric sensor for atrazine detection based on molecularly imprinted polymer membranes. Analyst. 1999;124:331-4. DOI 10.1039/A808484J

Sergeyeva TA, Gorbach LA , Slinchenko OA, Goncharova LA, Piletska OV, Brovko ОО, et al. Towards development of colorimetric test-systems for phenols detection based on computationally-designed molecularly imprinted polymer membranes. Mater Sci Eng C. 2010 Apr;30(3):431-6.

Shewiyo DH, Dejaegher B, Vander Heyden Y. Validation of thin layer chromatographic methods. In: Instrumental Thin-Layer Chromatography. Elsevier; 2015. p. 351-73.

Rudy BC, Senkowski BZ. Sulfamethoxazole. Analytical Profiles of Drug Substances, 1973;2:467-86.

GOST Style Citations

Copyright (c) 2017 Igor Sikorsky Kyiv Polytechnic Institute

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.