@article{Porva_Rybalko_Dyadyun_Lutsenko_Galkin_Poholenko_Gorbatyuk_2017, title={Study of Antiviral Activity of Recombinant Human Interleukin-7 with Various Experimental Models of Hepatitis C Viral Infection}, volume={1}, url={http://ibb.kpi.ua/article/view/112852}, DOI={10.20535/ibb.2017.1.1.112852}, abstractNote={<p class="a"><strong>Background.</strong> Interleukin-7 (IL-7) is one of the most important regulatory cytokine of immune system. Given the ability of IL-7 to the modulation of T- and B-cell responses and T-cell homeostasis we may assume that IL-7 not only has the ability to influence the formation of specific immunity and immunodeficiency state, but also inhibit the reproduction of viruses, including hepatitis C virus (HCV).</p><p class="a"><strong>Objective.</strong> Study of antiviral activity of recombinant human IL-7 (rIL-7) with experimental models of viral hepatitis C infection <em>in vitro</em> on sensitive virus epithelial and lymphoid cells.</p><p class="a"><strong>Methods.</strong> We have used the following inoculated cell cultures: <em>Jurkat</em>, rat neurinoma of gasserian ganglion and bovine kidney. As used surrogate HCV we used virus of bovine diarrhea. To study the antiviral activity different concentrations of rIL-7 were injected into the cell culture, producing HCV, and determined virus load. Also we have performed cytological analysis of cells and determined its proliferative activity under influence of rIL-7.</p><p class="a"><strong>Results.</strong> It has been shown that rIL-7 inhibits surrogate HCV reproduction in <em>in vitro</em> conditions (SS<sub>50</sub> – 3 mg/ml, ED<sub>50</sub> – 4.7 ng/ml, IS – 640). Highest proliferation of intact T-cells is determined at rIL-7 doses 0.3 mg/m and 0.025 mg/ml. rIL-7 affected HCV infected cells differently: during the first 3 days the number of cells decreased or did not change, and after 2–3 weeks the number of cells increased almost 2 times. When we injected rIL-7 with dose of 6 mg/ml within 3 days we obtained 89% viral inhibition at the 3<sup>rd</sup> day and 100 % at the 4<sup>th</sup> day; using the dose of rIL-7 0.3 mg/ml the inhibition on the 4<sup>th</sup> day was 100 %; using dose of rIL-7 1.5 mg/ml the inhibition settled at 55 % for 4 days.</p><p class="a"><strong>Conclusions.</strong> As a result of the studies directed towards determining the effect of rIL-7 on surrogate HCV reproduction, HCV cDNA producing transfected human T-cells <em>Jurkat</em>, it was showed that rIL-7 effectively inhibits virus reproduction.</p>}, number={1}, journal={Innovative Biosystems and Bioengineering}, author={Porva, Yulia I. and Rybalko, Svitlana L. and Dyadyun, Svitlana T. and Lutsenko, Tetiana M. and Galkin, Alexander Yu. and Poholenko, Yanina A. and Gorbatyuk, Oksana B.}, year={2017}, month={Dec.}, pages={27–35} }